QTL mapping range of bruchid resistance in mung bean
A technology for resisting bean weevil and mung bean weevil, which is applied to the determination/inspection of microorganisms, plant genetic improvement, botany equipment and methods, etc.
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[0015] Embodiment: Indoor artificial inoculation identification of anti-mung bean weevil
[0016] In order to preserve the germplasm, under the condition that the seed quality and population structure in the family are still random populations, two replicates of 210 and 217 F 9 RIL was used as the test material, and the indoor artificial inoculation method selected by the national scientific and technological research project was adopted, and the inoculation was carried out in July 2005 and July 2006 respectively. In 2006, 210 copies of F9RIL were planted in Beijing and Nanjing in 2005, respectively, and 72 and 59 F10RIL populations were harvested for inoculation. Zhonglu No. 1 was used as the control for the two-year inoculation, and two repetitions were set. Randomly select 60 healthy seeds from each family and divide them into 2 parts, each with 30 seeds, put them into a small plastic box with a diameter of 6 cm and a height of 1 cm, without a cover, and put them into a la...
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