Method for separating cytospaz total alkaloid from traditional Chinese medicine extract liquid

Abstract

The invention discloses a method for separating total alkaloid from traditional Chinese medicine, in particular a method for separating belladonna total alkaloid from traditional Chinese medicine extract, which belongs to the field of traditional Chinese medicines. The method comprises the following technical proposal that the traditional Chinese medicine extract is taken and filtered; the pH value of the traditional Chinese medicine extract is adjusted to be between 1 and 7; filtrate is added to a cation exchange resin column, eluted with water to remove impurities and then eluted by use of 0.5 to 15 percent acid liquid; and eluent is collected, added with alkali for neutralization and desalted so as to prepare belladonna total alkaloid. The method overcomes the deficiency that the prior art is low in extraction ratio, large in the amount of organic solvents, complex in process, incapable of mass production, and the like, and can efficiently separate high-purity belladonna total alkaloid from traditional Chinese medicine extract.

Description

technical field [0001] The invention relates to a method for extracting and separating active ingredients of traditional Chinese medicines, in particular to a method for extracting and separating total alkaloids of hyoscyamine from traditional Chinese medicine extracts, which belongs to the field of traditional Chinese medicines. Background technique [0002] Traditional Chinese medicine alkaloids are an important class of substances existing in nature, and more than 700 species have been isolated so far. They are the largest class of active ingredients in traditional Chinese medicine with definite curative effects and a wide range of uses, especially for cancer, liver disease, cardiovascular and cerebrovascular diseases, and rheumatism. etc. have unique curative effects, such as ephedrine in ephedra for relieving asthma, reserpine in Rauwolfia for lowering blood pressure, galantamine in lycoris for sequelae of poliomyelitis, and the Morphine is a famous analgesic; quinine i...

AI Technical Summary

Problems solved by technology

But for the refinement of alkaloids, the biggest deficiency of this method is: firstly, it is difficult to adsorb, and the sample medicine solution must be a dilute solution, and the alkalinity should be suitable. The rate is low, the alkalinity is high, the alkaloids are free, and the macromolecular alkaloids are poorly soluble and precipitated, and they still cannot be loaded on the column. The range of applicable alkaloids is very small and there are too few varieties; The separation selectivity of the adsorption principle to the active ingredients of alkaloids and fat-soluble impurities is too poor, and in the process of solvent elution, because there is no specific elution solvent, alkaloids and a large amount of fat-soluble impurities are often washed down together, and finally refined to obtain The purity of the total alkaloids is not high

However, the steps of this method are cumbersome, and steps such as organic solvent extraction and resin column reflux extraction with organic solvent are difficult to realize in industry, and the efficiency of elution with lye is very low, and it only stays at the level of preparing small samples in the laboratory. Unable to achieve mass production

[0007] The above methods generally have the disadvantages of high cost, large consumption of organic solvents, poor selectivity, low purity of total alkaloids, and inability to realize large-scale production. Therefore, purification technology is still a "bottleneck" problem for the separation of alkaloids in traditional Chinese medicine.

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