Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing mixed bacteria immobilized particle

A technology of mixing bacteria and particles, applied in the direction of bacteria fixed on or in inorganic carriers, can solve problems such as river water and sediment pollution, and achieve the effects of low cost, simplified process and fast adsorption speed.

Inactive Publication Date: 2010-09-29
SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The nitrobenzene-containing wastewater from the double benzene plant flows into the Songhua River, causing river water and sediment pollution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing mixed bacteria immobilized particle
  • Method for preparing mixed bacteria immobilized particle

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The method for immobilizing microorganisms on zeolite comprises the following steps: firstly, seed liquid is prepared, and at the same time, inorganic carrier zeolite is treated; Specific steps are as follows:

[0025] (1) Preparation of seed solution

[0026] Under facultative anaerobic conditions, add 160ml of enrichment medium to a 250ml anaerobic bottle, sterilize, add 18ml of seed solution after cooling, and put it in an incubator at 28°C to keep still at constant temperature Cultivate for one week to make the microorganisms in the logarithmic growth phase, and set aside.

[0027] The anaerobic bottle is a medical infusion bottle; the culture medium is calculated by weight percentage: glucose 0.1%, peptone 0.05%, yeast extract 0.05%, NaCl 0.5%, K 2 HPO 4 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O0.05%, pH7.2. The sterilization conditions are: 0.1Mpa damp heat sterilization for 30 minutes. Described seed liquid is the facultative anaerobic mixed microorganism tha...

Embodiment 2

[0037] The difference from Example 1 is:

[0038] (1) Preparation of seed solution

[0039] Under facultative anaerobic conditions, add 180ml of enriched medium into a 250ml anaerobic bottle, sterilize, add 16ml of seed solution after cooling, and put it in an incubator at 28°C for a week of constant temperature and static culture, so that the microorganisms are in the right condition. Number of growing seasons, spare.

[0040] The medium is calculated by weight percentage: glucose 0.2%, peptone 0.06%, yeast extract 0.06%, NaCl 0.6%, K 2 HPO 4 0.5%, KH 2 PO 4 0.3%, MgSO 4 ·7H 2 O0.05%, pH7.2. Sterilization, the sterilization conditions are: 0.1Mpa damp heat sterilization for 30 minutes. The seed solution is a facultative anaerobic mixed microorganism containing 2ml of nitrobenzene solution with a concentration of 3mg / ml, and the concentration of nitrobenzene is 30mg / L.

[0041] (2) Pretreatment of the carrier

[0042] Zeolite (purchased from the Faku zeolite mine in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method for mixed bacteria immobilization particles, in particular to a method which adopts zeolite as a carrier to immobilize the mixed bacteria. The preparation steps are that: prepared seed liquid is vaccinated to the carrier zeolite that is treated, under the condition of being facultative anaerobic, the seed liquid is cultured for 4 to 6 days in constanttemperature to do adsorption, culture and multiplication for 2 to 3 times, and then the zeolite particles immobilized with the mixed bacteria are obtained. The invention adopts the inorganic carrier zeolite to adsorb and immobilize the mixed bacteria with fast adsorption speed to a plurality of bacteria, good multiplication effect and simple operation, the natural environment does not need to be changed and large scale buildings do not need to be built and no secondary pollution is generated. The prepared immobilized particles are put into bottom mud of polluted water of nitrobenzene class toeffectively remove nitrobenzene and aniline from the bottom mud; when the original concentrations of the nitrobenzene and the aniline in the bottom mud are respectively 4.41 mg / kg and 37.84mg / kg, thedegradation rate of the nitrobenzene is up to 100 percent and the degradation rate of the aniline is 52.51 percent. The zeolite resource in the invention is rich with low price.

Description

technical field [0001] The invention relates to a preparation method of mixed bacteria immobilized granules, specifically using zeolite as a carrier to immobilize mixed bacteria and the immobilized granules to remove nitrobenzene pollutants in bottom mud. Background technique [0002] The development of microbial immobilization technology began in the 1970s, and many achievements have been made in the treatment of medicine, food, chemical industry and point source sewage, but the research on the remediation of polluted surface water, especially the remediation of sediment has not been reported. [0003] Microbial immobilization technology refers to the basic technology that uses chemical or physical methods to locate free cells (microorganisms) in a limited space area, maintain activity, and can be used repeatedly. Immobilization technology can fix intact cells, which can maintain the activity and stability of biological enzymes to the greatest extent, increase the density a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/20
Inventor 李培军鞠京丽张海荣张春桂许华夏
Owner SHENYANG INST OF APPL ECOLOGY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com