Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method

A technology of Sargassum and Sargassum algae is applied in the directions of botanical equipment and methods, seaweed cultivation, plant cells, etc., and achieves the effects of simple operation, low cost of seedling raising, and low cost.

Inactive Publication Date: 2011-04-06
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method also has its own disadvantages. The main reason is that its cultivation period is relatively long. It takes at least 6-8 months from the cultivation of young leaves to the length that can clamp seedlings.
In addition, the leaf tissue culture method also requires a higher cost

Method used

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  • Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method
  • Sargassum thunbergii seedling quick-propagation method using leader branch segment tillering method

Examples

Experimental program
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Effect test

Embodiment 1

[0028] The sterilized seawater is high-pressure sterilized natural seawater sterilized under the pressure condition of 0.1MPa-0.15MPa for 25-40 minutes;

[0029] Tissue culture experiments were carried out on ultra-clean benches, and all utensils and utensils were autoclaved. Before cutting and culturing, soak the main branch of the algae strain in alcohol with a volume concentration of 75% for 25 to 23 seconds, and then quickly rinse it with a large amount of sterilized seawater for 3 to 4 times. Cut into sections, the length of the section is 2.5-3cm. The sterilized fragments were cultured in jars with a capacity of 20 liters. The medium is: common ES seawater culture medium, which is aerated and cultured, and the culture medium is replaced every 4 days. Other culture conditions are: temperature 15-17°C. The light intensity is 20~30μmol / m 2 per s. The photoperiod is: 14:10h (light:dark). When the cultivation lasts for about a month, the tiller branches of most of the ...

Embodiment 2

[0041] The culture conditions are: temperature 17℃; light intensity 25μmol / m 2 per s; photoperiod 12:12h (light: dark);

[0042] The medium used is: ES medium + BG-11 seawater nutrient solution, that is, the ingredients of BG-11 formula are added to the general ES seawater culture medium;

[0043] BG-11 Formula: H 3 BO 3 2.86μg / l, MnCl.4H2O 1.81μg / l, ZnSO 4 .7H2O0.222μg / l, NaMoO 4 .2H2O 0.39 μg / l, CuSO 4 .5H2O 0.079μg / l, Co(NO3) 2 .6H2O 0.0494μg / l;

[0044] Other culture conditions are with the experimental operation of above-mentioned embodiment 1;

[0045] In Fig. 2, A: main branches of different types of algae strains, 1 and 3 are main branches of type A, and 2 and 4 are main branches of type B; B: regeneration process of main branch fragments of type A strains in the first two weeks , 1 is the fragment when the segment was just cut, 2 is the regeneration status on the 6th day, 3 is the regeneration status on the 10th day, and 4 is the regeneration status on the 1...

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Abstract

The invention relates to the propagation of Sargassum thunbergii, in particular to a method for quickly propagating the Sargassum thunbergii by a method of main branch fragment tillering, which comprises the following steps: 1) alcohol with a volume concentration of 75 percent used by the main branch of the Sargassum thunbergii is adopted for dipping the main branch for 25 to 23 seconds; and thena plurality of disinfected seawater is adopted for quickly washing the main branch for 3 to 4 times; 2) the main branch of the Sargassum thunbergii strain is sheared into fragments with a length of 2to 3cm; the fragments are arranged in a container to culture; universal ES seawater culture liquid is adopted as a medium for ventilation culturing; and when the tillers of the fragments can achieve 1.5 to 3.5cm, the fragments are taken out from the container and the tillers are cut off by a disinfected bistoury; and 3) the cut off tillers are continuously propagated and cultured by adopting the conditions in the step 2) or directly used for the large-scale gripping mariculture. The method is simple to be operated and low in cost; regenerated seedlings can be produced in a circularly; moreover, the method plays the remarkable role of protection on the natural environment.

Description

technical field [0001] The invention relates to the breeding of sargassum, in particular to a method for rapidly propagating sargassum seedlings by the main branch segment tillering method. Background technique [0002] Sargassum algae are dark brown, shaped like a rat tail, 3 to 50 cm high, up to 120 cm high. Grows on mid-tidal rocks or bogs. Visible throughout the year, the peak growth period is from March to July. Widely distributed in my country's coastal areas. Sage algae is rich in nutrients and low in alginate, so it is a high-quality bait for important seafood such as sea cucumbers. Sargassum was seldom used by people before, but with the rapid expansion of the scale of sea cucumber and other seafood farming, the natural growth of Sargassum has been collected in large quantities. At present, the resources of Sargassum in many breeding areas are almost exhausted. [0003] The lack of sargassum resources and the poor quality of feed have largely restricted the hea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G33/00C12N5/04
CPCY02A40/80
Inventor 叶乃好毛玉泽方建光邹健于守团
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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