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Preparation and use of polyvinyl pyrrolidon modified superparamagnetism nano bengala

A polyvinylpyrrolidone and superparamagnetic technology, applied in the biological and medical fields, can solve the problem of unsatisfactory transplantation efficiency and curative effect, lack of islet graft monitoring technology, inability to intervene early and prevent primary graft dysfunction and immunity. Repulsion reaction and other problems, to achieve the effect of strong magnetism, high dispersion and uniform particle size

Inactive Publication Date: 2009-07-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pancreatic islet transplantation is considered to be the most effective way to treat insulin-dependent diabetes mellitus, but due to the lack of effective and non-invasive islet transplant monitoring technology, early intervention and prevention of primary graft dysfunction and immune rejection cannot be prevented. , resulting in less than ideal transplantation efficiency and curative effect

Method used

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  • Preparation and use of polyvinyl pyrrolidon modified superparamagnetism nano bengala
  • Preparation and use of polyvinyl pyrrolidon modified superparamagnetism nano bengala
  • Preparation and use of polyvinyl pyrrolidon modified superparamagnetism nano bengala

Examples

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Effect test

Embodiment 1

[0044] Embodiment 1, a kind of preparation method of the superparamagnetic iron oxide nanoparticle (PVP-SPIO nanoparticle) of polyvinylpyrrolidone modification, carries out following steps successively:

[0045] 1), under the protection of the inert gas Ar, 0.27g FeCl 3 ·6H 2 O, 0.1gFeCl 2 4H 2 0.2.7gPVP is dissolved in the diethylene glycol (DEG) of 18ml, obtains yellow-brown solution;

[0046] 2), under the protection of the inert gas Ar, under stirring conditions, add a mixed solution composed of 0.16gNaOH and 9ml DEG to the above-mentioned yellow-brown solution, and the reaction solution immediately changes from yellow-brown to black-green; React for 2.5 hours, then slowly raise the temperature to 210°C, and reflux at 210°C for 2 hours; keep stirring during the reaction; finally obtain a black suspension;

[0047] 3) Cool the black suspension to room temperature, add 100ml of ethyl acetate and centrifuge at 5000rpm, remove the supernatant; obtain a black solid;

[004...

Embodiment 2

[0051] Embodiment 2, a kind of preparation method of the superparamagnetic iron oxide nanoparticle (PVP-SPIO nanoparticle) of polyvinylpyrrolidone modification, carries out following steps successively:

[0052] 1), under the protection of inert gas Ar, mix 0.54g FeCl under stirring condition at room temperature (10 ℃) 3 ·6H 2 O, 0.2gFeCl 2 4H 2 O, 8.1gPVP is dissolved in the diethylene glycol (DEG) of 36ml, obtains yellow-brown solution;

[0053] 2), under the protection of the inert gas Ar, under stirring conditions, add a mixed solution composed of 0.32g NaOH and 18ml DEG to the above yellow-brown solution, the reaction solution immediately changes from yellow-brown to black-green; React for 3 hours, then slowly raise the temperature to 220°C, and reflux at 220°C for 2 hours; keep stirring during the reaction; finally obtain a black suspension;

[0054] 3) Cool the black suspension to room temperature, add 200ml of ethyl acetate and centrifuge, remove the supernatant; o...

Embodiment 3

[0058] Embodiment 3, a kind of preparation method of the superparamagnetic iron oxide nanoparticle (PVP-SPIO nanoparticle) of polyvinylpyrrolidone modification, carries out following steps successively:

[0059] 1), under the protection of the inert gas Ar, 0.27g FeCl 3 ·6H 2 O, 0.1gFeCl 2 4H 2 0.3.4gPVP is dissolved in the diethylene glycol (DEG) of 18ml, obtains yellow-brown solution;

[0060]2), under the protection of the inert gas Ar, under stirring conditions, add a mixed solution composed of 0.16gNaOH and 9ml DEG to the above-mentioned yellow-brown solution, and the reaction solution immediately changes from yellow-brown to black-green; React for 2.5 hours, then slowly raise the temperature to 215°C, and reflux at 215°C for 1.5 hours; keep stirring during the reaction; finally obtain a black suspension;

[0061] 3) Cool the black suspension to room temperature, add 100ml of ethyl acetate and centrifuge, remove the supernatant; obtain a black solid;

[0062] 4), dis...

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Abstract

The invention discloses a method for preparing superparamagnetic iron oxide nanometer particles modified by polyvinylpyrrolidone. The method sequentially comprises the following steps that: 1) under the protection of inert gas, FeCl3.6H2O, FeCl2.4H2O and polyvinylpyrrolidone are dissolved in diglycol at the room temperature; 2) under the protection of inert gas, mixed solution of NaOH and diglycol is added to obtained tawny solution for reaction; 3) an obtained black suspension is cooled to room temperature and added with ethyl acetate for centrifugation; 4) solids obtained after centrifugation are dispersed in anhydrous ethanol, and then ethyl acetate is added for centrifugation; 5) the steps 4) is repeated twice to three times and black solids are obtained; and 6) the black solids are dispersed in deionized water, placed in a dialysis bag with the molecular weight cutoff between 10 and 15 w and dialyzed for at least 48 hours. The superparamagnetic iron oxide nanometer particles modified by polyvinylpyrrolidone are used for tracing the functions of islet graft.

Description

technical field [0001] The invention belongs to the technical field of biology and medicine, and relates to material chemistry, in particular to synthesizing polyvinylpyrrolidone-modified superparamagnetic iron oxide (PolyvinylpyrrolidoneCoated Superparamagnetic Iron Oxide, PVP-SPIO) with good biocompatibility through high-temperature polyol process technology Nanoparticles, the present invention also relates to the use of the nanoparticles in medicine. Background technique [0002] With the improvement of living standards, diabetes has become a major social problem that threatens the health and life of all human beings, and has caused a huge economic burden to the society. Pancreatic islet transplantation is considered to be the most effective way to treat insulin-dependent diabetes mellitus. However, due to the lack of effective and non-invasive islet graft monitoring technology, early intervention and prevention of primary graft dysfunction and immune rejection cannot be ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G49/08A61K49/00
Inventor 吴育连黄海
Owner ZHEJIANG UNIV
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