Method for detecting protein by electrochemical activity switch adaptation body beacon
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A beacon detection and electrochemical technology, applied in the field of chemical analysis, can solve the problems of large-capacity sample detection difficulties, low detection sensitivity, high background current, etc., and achieve the effect of simple method, good selectivity, and low background signal
Inactive Publication Date: 2009-03-11
EAST CHINA NORMAL UNIVERSITY
View PDF0 Cites 8 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
The method is mainly to realize the conversion of biomolecules/electrochemical signals by changing the distance between the electrochemically active molecules and the electrode surface [Document: Fan, C. et al. Proc. Natl. Acad. Sci., 2003, 100, 9134- 9137; Wang, J., Li, J., Baca, A.J., Hu, J., Zhou, F., Yan, W., Pang, D.-W., Anal. Chem., 2
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Examples
Experimental program
Comparison scheme
Effect test
Example Embodiment
[0016] Example: Determination of Thrombin Protein in Solution
[0017] Step 1: According to the synthesis of 3.0 O.D. nucleic acid amount, take 90μL 3.3×10 -3 moL -1 Carminic acid solution, add 60mg imidazole, adjust the pH value of the solution to 6.5, after the carboxyl group is activated at room temperature for 30 minutes, in the presence of coupling agents of about 12mg EDC and 27mg NHS, add 3.0O.D. -thrombin aptamer (29mer) nucleic acid aptamer PBS solution (pH = 7.3), the solution volume is 1200 μ L, the mixture is stirred in an ice-water bath for 24 hours, the dialysis bag (molecular weight is 7000) is dialyzed in PBS solution for 48 hours, remove Excess carminic acid not coupled with nucleic acid was purified by HPLC method to prepare 1100 μL concentration of 1.5×10 -5 moL -1 The electrochemically active switch aptamer beacon was stored in a 4°C refrigerator for later use.
[0018] The second step: respectively containing 1.0, 2.0, 4.0, 8.0, 12.0×10 -11 moL -1 In...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention discloses a method for detecting protein by using electrochemical activity switch aptamer beacon, which belongs to the technical field of chemical analysis methods and comprises two steps of the synthesis of the electrochemical activity switch aptamer beacon and the detection of specific protein molecules of a sample. The aptamer beacon has no electrochemical activity, but when combined with target molecules, the aptamer beacon has electrochemical activity due to the change of the stem-loop structure thereof, and generates oxidation peak current which has linear relationship with the concentration of the protein in the solution; therefore, the content of the protein molecules can be calculated. The method takes the electrochemical activity switch aptamer beacon as a detection probe without a reagent and being fixed, has the advantages of good selectivity, sensitivity, simpleness and convenience, and can quickly and directly carry out homogeneous detection on such biomolecules as aptamer with wide linear detection range, thereby particularly being applicable to such humble situations as fields and the like, and detecting the sample with large objective parameter change.
Description
technical field [0001] The invention relates to a method for detecting protein by using an electrochemically active switch aptamer beacon, and belongs to the technical field of chemical analysis methods. Background technique [0002] Molecular beacon technology is the focus of biomolecular probe research. It can: carry out quantitative detection to PCR amplification product and carry out real-time online detection to PCR amplification process (real-time PCR) [document: Tyagi, F.R.Kramer, Nat Biotechnol, 1996,14,303-308; (b) Alsmadi OA, Al-Kayal F, Al-Hamed M, and Meyer BF, BMC Med Genet 2006, 7, 43-49.]; for the quantitative and qualitative detection of genes and for the analysis of gene point mutations and double-stranded DNA Detection [Literature: Du H, Disney MD, MillerBL, Krauss TD, J.Am.Chem.Soc., 2003, 125, 4012-4013; (d) Marras, S.A.E., Kramer, F.R.and Tyagi, S.Genet.Anal .Biomol.E, 1999, 14, 151-156; basic medical research and direct application in clinical medicin...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more