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Preservation method for mushroom strain

A technology for the preservation of strains and mushrooms, which is applied in the directions of microorganism-based methods, preservation of microorganisms, biochemical equipment and methods, etc., can solve the problems of operating contaminated strains, short storage time, inconvenient carrying, etc., and achieves improved low-temperature freezing resistance. properties, slowing down the loss of water, and wide application

Inactive Publication Date: 2012-07-18
CHANGSHU FANGTA COATING CHEM
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AI Technical Summary

Problems solved by technology

[0002] As an important biological resource, strains should be preserved in a timely manner in order to keep their excellent properties unchanged. At present, the preservation methods of food strains mainly include liquid nitrogen cryopreservation, inclined plane regular transplantation, liquid paraffin method, sand Soil tube preservation method and natural substrate treasure method, etc. Among them, liquid nitrogen cryopreservation method is a relatively advanced method at present, but the cost is high, and foreign preservation institutions mostly use this method; in my country, the edible mushrooms currently produced or researched Bacterial strains are still mainly preserved in test tubes or bottles. The main disadvantages are: short storage time, poor stability of strains, easy to mutate or degrade, high cost, and inconvenient to carry.
If the storage period is too short, due to frequent disturbances and changes in temperature and light, operational pollution and strain degradation often occur, the strain stability is not good, and it is inconvenient to carry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) First prepare the culture medium, its composition formula includes: 20% wheat extract, 31% wood flour, 2% yeast extract, 0.8% sucrose, 2% lactose, buffer (calcium sulfate is used in this example) 0.5%, calcium chloride 0.7%, magnesium sulfate 1.2%, agar 1.2%, feed water 41%. The pH of the prepared solution is 6.8. Make a sterile plate, and when the medium is solidified, lay a layer of porous paper on the surface of the medium;

[0021] (2) Then increase the amount of filler to reduce the contact surface with the air; when the culture medium is cooled to 24°C, inoculate the mushroom strains on the carrier, cultivate in a sterile incubator at 22°C for 100 hours, and then put the carrier together with the strains Take it off together, and cut it according to the size of the bottom of the test tube to the corresponding size, place it in the test tube, compact it slightly, add a cotton plug, sterilize by high-pressure steam at 128°C for 45 minutes, and move the containe...

Embodiment 2

[0027] The preparation medium of this embodiment has a composition formula: 25% of wheat extract, 30% of wood flour, 1.6% of yeast extract, 1% of sucrose, 1.5% of lactose, buffer (calcium carbonate used in this embodiment) 0.8 %, calcium chloride 0.7%, magnesium sulfate 1.3%, agar 1.9%, feed water 44%, pH 6.5. , Calcium chloride 0.7%, magnesium sulfate 1.2%, agar 1.2%, feed water 41%.

[0028] The rest of the operation steps in this embodiment are the same as those in Embodiment 1, and will not be repeated here.

Embodiment 3

[0030] Preservation method of the present embodiment, its steps are:

[0031] (1) Medium preparation, its composition formula includes 30% of wheat extract, 15% of wood flour, 2.8% of yeast extract, 1.2% of sucrose, 2.5% of lactose, 0.6% of buffer (calcium carbonate or calcium sulfate), chloride Calcium 0.9%, magnesium sulfate 1%, agar 1.2%, feed water 37%, pH 6.7. Among them, the ratio of material to water is 1:1. Then make a sterile plate, and when the culture medium is solidified, lay a layer of porous paper on the surface of the culture medium.

[0032] (2) Increase the amount of filler to reduce the contact surface with the air; when the culture medium is cooled to 22°C, inoculate the mushroom strains on the carrier, cultivate in a sterile incubator at 22°C for 100 hours, and then put the carrier together with the strains And take it off, and cut it according to the size of the bottom of the test tube to the corresponding size, place it in the test tube, compact it slig...

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Abstract

The invention discloses a preservation method for mushroom strain. The preservation method comprises the following steps of: (1) preparing a culture medium; (2) increasing volume of filler to reduce contact surface with air; (3) freeze-drying under vacuum; (4) replacing a pipe plug; and (5) packaging and controlling temperature, performing double protection of the strain with a protection bag and a protection box, and placing in a constant temperature refrigerator of 5+ / -1 DEG C for cold preservation to prevent the strain from inactivating easily. According to the preservation method, the performances such as low-temperature cold resistance, negative pressure resistance and rehydration survival rate are improved; a freeze pipe is prepared for storage; hyphae growing into the interior are enabled to survive for long time; gaps are filled through wood flour, so that on one hand, the wood nutrient can be increased, and on the other hand, the dissipation of moisture is reduced; the contact surface with the air is only as large as the internal diameter of a test pipe; and in addition to the special test pipe plug, the water loss rate of the culture medium can be kept less than 30 percent for 3 years. The preservation method has the advantages of low cost, easiness for operation, stability, reliability, long preservation period, high survival rate and wide application range and is worthy of being promoted and used.

Description

technical field [0001] The invention relates to a method for preserving food strains, in particular to a preservation method for mushroom strains. Background technique [0002] As an important biological resource, strains should be preserved in a timely manner in order to keep their excellent properties unchanged. At present, the preservation methods of food strains mainly include liquid nitrogen cryopreservation, inclined plane regular transplantation, liquid paraffin method, sand Soil tube preservation method and natural substrate treasure method, etc. Among them, liquid nitrogen cryopreservation method is a relatively advanced method at present, but the cost is high, and foreign preservation institutions mostly use this method; in my country, the edible mushrooms currently produced or researched Bacterial strains are still mainly preserved in test tubes or bottles. The main disadvantages are: short storage time, poor strain stability, easy variation or degradation, high cos...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12R1/645
Inventor 钱建良
Owner CHANGSHU FANGTA COATING CHEM
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