Rebaudioside a composition and method for purifying rebaudioside a
A kind of technology of stevioside and composition, applied in the field of stevioside A composition and its purification
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Embodiment A1
[0078] The crude stevioside A (77.4% purity) mixture was obtained from commercial sources. Impurities (6.2% steviol glycosides, 5.6% steviol diglycoside C, 0.6% steviol diglycoside F, 1.0% other steviol glycosides, 3.0% steviol diglycoside D, 4.9% steviol diglycoside B, 0.3% steviol diglycoside) In the dry state (water content 4.7%), it was confirmed and quantified by HPLC.
[0079] Combine crude stevioside A (400 g), ethanol (95%, 1200 ml), methanol (99%, 400 ml) and water (320 ml) and heat to 50°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×200 ml, 95%), and dried in a vacuum oven at low pressure (20 mm) at 50° C. for 16 to 24 hours.
[0080] The final composition (130 g) of the substantially pure steviol diglycoside A contains 98.91% steviol diglycoside A, 0.06% steviol diglycoside, 0.03% steviol diglycoside C, 0.12% steviol diglycoside F, and 0.13% other steviol glycosides , 0....
Embodiment A2
[0082] Crude stevioside A (80.37% purity) was obtained from commercial sources. Impurities (6.22% steviol glycosides, 2.28% steviol glycosides C, 0.35% steviol glycosides A, 0.78% steviol glycosides F, 0.72% other steviol glycosides, 3.33% steviol glycosides B, 0.07% steviol glycosides) In the dry state (moisture content 3.4%), it was confirmed and quantified by HPLC.
[0083] Combine crude stevioside A (100g), ethanol (95%, 320ml), methanol (99%, 120ml) and water (50ml) and heat to 30-40°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×50 ml, 95%). The wet cake (88g) was slurried in ethanol (95%, 1320ml) for 16 hours, filtered, washed with ethanol (95%, 2×100ml), and placed in a vacuum oven at low pressure (20mm) 60 Dry at ℃ for 16 to 24 hours.
[0084] The final composition of the substantially pure steviol diglycoside A (72 g) contains 98.29% steviol diglycoside A, 0.03% steviol d...
Embodiment A3
[0086] Crude stevioside A (80.37% purity) was obtained from commercial sources. Impurities (6.22% steviol glycosides, 2.28% steviol glycosides C, 0.35% steviol glycosides A, 0.78% steviol glycosides F, 0.72% other steviol glycosides, 3.33% steviol glycosides B, 0.07% steviol glycosides) In the dry state (moisture content 3.4%), it was confirmed and quantified by HPLC.
[0087] Combine crude stevioside A (50 g), ethanol (95%, 160 ml), methanol (99%, 60 ml) and water (25 ml) and heat to about 30°C for 10 minutes. The clear solution was allowed to cool to 22°C for 16 hours. The white crystals were filtered and washed twice with ethanol (2×25 mL, 95%). The moist filter cake (40 g) was slurried in methanol (99%, 600 ml) for 16 hours, filtered, washed with methanol (99%, 2×25 ml), and placed in a vacuum oven at low pressure (20 mm) 60 Dry at ℃ for 16 to 24 hours.
[0088] The final composition (27.3 g) of the substantially pure steviol diglycoside A contains 98.22% steviol diglycosid...
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