Feeding optimizing method for heterotrophically culturing chlorella

Abstract

An optimization method of supplementary food of heterotrophic culture of chlorella in the technical field of biological engineering comprises the steps that 1, heterotrophic culture of chlorella is conducted in stages in a fermentation cylinder, and an unstructured dynamic model of heterotrophic growth is established. 2, preliminary optimization of stages- supplementary food culture is conducted on the basis of the dynamic model. 3, a hybrid neural network model is established on the basis of multiple stages of culture. 4, optimization of stages- supplementary food culture is conducted on the basis of the hybrid neural network model. The invention can enable the highest cell concentration and producing efficiency of chlorella to respectively reach 100g / l (net weight) and over 1.0g / l / h.

Description

technical field [0001] The invention relates to a feeding method in the technical field of bioengineering, in particular to a feeding optimization method for heterotrophic culture of chlorella. Background technique [0002] Chlorella is a single-cell microalgae, rich in protein, carotenoids, polysaccharides, vitamins and chlorella growth factors and other nutrients and active ingredients, with anti-oxidation, anti-tumor, improving immunity and preventing heart disease. Physiological functions such as vascular diseases. One of the challenges in large-scale cultivation of Chlorella is how to efficiently produce Chlorella cells, which is difficult to achieve in traditional autotrophic cultivation. Heterotrophic culture has attracted the attention of scholars at home and abroad because of its higher cell density and production efficiency of Chlorella, and is considered to be one of the most promising and hopeful production methods. Because higher glucose concentration has inhi...

AI Technical Summary

Problems solved by technology

The main disadvantages of this process are: (1) adopt intermittent feeding, which causes large fluctuations in substrate concentration and affects the growth of Chlorella; (2) the determination of feeding strategy depends on frequent sampling and experience, Lack of simple and effective control methods; (3) the cell density of Chlorella is still quite different from the batch-fed culture level (cell density is usually greater than 100g / l) of yeast and Escherichia coli, etc.

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