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Plant-optimized polynucleotides encoding approximately 15 kDa and approximately 45 kda pesticidal proteins

A polynucleotide and protein technology, applied in the direction of pesticides, plant genetic improvement, botany equipment and methods, etc.

Inactive Publication Date: 2009-01-21
MYCOGEN CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the discovery of new B.t. isolates and the discovery of new uses for known B.t. isolates remains an empirical, unforeseen art

Method used

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  • Plant-optimized polynucleotides encoding approximately 15 kDa and approximately 45 kda pesticidal proteins

Examples

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Embodiment 1

[0048] Example 1 - Insertion of toxin genes into plants

[0049] One aspect of the invention is the transformation of plants with a subject polynucleotide sequence encoding an insecticidal toxin. The transformed plants are resistant to attack by the target pest. The genes of the invention are optimized for use in plants.

[0050] Obviously, a promoter region capable of expressing the gene in plants is required. Thus, for expression in planta, the DNA of the invention is under the control of a suitable promoter region. Methods for obtaining expression in plants using such constructs are known in the art. A preferred promoter region for expression of both the 15kDa and 45kDa transgenes is the maize ubiquitin promoter plus maize exon 1 and maize intron 1 (Christensen, A.H. et al., 1992 Plant Mol. Biol. 18:675- 689). A preferred transcription terminator for both transgenes is the potato proteinase inhibitor II (Pin II) terminator (An, G. et al., 1989 Plant Cell 1:115-22). ...

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Abstract

The subject invention provides new, plant-optimized polynucleotide sequences that encode pesticidal toxins. The polynucleotide sequences of the subject invention have certain modifications, compared to wild-type sequences, for example, that make them particularly well-suited for optimized expression in plants. Using the polynucleotide sequences described herein, the transformation of plants can be accomplished, using techniques known to those skilled in the art, in order to confer pest resistance upon said plants. In preferred embodiments, the subject invention provides plant-optimized polynucleotide sequences which encode approximately 15 kDa and approximately 45 kDa pesticidal proteins.

Description

[0001] This application is a divisional application, the application number of its parent application is 99812497.4, the application date is October 21, 1999, and the title of the invention is "a plant-optimized polynucleotide encoding an insecticidal protein of about 15 kDa". Background of the invention [0002] Insects and other pests cost farmers hundreds of millions of dollars in annual crop losses and the cost of controlling these pests. Losses to the agricultural production environment caused by insect pests include reduced crop yields, reduced crop quality, and increased harvest costs. [0003] Chemical pesticides have provided effective methods of controlling pests; however, the public has become concerned about the amount of chemical residues that may be found in food, groundwater, and the environment. Consequently, synthetic chemical pesticides are increasingly being scrutinized, particularly for their potential toxic environmental consequences. Synthetic chemical p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/32C12N15/82C12N5/10C07K14/325A01P7/04
Inventor G·A·卡迪尼奥S·J·斯蒂尔曼K·E·纳瓦
Owner MYCOGEN CORP