Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array

A nanorod array, early diagnosis technology, applied in the field of biomedical detection, can solve the problems of intricate clinical practice, and achieve the effect of enhancing the intensity of photoluminescence

Inactive Publication Date: 2009-02-04
SICHUAN UNIV
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  • Application Information

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Problems solved by technology

AFP is a good indicator for monitoring liver cancer, especially for the early diagnosis of small liver cancer. However, clinical practice is complex and ever-changing. There are still

Method used

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  • Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array
  • Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array
  • Hepatocarcinoma early diagnosis kit based on ZnO nano-bar array

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] (1) Add 1.5ml of fresh serum sample to the electrophoresis tank and dilute to 3.5ml with buffer;

[0021] (2) Carefully insert the detection bottom plate into the sample solution, the liquid level is flush with the lower line of the absorbent paper on the bottom plate; then install the micro electric field, adjust the direction of the electrodes, make the detection bottom plate connect to the positive pole of the power supply, and the opposite electrode is the negative pole of the electric field, and turn on the power supply ;

[0022] (3) Turn off the power after 8 minutes, carefully take out the detection base plate, then rinse the ZnO array detection area of ​​the base plate horizontally for 2-4 times with buffer solution to remove excess adsorbed non-tumor antigen proteins, and finally dry it with hot air;

[0023] (4) Put the detection base plate into the miniature spectrum tester for spectrum detection. The wavelength of the excitation light is 270nm, and the emi...

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Abstract

The present invention provides a ZnO nanorod array detection kit which is used for early diagnosis of the liver cancer. The kit adopts anti-AFP protein, anti-AFP (alpha-fetoprotein) protein, anti-sugar protein 19-9 and anti-sugar protein 125; the kit consists of an improved ZnO nanorod array film which is coated by the four kinds of protein, a bottom plate, a micro field device (with an electrophoresis tank and a pair of electrodes) and buffer solution; the invention also relates to the utilizing and detecting method of the kit. The kit can be used for the diagnosis of benign and malignant primary liver cancer tumors, in particular for the detection of the early clinical primary liver cancer, and can be used for the chemotherapeutic monitoring and the prognostic evaluation after the operation on the liver cancer. The invention has the characteristics of high speed, sensitivity, accuracy, reliability, simple operation, and suitability for the primary health care units and the hospitals of various levels.

Description

technical field [0001] The invention relates to the field of biomedical detection, in particular to the early diagnosis and general survey of detecting liver cancer by using a tumor-specific antibody modified ZnO nanorod array film. Background technique [0002] Liver cancer is one of the common malignant tumors in our country, and it is the second cancer "killer" in our country, which is common in middle-aged men. Because of its high degree of malignancy and rapid disease progression, patients generally have no discomfort in the early stage. Once symptoms appear, it is often in the middle and late stages. Therefore, the treatment is difficult and the curative effect is poor. Generally, the survival time after the onset is only 6 months. It is called "the king of cancer". Liver cancer accounts for 45% of the total death toll in the world, and it poses a great threat to the life and health of our people. It is precisely because most of its clinical findings are in the middl...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N33/543G01N21/63
Inventor 廖晓明黄忠兵尹光福康云清姚亚东
Owner SICHUAN UNIV
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