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Preparation of Dendrobium nobile polysaccharide extract and use there

A technique for extracting polysaccharides and Dendrobium nobile, which is applied in the field of polysaccharide extract preparation from Dendrobium nobile, can solve the problems of rough separation and purification process, unclear material basis, uncontrollable quality, etc. controllable effect

Inactive Publication Date: 2009-04-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on Dendrobium polysaccharides has disadvantages such as unclear material basis, uncontrollable quality, rough separation and purification process, etc., lack of literature and patent reports with high active ingredient content, clear chemical composition, and significant pharmacodynamic activity.
And, also do not see the appearance of the medicine or the pharmaceutical composition about Dendrobium nobile polysaccharide on the market

Method used

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  • Preparation of Dendrobium nobile polysaccharide extract and use there
  • Preparation of Dendrobium nobile polysaccharide extract and use there

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The preparation of embodiment one Dendrobium nobile polysaccharide extract

[0020] (1) Degreasing: take the decoction pieces of Dendrobium nobile, soak them in 8 times the amount of ethanol, reflux twice, filter, and evaporate the organic solvent to get the dregs; 1 hour each time, filter, concentrate the filtrate to 1.0 g of crude drug per ml; (3) alcohol precipitation: add an appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, place in an ice bath for 12 hours, filter, Precipitation and freeze-drying polysaccharide extract crude product I; (4) protein removal: take an appropriate amount of the above polysaccharide extract crude product I and dissolve it in water, add appropriate amount of chloroform and n-butanol, layer after stirring, repeat the operation for the upper layer 4 times, add Appropriate amount of ethanol until the ethanol content is 80%, put it in an ice bath for 12 hours, filter, precipitate and freeze-dry...

Embodiment 2

[0021] The preparation of embodiment two Dendrobium nobile polysaccharide extract

[0022] (1) Degreasing: take the decoction pieces of Dendrobium nobile, soak them with 10 times the amount of 80% ethanol and reflux twice, filter, and evaporate the organic solvent to get the dregs; Extract twice, each time for 1 hour, filter, and concentrate the filtrate to contain 1.5 g of crude drug per ml; (3) Alcohol precipitation: add an appropriate amount of ethanol to the above concentrated solution until the ethanol content is 80%, and place it in an ice bath for 24 hours. Filtrate, precipitate and freeze-dry the polysaccharide extract crude product I; (4) remove protein: take an appropriate amount of the above polysaccharide extract crude product I and dissolve it in water, add appropriate amount of chloroform and n-butanol, stir and layer, and repeat the upper layer for 8 times , add an appropriate amount of ethanol until the ethanol content is 80%, place in an ice bath for 24 hours,...

Embodiment 3

[0023] The preparation of embodiment three Dendrobium nobile polysaccharide extract

[0024] (1) Degreasing: Weigh 4 kg of Dendrobium medicinal material (dried product, block decoction pieces), add 8 times the amount of 80% ethanol to soak for 3 hours (W / V), reflux at 85° C. for 5 hours, twice. Filter, spread the dregs evenly, and evaporate the ethanol; (2) Extraction: evaporate the dregs until there is no alcohol smell, add 4 times the amount of distilled water (W / V), soak for 12 hours, extract in a water bath at 50°C for 2 hours, twice. Suction filtration with four layers of gauze Buchner funnel, collect filtrates, combine, and concentrate to 3000ml by rotary evaporation at 60°C; (3) Alcohol precipitation: add about 9000ml absolute ethanol to the above concentrated solution, adjust to 80% ethanol content with alcohol hydrometer , placed in an ice bath for 24 hours, flocculent precipitation occurred, and was suction-filtered with a four-layer gauze Buchner funnel, precipitate...

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Abstract

The invention provides a preparation method of dendrobium nobile polysaccharide extracts. The dendrobium nobile polysaccharide extracts can be realized through steps of degreasing, extracting, alcohol precipitation, deproteinization, removing monosaccharide and oligosaccharide, gel column purification etc. The content of polysaccharide in the polysaccharide extracts is above 90 percent. The polysaccharide extracts are provided with obvious antioxidant activity and can be applied to the preparation of medicaments or medicament complex used for treating and preventing cardiovascular disease after combined with medicament excipients or carriers allowed by the preparation. The preparation method of the extracts has the advantages of reasonable design and easy operation.

Description

technical field [0001] The invention belongs to a traditional Chinese medicine extract, and in particular relates to a preparation method and application of a dendrobium nobile polysaccharide extract. Background technique [0002] Dendrobium is a commonly used famous and precious Chinese medicinal material, which is the collective name for the fresh or dried stems of various plants of the genus Dendrobium Sw. in the family Orchidaceae. Dendrobium is a large genus in the Orchidaceae, with about 1100 species in the world, and about 76 species in my country. Dendrobium was first recorded in "Shen Nong's Materia Medica", and was listed as a top grade. It has the effects of nourishing yin and clearing heat, nourishing the stomach and promoting body fluid, moistening the lungs and relieving cough. The 2005 edition of "Chinese Pharmacopoeia" recorded three kinds of Dendrobium nobile Lindl., Dendrobium officinale Kimura et Migo and Dendrobium versicolor (D. fimbriatum Hook.). [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P9/00A61P3/10A61P35/00A61P37/04A61K36/8984
Inventor 程翼宇吴斌姚宏
Owner ZHEJIANG UNIV
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