Method for propagating Tortula muralis protonema by cell engineering cultivation

Abstract

The invention relates to a method for cultivating and breeding pangenesis tortula protonema by using cell engineering, which belongs to the technical field of biology and comprises following concrete steps: a. mature spores are sterilized, and capsulea surface is sterilized for 0.5 to 1 minutes by a solution that contains 1.0% of mercury bichloride and 1.0% of a washing agent; b. germination is implemented on a solid culture medium, the spores are inoculated to an MS culture medium that has the pH value of 5.8 and is added with 2 to 5 mg / L of 2,4-D, 2.0g / L of sodium silicate and 2.0% of sucrose, and cultivated at the temperature of 25 plus or minus 3 DEG C and illumination intensity of 4000lx for 10 to 20 days; c. mass propagation of the protonema is implemented in the MS fluid culture medium for spore germination that has pH value of 5.8 and is added with 2 to 5 mg / L of 2,4-D, 2.0g / L of sodium silicate and 2.0% of sucrose, shaking cultivation is implemented at the temperature of 25 plus or minus 3 DEG C and the speed of 120rpm for 7 to 10 days and the power source is diffused lights; or augmentation cultivation is implemented in a fermentation flask charged with sterile air at the speed of 200L per hour; a small amount of the protonema is collected by 800rpm centrifugation, and a large amount of the protonema is collected through the filtration by 4 to 8 gauze layers. The invention has the advantages of simple and convenient preparation method, low cost and consistent maturity of the obtained protonema.

Description

Technical field:

[0001] The invention relates to a method for cultivating and propagating protocelium of Phyllostachys panphyta by cell engineering, which belongs to the field of biotechnology. Background technique:

[0002] In the plant kingdom, bryophytes are a group of plants that transition from aquatic life to terrestrial life, and are one of the oldest groups of terrestrial plants on earth. At present, there are footprints of bryophytes in different ecosystems on the earth, including Antarctica, except for mountains and oceans above 5,000 meters. Bryophytes belong to the spore plants in higher plants. There are about 23,000 species in the world, and the number of species is second only to angiosperms. It is an important category in the plant kingdom.

[0003] The life history of bryophytes is dominated by gametophytes, and the common green bryophytes belong to the gametophyte generation. The sporophyte of bryophytes is attached to the gametophyte, sometimes the sporo...