QTL mapping range of bruchid resistance in mung bean

A technology for resisting bean weevil and mung bean weevil, which is applied to the determination/inspection of microorganisms, plant genetic improvement, botany equipment and methods, etc.

Inactive Publication Date: 2009-02-04
INST OF CROP SCI CHINESE ACAD OF AGRI SCI +1
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  • Abstract
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Problems solved by technology

At present, there is no report on the analysis of the QTL of mung bean resistance to L.

Method used

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  • QTL mapping range of bruchid resistance in mung bean
  • QTL mapping range of bruchid resistance in mung bean

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] Embodiment: Indoor artificial inoculation identification of anti-mung bean weevil

[0016] In order to preserve the germplasm, under the condition that the seed quality and population structure in the family are still random populations, two replicates of 210 and 217 F 9 RIL was used as the test material, and the indoor artificial inoculation method selected by the national scientific and technological research project was adopted, and the inoculation was carried out in July 2005 and July 2006 respectively. In 2006, 210 copies of F9RIL were planted in Beijing and Nanjing in 2005, respectively, and 72 and 59 F10RIL populations were harvested for inoculation. Zhonglu No. 1 was used as the control for the two-year inoculation, and two repetitions were set. Randomly select 60 healthy seeds from each family and divide them into 2 parts, each with 30 seeds, put them into a small plastic box with a diameter of 6 cm and a height of 1 cm, without a cover, and put them into a la...

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Abstract

The invention provides a linkage group mapping interval STSbr1-VrCS161of mungbean weevil resistance QTL, and the STSbr1 of the interval can be used as the molecular marker of selecting mungbean weevil resistance materials. The significance of the invention lies in providing a QTL mapping interval and providing a novel economic effective molecular breeding approach for auxiliary breeding of mungbean weevil resistance molecular markers and accelerating the breeding process to reduce the harm of the mungbean weevils.

Description

technical field [0001] The invention belongs to the field of molecular breeding, and in particular relates to a QTL mapping interval for mung bean resistance to Elephant maize. Background technique [0002] Mung bean [Vigna radiate (L.) wiclzek] is the main edible bean crop in my country. However, bean weevil (Genus Callosobruchus) is the most serious worldwide storage pest to mung bean and other Vigna crops, mainly including mung bean weevil (Callosobrnchus. Elephants (Callosobrnchus. phaseoli G.), among which the mung bean and four-striped bean are the most harmful. Bean weevil damage will reduce mung bean yield, quality and germination rate. In one life cycle of mung beans, 30% to 56% of the yield can be lost due to bean weevil damage. If the re-infestation is more harmful, even the whole warehouse of mung beans will be damaged. Chemical insecticide control of bean weevil will inevitably increase the production cost of mung bean, pollute the ecological environment, and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/04
Inventor 程须珍刘春吉梅丽王素华王丽侠刘长友孙蕾徐宁
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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