Multiple PCR detecting reagent case for diagnosis of oesophagostomiasis in pig

Abstract

The invention discloses a multiple PCR detection kit which can be used in the diagnosis of the swine esophagostomiasis. The detection kit comprises DNA lysis solution, PCR reaction solution, as well as the DNA positive control of toothed Oesophagostomum and the positive control of four-ratched Oesophagostomum. By using the ITS repeat DNA fragments of swine toothed Oesophagostomum and four-ratchedOesophagostomum as genetic markers, the invention builds up a fast, specific and sensitive multiple PCR methods which can be used for diagnosing swine esophagostomiasis. With simple and programmed operation and objective result judgments, the invention can be used for diagnosing swine esophagostomiasis, for investigating epidemiology, and for identifying and diagnosing toothed Oesophagostomum andfour-ratched Oesophagostomum.

Description

Multiplex PCR detection kit for the diagnosis of porcine esophageal stoma nematode disease technical field The invention relates to the technical field of diagnosis and detection of porcine oesophagus nematode disease, in particular to a PCR detection kit and a detection method for porcine dentate oesophagus nematode and tetraacanthus oesophagus nematode. Background technique Oesophagostomum nematode disease is caused by a variety of nematodes of the genus Oesophagostomum that parasitize the intestinal tract of animals. Because some species of larvae form nodular lesions in the intestinal wall, it is called nodularia. cause colitis. At present, the disease is ubiquitous in cattle, sheep, and pigs in various parts of our country, and its distribution is extremely wide, causing large economic losses to animal husbandry production. Although esophagostoma is usually only found in ruminants, pigs and monkeys, it has also been reported to infect humans, especially in certain pa...

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Problems solved by technology

However, no convenient and specific detection kits have been developed yet.

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