Crop root exudate collecting method

Abstract

A crop root secretion collecting device and a collecting method thereof relate to a root secretion collecting device and a collecting method thereof. The collecting method solves the problems with the traditional collecting method, including high workload, big error and low precision. The lower part of a PVC culture pan (1) of the collecting device communicates with a drainage tube (4), the otherend of the drainage tube (4) communicates with a collecting bottle (7), and a valve (5) is arranged on the drainage tube (4). The method comprises the following steps: (1) adopting the crop root secretion collecting device; (2) preparing extract; (3) culturing crops in the PVC culture pan (1); (4) pre-washing culture soil by using distilled water; (5) adding the extract to the culture pan (1) forculturing; and (6) opening the valve (5) to allow the liquid in the PVC culture pan (1) to flow into the collecting bottle (7) through the drainage tube (4), and obtaining the liquid of the crop rootsecretion. Compared with the conventional culture method, the collecting method increases the efficiency by 1.5 times and the precision by 10%.

Description

technical field [0001] The present invention relates to a collection method. Background technique [0002] At present, the simulation devices used by people to collect crop root exudates are relatively rough, and the collection conditions are quite different from the actual growth conditions of the crops, resulting in large errors and low precision. The traditional method generally uses to wash the crop root system with water and put it directly in the collection liquid. This collection method is difficult to accurately meet the accuracy requirements of the test and increases the workload. There are many interference factors and large errors in the collection process, which makes some Smaller differences cannot be detected, some are ignored, and the detected data is inaccurate. Contents of the invention [0003] In order to solve the traditional method, the root system of the crops is washed with water and directly placed in the collection liquid. This collection method i...

AI Technical Summary

Problems solved by technology

[0003] In order to solve the traditional method, the present invention washes the crop roots with water and directly puts them in the collection liquid. This collection method is difficult to accurately meet the test requirements, increases the workload, and has many interference factors, large errors, and low precision during the collection process. For small differences that are difficult to detect and some problems that are neglected, a method for collecting crop root exudates is provided. The specific technical solution to solve the above problems is as follows:

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