Extraction of active ingredient such as betulinic acid from common camptotheca fruit with clean method

Abstract

The invention discloses a method for extracting betulinic acid, hydroxycamptothecin and camptothecin from a camplotheca acumiate fruit in a clean way, which takes the camplotheca acumiate fruit as raw materials and extracts active ingredients by using the methods of polyamide column chromatography and ethanol elution. The device required by the method is simple and the whole extracting and separating process is environment friendly by using ethanol to elute, the whole process needs only 60 percent of the ethanol, the consumption of a desorption agent is few, the materials are obvious to segment, the method is simple and easy, the ethanol can be recycled, thus greatly lowering the cost and being applicable to relatively larger scale preparation, having relatively higher application value and excellent social benefits and values, and being significant in making full use of the camplotheca acumiate fruit of China.

Description

technical field [0001] The invention relates to a method for extracting effective plant components, in particular to a method for extracting betulinic acid, hydroxycamptothecin and camptothecin from philodendron. Background technique [0002] Betulinic acid is a kind of triterpenoid compound, which has the effect of anti-tumor cell growth, and there are many new mechanisms of action in anti-AIDS, which makes betulinic acid a promising drug and has attracted widespread attention. focus on. Franoise et al. found that some new X-aminoalkanoic acid derivatives of betulinic acid have anti-AIDS effects, which can inhibit HIV-1 in CEM4 and MT-4 cells, and will not affect HIV-1 The activity of reverse transcriptase or protease, which is a pharmacological activity that is not found in other anti-AIDS drugs, is therefore of great significance. [0003] The preparation method of betulinic acid mainly contains three ways at present: 1, direct extraction method. Among them, Carcache B...

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Problems solved by technology

[0005] In summary, most of the reagents used in the current method for extracting betulinic acid and camptothecin are highly toxic drugs, such as chloroform, petroleum ether, methanol, etc. The process is cumbersome, the equipment requirements are high, and the production cost is high. high

And the technology that adopts clean method to extract betulinic acid from philodendron has no report, especially the method that can separate and extract camptothecin, hydroxycamptothecin and other active ingredients at the same time, if this method can be realized, for white betulin The research on camptothecin, camptothecin and hydroxycamptothecin is of great significance, and it is also of great significance to make full use of the resources of camptothecin in our country

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