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Application of liquid chromatogram and electric atomizing series connection mass spectrum combination in tobacco production

A technology of tandem mass spectrometry and liquid chromatography, which is used in the application field of liquid chromatography and electrospray tandem mass spectrometry in tobacco production

Active Publication Date: 2009-01-14
CHINA TOBACCO GUANGDONG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the analysis of amino acids in tobacco samples by liquid chromatography and electrospray tandem mass spectrometry.

Method used

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  • Application of liquid chromatogram and electric atomizing series connection mass spectrum combination in tobacco production
  • Application of liquid chromatogram and electric atomizing series connection mass spectrum combination in tobacco production
  • Application of liquid chromatogram and electric atomizing series connection mass spectrum combination in tobacco production

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1 Method for analyzing amino acids by liquid chromatography and electrospray tandem mass spectrometry

[0018] In this example, in the amino acid analysis and detection of tobacco and its products, the method of liquid chromatography analysis-electrospray tandem mass spectrometry analysis is adopted. The summary and limitation of liquid chromatography analysis conditions during the experiment are also applicable to individual mass spectrometry analyze.

[0019] 1. Instruments, materials and reagents

[0020] LC-MS LTQ Liquid Chromatography-Ion Trap Mass Spectrometer from American Thermo Company, equipped with autosampler, quaternary gradient mass spectrometer pump, LTQ ion trap mass spectrometer charged spray ionization source (ESI), Xcalibur 1.4 SRl system control software; Italy Claind N2LCMS Nitrogen generator; French Millipore ElementA10 pure water machine; American Cole-Parmer 8892 ultrasonic cleaner; Sartorious CP225D electronic balance (Max 220g, d=0.01m...

Embodiment 2

[0067] Example 2 Application of Liquid Chromatography and Electrospray Tandem Mass Spectrometry in Tobacco Production

[0068] The established method was used to test a variety of tobacco samples, and the results are shown in Table 6 and Table 7.

[0069] The content of Cys in tobacco was very low and could not be detected in all samples. The total amount of amino acids in flue-cured tobacco leaves generally accounts for between 0.3% and 0.96% of the tobacco content, and the amino acid content in Burley tobacco is relatively high, generally 2 to 3 times that of flue-cured tobacco leaves. The contents of Asp, Thr, Glu, Gaba, Val, Lys, Ile, Arg, Leu, Phe and Asn in Burley tobacco were significantly higher, and His content was relatively higher. Burley tobacco had the highest Asn content, more than double its Pro content. Non-burley tobacco generally has the highest Pro content, but there are a few samples with Ash higher than Pro, such as samples Hunan 03 and American 01, etc....

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Abstract

The invention discloses the coupled application of a liquid chromatogram and an electrospray tandem mass spectrometry in the tobacco production, determines the classes of a cigarette or a single cigarette material according to an analyzed result that is obtained through quick and accurate amino acid content measurement of tobaccos and tobacco products, evaluates the homogenization situation of the cigarette by using an amino acid analyzing result according to the theoretical base that the cigarettes with the same brand and the same model have basically the same content of various amino acids, and consequently monitors the consistency of cigarette quality.

Description

technical field [0001] The invention belongs to the technical field of chemical analysis and detection, and in particular relates to the application of liquid chromatography and electrospray tandem mass spectrometry in tobacco production. Background technique [0002] Amino acids are an important part of tobacco leaves, and are tobacco components closely related to smoke aroma, cigarette quality and brand characteristics. Amino acids participate in the non-enzymatic brown reaction in tobacco leaves, namely the Maillard reaction, which can produce a variety of important Aroma ingredients. Therefore, the determination of amino acids in tobacco is of great significance to formula development, process production and quality control. [0003] In recent years, with the development of electrospray ionization (ESI) and tandem mass spectrometry (MS / MS), liquid chromatography coupled with electrospray tandem mass spectrometry (LC-ESI-MS / MS) has been widely used in the analysis of com...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 黄翼飞
Owner CHINA TOBACCO GUANGDONG IND