Application of dominant suppressing mutant F427D as anthrax bacillus toxin inhibitor and vaccine

An anthrax toxin and inhibitor technology, applied in bacteria, bacterial peptides, antibacterial drugs, etc., can solve problems such as adverse reactions of the body, and achieve the effect of high biological safety and good immunogenicity
CN101392232BInactive Publication Date: 2012-06-27HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2012-06-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the technical fields of animal bacteriology and zoonosis science, particularly to an application of a dominant inhibitory mutant F427D as an inhibitor of Bacillus anthracis toxin and an application of vaccine thereof. The invention, through cloning of Bacillus anthracis lethal toxin gene, expression and purification of proteins, site-directed saturation mutagenesis of genes and the like, obtains protective antigen (namely the dominant inhibitory mutant F427D) of the Bacillus anthracis, the mutant is successfully cloned into pronucleus expression carrier pGEX-KG and composes recomposed plasmid pGEX-KG-PA (F427D). Escherichia coli containing the recomposed plasmid is nominated as Escherichia coli DH5Alpha / pGEX-KG-PA (F427D) and collected in the China Center for TypeCulture Collection with a collection number of CCTCC-M208158. The invention further discloses the method for preparing the antigen protein, the preparation of anthrax toxin inhibitor and subunit vaccine by using the antigen protein and the application thereof.
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Description

technical field

[0001] The invention relates to the technical fields of animal bacteriology and zoonotic infectious diseases. It specifically relates to the application of a dominant suppressing mutant F427D as an anthracis bacillus toxin inhibitor and a vaccine. Background technique

[0002] Anthrax is an acute, febrile, septic infectious disease caused by Bacillus anthracis. Characterized by sudden hyperthermia and death, cyanosis of visible mucosa, and coal tar-like blood from natural orifices. The OIE lists it as a Category B disease and a nationally notifiable disease. Bacillus anthracis is a long, thick, large bacterium of the aerobic Bacillus genus, without flagella, immobile, and Gram-positive. There are two forms of propagules and spores, and anthrax spores with strong resistance can be formed under sufficient oxygen and appropriate temperature. Because anthrax is easy to produce and prepare, its spore state can exist stably for a long time, it has strong resist...

Claims

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