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Magnetic biological adsorption agent and preparation method thereof

A biosorbent and magnetic technology, applied in chemical instruments and methods, adsorbed water/sewage treatment, other chemical processes, etc., can solve the problems of difficult solid-liquid separation, long action time, low reaction efficiency, etc., to broaden the selection range. , The effect of improving the bearing capacity and simplifying the processing process

Inactive Publication Date: 2010-08-25
HUNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the currently reported biosorbents are free microorganisms, and their application is greatly limited due to problems such as long action time, low reaction efficiency, and difficulty in solid-liquid separation.

Method used

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  • Magnetic biological adsorption agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1. Preparation of Phanerochaete chrysosporium bacterial powder

[0020] Scrape the purchased Phanerochaete chrysosporium (CCTCC AF96007) from the preserved slant medium, and inoculate it into the potato glucose medium, fill each 500mL Erlenmeyer flask with 200mL of liquid medium, and cultivate at a temperature of 30-37°C After vibrating and cultivating at 150-180rpm for 5 days, the bacteria balls were filtered out, washed repeatedly with deionized water, collected and freeze-dried, ground and pulverized through a 180-mesh sieve to obtain bacteria powder. The potato glucose medium consists of: 40g of potatoes, 4g of glucose, and 200mL of water.

[0021] 2. Preparation of magnetic nanoparticles

[0022] (1) Prepare 50mL of 0.4mol / L hydrochloric acid, take 38mL in a beaker, fill with nitrogen for 10min; weigh 8.5g of FeCl 3 ·6H 2 O and 3g FeCl 2 4H 2 O, dissolved in 0.4mol / L hydrochloric acid filled with nitrogen;

[0023] (2) Prepare 375mL of 0.7mol / L ammonia water,...

Embodiment 2

[0031] 1. The preparation of Phanerochaete chrysosporium bacterial powder is the same as in Example 1.

[0032] 2. The preparation of magnetic nanoparticles is the same as in Example 2.

[0033] 3. Immobilization of magnetic nanoparticles and Phanerochaete chrysosporium powder

[0034] Mix the Phanerochaete chrysosporium bacteria powder prepared in the above step 1 and the magnetic nanoparticles prepared in step 2, the mass ratio of Phanerochaete chrysosporium bacteria powder and magnetic nanoparticles is 1: 1.25, and 9g magnetic Add the mixture of nanoparticles and Phanerochaete chrysosporium powder into 40 mL of sodium alginate solution with a mass concentration of 2%, and stir evenly; transfer it dropwise into 200 mL of 0.2 mol / L CaCl with a syringe 2solution, placed at room temperature for 24 hours, then washed several times with sterile water and 0.05mol / L sodium succinate buffer solution (pH4.5), dried, and the immobilization process was completed to obtain uniform and ...

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Abstract

The invention discloses a magnetic biological sorbent. The sorbent uses calcium alginate as a carrier, and is embedded with phanerochaete chrysosporium powder and magnetic nanometer particles, wherein, the mass ratio of the phanerochaete chrysosporium powder to the magnetic nanometer particles to the calcium alginate is 1 to (0.5-1.25) to (8-10). The invention also discloses a method for preparing the magnetic biological sorbent. The method comprises the following steps of: adding a mixture of the magnetic nanometer particles and the phanerochaete chrysosporium powder into a 1 to 2 mass concentration calcium alginate solution with 1 to 2 mass concentration, the dosage of the mixture in the solution being between 0.125 and 0.225g / mL, stirring evenly, and obtaining the magnetic biological sorbent through an immobilization process. The magnetic biological sorbent has the advantages of high adsorption efficiency, good selectivity, low cost, simple preparation process and treatment processand so on, and can be effectively applied to the treatment of lead metal in industrial wastewater.

Description

technical field [0001] The invention relates to a magnetic biosorbent, and also relates to a preparation method of the magnetic biosorbent. Background technique [0002] The harm of heavy metals in the environment has been paid more and more attention to by human beings. Among them, lead is a serious environmental poison and neurotoxicity. Once poisoned, it will cause varying degrees of poisoning to various systems of the human body, especially affecting infants and children's intellectual development and brain functions such as learning and memory. Therefore, the treatment of lead in the environment is not only related to the healthy growth of children, but also affects the intelligence level of the next generation in China, the quality of the population and the long-term competitiveness of the country. [0003] Lead-containing wastewater mainly comes from battery production plants, electroplating plants and smelters. For the treatment of lead-containing wastewater, the t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J20/22B01J20/30C02F1/28C02F1/62
Inventor 胡霜曾光明黄丹莲冯冲凌曾炜苏峰峰
Owner HUNAN UNIV
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