Microwave extraction method of steroid saponin from puncturevine

Abstract

The invention provides a microwave extraction technology for steroid saponins in caltrops, which comprises the following steps: weighing caltrop sample and placing the caltrop sample into a microwave extraction bottle, adding alcohol in the microwave extraction bottle, placing the microwave extraction bottle in a MAS-II type normal-pressure microwave-assisted synthesis / extraction reaction instrument for extraction, performing decompression filtration to an obtained extract, and then evaporating a solvent on a rotary evaporator to obtain a crude extract of the steroid saponins of the caltrops; adopting a HCl solution to hydrolyze the steroid saponins extracted by microwave, obtaining sapogenins corresponding to the steroid saponins of the caltrops after the hydrolysis, dissolving the saponins with chloroform, and then filtering the saponins for GC-MS detection. The technology adopts a microwave-assisted hot reflux method to extract the steroid saponins of the caltrops, combines a gas chromatography mass spectrometry to detect steroidal sapogenins of the caltrops, ensures that the pretreatment is simple and convenient and the operation is simple by optimizing extraction conditions, has high extraction efficiency, and is environment-friendly and nontoxic.

Description

technical field [0001] The invention belongs to the field of analytical chemistry, and more specifically relates to a microwave extraction technology of steroidal saponins in Tribulus terrestris. Background technique [0002] Chinese medicine is the traditional quintessence of our country, and the research on the active ingredients of Chinese medicine and the modernization of Chinese medicine is a hot spot in the research of Chinese medicine. Tribulus terrestris has a long history of medicinal use in my country, and the research on its chemical constituents began in the 1960s. Tribulus terrestris is rich in steroidal saponins, which can stimulate the secretion of luteinizing hormone in the pituitary gland of the human body, thereby promoting the secretion of testosterone, increasing the level of blood testosterone, increasing muscle strength, and promoting the recovery of physical strength, thereby significantly improving the athletic ability of athletes. However, the compo...

AI Technical Summary

Problems solved by technology

However, the composition of traditional Chinese medicine is complex, limited by pretreatment and detection technology, the separation and extraction of active ingredients of traditional Chinese medicine has always been the bottleneck of Chinese medicine research

At present, the research on the effective components of steroidal saponins from Tribulus terrestris is limited to the separation and extraction of total saponins from Tribulus terrestris, and there is no report on the separation and extraction of a single steroidal saponin, let alone the discussion on its sports nutrition efficacy

Due to the complex matrix of Tribulus terrestris and the wide variety of steroidal saponins, the extraction rate of steroidal saponins from Tribulus terrestris is low by traditional pretreatment methods

Moreover, steroidal saponins are generally difficult to gasify, and the phenomenon of isomerization is serious, so the separation and detection effect of gas phase and liquid chromatography is not ideal.

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