Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Comprehensive detection kit for early carcinoma of prostate, detecting method and use thereof

A technology for comprehensive detection of prostate cancer, applied in the field of comprehensive detection kits in the early stage of prostate cancer, can solve problems such as unreported detection methods and applications of prostate cancer, imperfect design ideas of anti-cancer drugs, drug resistance of tumor cells, etc. Achieve high sensitivity, good sensitivity and strong specificity

Active Publication Date: 2011-09-14
台州和和生物科技有限公司
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In 2005, the American Institute of Health, the Cancer Institute, the Centers for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer" , that is to say, the cancer mortality rate has not been reduced, and it lists several factors that lead to the failure of the anti-cancer war: 1. Tumor cell heterogeneity; 2. Tumor cell drug resistance; 3. Incomplete design of anticancer drugs, etc.
However, there is no report on the comprehensive detection kit, detection method and application of prostate cancer early stage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Comprehensive detection kit for early carcinoma of prostate, detecting method and use thereof
  • Comprehensive detection kit for early carcinoma of prostate, detecting method and use thereof
  • Comprehensive detection kit for early carcinoma of prostate, detecting method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A comprehensive detection kit for early prostate cancer, including two groups of hybridization probes and markers, wherein: the hybridization probes have the sequences shown in the sequence table SEQ ID NO.1 and SEQ ID NO.2 respectively, and SEQ ID NO.2 ID NO.1 is the sequence of PSA, and SEQ ID NO.2 is the sequence of PCADM-1. Labeled with digoxin. The composition of other liquids and specimens in the kit is as follows:

[0046] Digestive solution 100μL / tube 2 tubes / box Colorless transparent liquid

[0047] Protective solution 100μL / tube 2 tubes / box Colorless transparent liquid

[0048] Pre-hybridization solution 1300μL / tube 4 tubes / box Colorless transparent liquid

[0049] Sense hybridization solution 10μL / tube 2 tubes / box Colorless transparent liquid

[0050] Antisense hybridization solution 10μL / tube 2 tubes / box Colorless transparent liquid

[0051] Blocking solution 1000μL / tube 2 tubes / box Colorless transparent liquid

[0052] Alkaline phosphatase antibody 1μ...

Embodiment 2

[0096] A kind of PCADM-1 gene and PSA gene in situ hybridization detection method and its kit application

[0097] 1. Specimen processing

[0098] 1. Use a 10ml centrifuge tube to fill 4.5ml of lymphocyte separation solution, then slowly add 3ml of anticoagulated blood into the centrifuge tube containing lymphocyte separation solution (blood: lymphocyte separation solution = 1:1.5), and centrifuge at 2000r / min 10min;

[0099] 2. Take the white blood cells in the middle layer into another centrifuge tube, then add about twice the amount of 1× buffer I to this tube, mix well, and centrifuge at 1500g / min for 10min;

[0100] 3. Discard the supernatant. Add about twice the 1× buffer I to the precipitate, mix well, and centrifuge at 1500g / min for 10min;

[0101] 4. Discard the supernatant, and absorb the excess liquid from the mouth of the test tube with paper towels. Then the precipitate was made into a suspension, dropped on a glass slide, and allowed to dry naturally. (Hospit...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a comprehensive detection reagent kit for early prostate cancer, which comprises two groups of hybridization probes and markers, wherein the hybridization probes have sequences shown in a sequence table SEQ ID NO.1 and a sequence table SEQ ID NO.2 respectively. The invention also provides an in-situ hybridization detection method for a PCADM-1 gene and a PSA gene, which comprises the following steps: a, the hybridization probes in the reagent kit contact RNA to be detected in a substrate to form a hybridization complex; and b, the hybridization complex obtained in step a is detected. The invention also provides application of the comprehensive detection reagent kit for the early prostate cancer to preparation of a medicine for comprehensively detecting an early prostate cancer disease. The reagent kit has the advantages that the reagent kit has the characteristics of high sensitivity and strong specificity. The detection method has convenient and simple operation, and can be universally used and promoted in district hospitals and above.

Description

【Technical field】 [0001] The invention relates to a reagent kit, in particular to a comprehensive detection kit, detection method and application of early prostate cancer. 【Background technique】 [0002] According to the information provided by authoritative organizations at home and abroad, there are 1.7 million new cancer cases in my country every year, nearly 1.6 million deaths, and 6 million patients. In the world, there are 8 million new cancer patients every year, nearly 8 million deaths, and about 84 million patients. , by 2020 the number of people will double, which is a set of terrible figures. [0003] In 2005, the United States Institute of Health, Cancer Institute, Center for Disease Control and other units made an annual report, "Considering that human beings have failed in the war against cancer", that is to say, the mortality rate of cancer has not been reduced. Several factors for the failure of the cancer war are: 1. Heterogeneity of tumor cells; 2. Drug res...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 裘建英张云福
Owner 台州和和生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com