Clone and expression of 7,8-desaturase gene

Abstract

The invention provides clone and expression aiming at 7,8-dehydrogenase gene. The gene encoding 7,8-dehydrogenase is acquired from a constructed drosophila cDNA library, wherein the 7,8-dehydrogenase can transform cholesterol into 7-dehydrocholesterol, thereby providing a new method for synthesizing the 7-dehydrocholesterol by a biological process, acquiring a process route capable of shortening vitamin D3, reducing production cost, and reducing environmental pollution.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and enzyme engineering, and specifically relates to the cloning and expression of 7,8-dehydrogenase genes. Background technique [0002] Vitamin D 3 It is an essential fat-soluble vitamin for the growth, development, reproduction, maintenance of life and health of humans and animals. Its main function is to regulate calcium and phosphorus metabolism, promote intestinal calcium and phosphorus absorption and bone calcification, maintain the balance of blood calcium and blood phosphorus, and can be used as pharmaceutical preparations, food and feed additives, etc., and can also be used clinically to treat rickets , senile osteoporosis, hypothyroidism, etc. current vitamin D 3 It is produced by photochemical method, that is, 7-dehydrocholesterol (7-DHC) is used as raw material, and it is converted into vitamin D after ultraviolet irradiation. 3 Precursor (PreD 3 ), obtained by reheating isomer...

AI Technical Summary

Problems solved by technology

But so far, no matter what kind of chemical synthesis method is used to prepare raw material 7-dehydrocholesterol, there are disadvantages such as long reaction steps, lo

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