Biological treatment method for wood fiber hydrolysate

A lignocellulosic and biological treatment technology, applied in the field of biomass refining, can solve the problems of poor selectivity, high loss rate of oligosaccharides, low lignin removal efficiency, etc., to reduce removal rate, reduce loss, improve membrane flux and The effect of production efficiency

Inactive Publication Date: 2017-02-22
深圳中农秸美科技股份有限公司
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AI Technical Summary

Problems solved by technology

[0014] Aiming at the problem of low lignin removal efficiency and poor selectivity in the process of extracting oligosaccharides from the hydrolyzate of lignocellulosic raw materials by using cationic polymers in the process of extracting oligosaccharides from the hydrolyzate of the existing lignocellulosic raw materials, the present invention provides A kind of biological treatment method of lignocellulosic hydrolyzate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A kind of lignocellulosic hydrolyzate biological treatment method, the steps are as follows:

[0040] (1) The hydrolyzed liquid after self-hydrolysis of poplar wood, the content of oligosaccharides is 7.2g / L, the content of lignin is 4.5g / L, and the content of colloidal lignin is 1.4g / L. The hydrolyzate is treated with acid pectinase, and the treatment conditions are: the enzyme dosage is 150g / ton hydrolyzate, the temperature is 40°C, the time is 8h, and the pH is 3.5.

[0041] (2) The hydrolyzate after pectinase treatment is treated with cationic polymer polyallyldimethylammonium chloride, the amount of cationic polymer is 100g / ton of hydrolyzate, the treatment temperature is room temperature, pH3.5, and the time is 5 minutes. After the cationic polymer was treated, it was left to stand for 8 hours, and the precipitate was removed by decantation to obtain the purified hydrolyzate of the upper layer.

[0042] In this example, after pectinase treatment and cationic poly...

Embodiment 2

[0045] A kind of lignocellulosic hydrolyzate biological treatment method, the steps are as follows:

[0046] (1) The hydrolyzed solution after self-hydrolysis of wheat straw has an oligosaccharide content of 4.6 g / L, a lignin content of 3.1 g / L, and a colloidal lignin content of 0.8 g / L. The hydrolyzate is first treated with neutral pectinase, and the treatment conditions are: enzyme dosage 180g / ton of hydrolyzate, temperature 45°C, time 12h, pH 7.0.

[0047] (2) After pectinase treatment, cationic polymer polyethylenimine was used to treat the hydrolyzate at an amount of 85 g / ton, at room temperature, pH 7.0, and for 10 minutes. After the cationic polymer was treated, it was left to stand for 4 hours, and the precipitate was removed by decantation to obtain the purified hydrolyzate of the upper layer. After pectinase-cationic polymer treatment, the removal rate of lignin was 45%, among which the removal rate of colloidal lignin was 90%, and the loss rate of oligosaccharide w...

Embodiment 3

[0050] A kind of lignocellulosic hydrolyzate biological treatment method, the steps are as follows:

[0051] (1) The hydrolyzed solution after self-hydrolysis of spruce, the content of oligosaccharides is 6.8g / L, the content of lignin is 3.9g / L, and the content of colloidal lignin is 1.6g / L. The hydrolyzate is first treated with acid pectinase, and the treatment conditions are: the enzyme dosage is 200g / ton hydrolyzate, the temperature is 50°C, the time is 16h, and the pH is 4.0.

[0052] (2) After pectinase treatment, polyaluminum chloride is used for treatment, the dosage is 200 g / ton of hydrolyzate, the treatment temperature is room temperature, pH 4.0, and the treatment time is 15 minutes. After cationic polymer treatment, centrifuge for 15 minutes to remove the precipitate and obtain the purified hydrolyzate of the upper layer. After pectinase-cation polymer treatment, the removal rate of lignin was 47%, among which the removal rate of colloidal lignin was 91%, and the l...

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Abstract

The invention relates to a biological treatment method for wood fiber hydrolysate, and belongs to the field of biomass refining. The method comprises the steps that pectinase is added into hydrolysate obtained after a wood fiber raw material is self-hydrolyzed for enzyme treatment, wherein the using amount of the pectinase is 10-1,000 g per ton of the hydrolysate, the enzyme treatment temperature ranges from 30 DEG C to 70 DEG C, the enzyme treatment time ranges from 1 h to 48 h, and the pH ranges from 3.0 to 8.0; after the hydrolysate is treated with the pectinase, treatment is conducted by adopting a cationic polymer. According to the method, the removal efficiency of lignin, especially colloid lignin in the hydrolysate can be improved, the using amount of the cationic polymer can be decreased, and meanwhile loss of oligosaccharide in the cationic polymer treatment process can be significantly reduced.

Description

technical field [0001] The invention relates to a method for pretreatment of hydrolyzate, in particular to a method for biological treatment of lignocellulosic hydrolyzate, which belongs to the field of biomass refining. Background technique [0002] Lignocellulosic raw materials including wood and non-wood such as bagasse, reed and various grasses contain a large amount of hemicellulose glycans, which form oligosaccharides after proper degradation. These oligosaccharides have very important application value in food, medicine and chemical industry, therefore, how to separate hemicellulose from plant raw materials to obtain oligosaccharides is of great significance. At present, after self-hydrolysis of plant fiber raw materials, most of the hemicellulose can be hydrolyzed to form oligosaccharides, which are separated from the plant raw materials and entered into the hydrolyzate. No chemicals are added during the self-hydrolysis hydrolysis process, mainly relying on the fiber...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H3/06C07H1/08
CPCC07H1/08C07H3/06
Inventor 李宗全秦梦华傅英娟
Owner 深圳中农秸美科技股份有限公司
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