Extraction of active ingredient such as betulinic acid from common camptotheca fruit with clean method

A technology for betulinic acid and active ingredients, which is applied in the field of extraction of plant active ingredients, can solve the problems of cumbersome process, high production cost and high equipment requirements, and achieves the effects of obvious material segmentation, simple equipment and less dosage.

Inactive Publication Date: 2008-07-16
ZHONGKAI UNIV OF AGRI & ENG
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Problems solved by technology

[0005] In summary, most of the reagents used in the current method for extracting betulinic acid and camptothecin are highly toxic drugs, such as chloroform, petroleum ether, methanol, etc. The process is cumbersome, the equipment requirements are high, and the production cost is high. high
And the technology that adopts clean method to extract betulinic acid from philodendron has no

Method used

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  • Extraction of active ingredient such as betulinic acid from common camptotheca fruit with clean method
  • Extraction of active ingredient such as betulinic acid from common camptotheca fruit with clean method
  • Extraction of active ingredient such as betulinic acid from common camptotheca fruit with clean method

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Experimental program
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Effect test

Embodiment 1

[0039] 1. Materials

[0040] The dry seeds of Campylodon chinensis, the polyamide used in the experiment were provided by Nankai University Chemical Factory and Taizhou Luqiao Sijia Biochemical Plastic Factory, analytically pure and high performance liquid chromatography pure ethanol, methanol and phosphoric acid, and analytically pure triethylamine.

[0041] 2. Experimental equipment

[0042] SHZ-D(III) circulating water vacuum pump, rotary evaporator RE-52AA, UV-1 type three-purpose ultraviolet analyzer, LQC·DECA×P liquid chromatography-mass spectrometer coupled instrument, charged spray ion source; atmospheric pressure chemical ionization source , mass range 50-2000 μ, Thermo Finnign Company (USA), Beijing Analytical Instrument Factory SY-5000 high performance liquid chromatography, C18 column.

[0043] 3. Method

[0044] (1) Weigh 500g octopus fruit, crush it and soak it with industrial ethanol for five days, and then filter to obtain a clarified leachate.

[0045] Pret...

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Abstract

The invention discloses a method for extracting betulinic acid, hydroxycamptothecin and camptothecin from a camplotheca acumiate fruit in a clean way, which takes the camplotheca acumiate fruit as raw materials and extracts active ingredients by using the methods of polyamide column chromatography and ethanol elution. The device required by the method is simple and the whole extracting and separating process is environment friendly by using ethanol to elute, the whole process needs only 60 percent of the ethanol, the consumption of a desorption agent is few, the materials are obvious to segment, the method is simple and easy, the ethanol can be recycled, thus greatly lowering the cost and being applicable to relatively larger scale preparation, having relatively higher application value and excellent social benefits and values, and being significant in making full use of the camplotheca acumiate fruit of China.

Description

technical field [0001] The invention relates to a method for extracting effective plant components, in particular to a method for extracting betulinic acid, hydroxycamptothecin and camptothecin from philodendron. Background technique [0002] Betulinic acid is a kind of triterpenoid compound, which has the effect of anti-tumor cell growth, and there are many new mechanisms of action in anti-AIDS, which makes betulinic acid a promising drug and has attracted widespread attention. focus on. Franoise et al. found that some new X-aminoalkanoic acid derivatives of betulinic acid have anti-AIDS effects, which can inhibit HIV-1 in CEM4 and MT-4 cells, and will not affect HIV-1 The activity of reverse transcriptase or protease, which is a pharmacological activity that is not found in other anti-AIDS drugs, is therefore of great significance. [0003] The preparation method of betulinic acid mainly contains three ways at present: 1, direct extraction method. Among them, Carcache B...

Claims

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Application Information

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IPC IPC(8): C07D491/22C07J53/00A61K36/185
Inventor 刘展眉何洁萍钟雪莲
Owner ZHONGKAI UNIV OF AGRI & ENG
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