Method for preserving stropharia rugoso-annulata strain

Abstract

The invention discloses a preserving method of large ball-cover mushroom strain comprising a) configuring directing domestication plating medium, b) preparing non-freezing, non-negative pressure mycelium, c) freezing and drying the mycelium in vacuum: putting the ampoule pipe into refrigerating drier to freeze to -40 DEG C at the speed of 2 DEG C per minute, pre-freezing in constant temperature for 1.5 hours and then opening the vacuum pump, after the vacuum degree achieving 22.5 Pa within 15 minutes, the temperature of the isolation plate rising 5 DEG C and the vacuum degree reducing 5Pa per30 minutes until the temperature of the isolation plate rising to 24 DEG C and the vacuum degree reducing to 0.8 to.

Description

technical field [0001] The invention relates to a method for preserving edible fungus strains, in particular to a method for preserving strains of Stropharia stropharcoides. Background technique [0002] As an important biological resource, strains should be preserved in a timely manner in order to keep their excellent properties unchanged. At present, the preservation methods of edible fungus strains mainly include liquid nitrogen cryopreservation method, inclined plane regular transplantation method, liquid paraffin preservation method, Sand tube preservation method and natural matrix preservation method, etc. Among them, liquid nitrogen cryopreservation method is a relatively advanced method at present, but the cost is high, and foreign preservation institutions mostly use this method; in my country, the edible fungi currently in production or research Bacterial strains are still mainly preserved in test tubes or bottles. The main disadvantages are: short storage time, poor...

AI Technical Summary

Problems solved by technology

[0002] As an important biological resource, strains should be preserved in a timely manner in order to keep their excellent properties unchanged. At present, the preservation methods of edible fungus strains mainly include liquid nitrogen cryopreservation method, inclined plane regular transplantation method, liquid paraffin preservation method, Sand tube preservation method and natural matrix preservation method, etc. Among them, liquid nitrogen cryopreservation method is a relatively advanced method at present, but the cost is high, and foreign preservation institutions mostly use this method; in my country, the edible fungi currently in production or research Bacterial strains are still mainly preserved in test tubes or bottles. The main disadvantages are: short storage time, poor strain stability, easy to mutate or degrade, high cost, and inconvenient to carry.

[0003] The existing edible fungus mycelium live-dried strain and production method thereof, use mycelium as material and utilize vacuum freeze-drying technology freeze-drying to obtain edible fungus live-dried mycelium strain product, but practice shows that mycelium is not suitable for freezing Dry