Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance

A technology for resisting stripe leaf blight and protein, which is applied in botany equipment and methods, biochemical equipment and methods, plant products, etc., can solve the problems of poor resistance and low yield of low glutenin rice varieties, shorten the life span, improve Breeding efficiency, good quality effect

Inactive Publication Date: 2009-01-21
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Technical problem The object of the present invention is to provide a kind of breeding method of the rice variety of polymerization low glutenin and resistance to stripe leaf blight, cultivate the production equivalent with current main cultivar, and absorbable protein (comprising albumin-globulin and glutenin) ) i

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  • Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance
  • Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance
  • Method for breeding rice vairety with polymerized low-glutelin and rice stripe-resistance

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[0038] 1. Taking the low-glutenin rice variety W3660 as the female parent and Zhendao 88 as the male parent, the hybrid offspring were self-crossed. From F1, the whole protein of the offspring seeds was subjected to sodium dodecyl sulfonate denaturation-polyacrylamide coagulation. Gel electrophoresis (SDS-PAGE) analysis showed that low glutenin and high prolamin traits were always found together; F 1 The seeds were all characterized by low gluten and high prolamin content ( figure 2 ); F 2 The ratio of low gluten and normal protein traits in seeds is about 3:1 ( image 3 ); measure F 3 Seed protein, the segregation ratio of its low-gluten homozygous, heterozygous and normal type conforms to F 2 Plant genotype 1:2:1 ( Figure 4). indicate that the low gluten and high prolamin traits are controlled by a single dominant gene ( image 3 ), and can be stably passed on to offspring.

[0039] 2. Crossbreed with W3660 as the female parent and Zhendao 88 as the male parent. 2 ...

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Abstract

The invention discloses a breeding method of a rice species combining the properties of low glutelin and rice stripe disease resistance, which pertains to the field of rice breeding. W3660 is taken as a female parent and Zhendao 88 is taken as a male parent for hybridization; and since F2 generation, a single plant with a main ear having 12 grains, the holoprotein SDS-PAGE protein band pattern of which is in line with W3660 holoprotein band pattern, is selected for planting; WW1 and R15 marks are used for assisting the selection; by combining the field inoculation and manual inoculation certification of the plants with rice stripe disease resistance, a plant strain which has rice stripe disease resistance and is characterized in that the SSR fingerprint has a low glutelin characteristic band 237bp marked by WW1 and a rice stripe disease resistance characteristic band 363bp marked by R15, and the growth duration, plant height, and shape and characteristics of the plant leaves are identical with that of the Zhendao 88 is selected and named as W1721. After determination, the new rice variety W1721 has low glutelin content and less than 4% of the absorbable protein (including albumin-globulin and glutelin) content and has rice stripe disease resistance.

Description

1. Technical field [0001] The invention relates to a breeding method for rice varieties with low glutenin polymerization and resistance to stripe leaf blight, which belongs to the polymerization breeding method of new rice varieties with low gluten protein and resistance to stripe leaf blight, and is specially used for people with kidney disease and diabetes to eat rice and Breeding of varieties resistant to stripe leaf blight. 2. Background technology [0002] In recent years, due to changes in the living habits and ecological environment of urban and rural residents in my country, the number of patients with kidney disease and diabetes in China has been increasing. According to statistics, the total number of diabetic patients in China has exceeded 100 million in recent years, of which nearly 6 million are due to diabetes He was hospitalized for kidney disease. Patients with kidney disease and diabetes (combined with kidney disease) have protein metabolism dysfunction. Dur...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H5/00C12Q1/68
Inventor 万建民江玲刘世家王益华张迎信刘喜陈亮明赵志刚刘玲珑张文伟翟虎渠
Owner NANJING AGRICULTURAL UNIVERSITY
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