42 results about "7-Dehydrocholesterol" patented technology

The present invention relates to a 25-hydroxy-7-dehydrocholesterol synthetic method, a 25-hydroxy-7-keto-cholesterol derivative is used as a raw material for mechanical milling reaction in a ball mill pot under the effect of sulfonyl hydrazide and a base in the absence of an organic solvent, and then hydrolysis reaction is performed to obtain 25-hydroxy-7-dehydrocholesterol synthetic method. According to the method, in the absence of a solvent, mechanical milling reaction is performed, no dust is spilled over in the grinding process, reaction controllability is good, reaction efficiency is high, the solvent recovery operation in the post-processing process can be avoided, operation is simplified, environmental pollution is reduced, the reaction selectivity is good, yield is high, operation is simple, cost is low, post-treatment is simple, three-waste is less, product is easy to separate, purity is high, the yield is up to 89%, the melting process is short, and the product purity is high.

The invention relates to a method for preparing 7-dehydrocholesterol, which comprises the following steps: 1) protecting hydroxyl on the 3-site of cholesterol; 2) oxidizing carbon on the 7-site of the product obtained in step 1) into carbonyl; 3) carrying out a hydrazone reaction on the product obtained in step 2) and a hydrazine derivative; 4) reacting the product obtained in step 3) with a strongly alkaline reagent; and 5) reacting the product obtained in step 4) with water or an acid. The method has the advantages of simple operation process, less secondary reaction, high yield and less corrosion to equipment, thereby having high industrial production values.

The invention discloses a saccharomyces cerevisiae strain for producing 7-dehydrocholesterol and a construction method. The construction method comprises the steps as follows: 1), construction of carriers: a carrier SyBE_000923 is constructed and a carrier SyBE_000937 is constructed; 2), a saccharomyces cerevisiae strain SyBE_000954 is obtained; and 3), the carrier SyBE_000923 and the carrier SyBE_000937 are guided into the saccharomyces cerevisiae strain SyBE_000954, so that the saccharomyces cerevisiae strain for producing 7-dehydrocholesterol is obtained. According to the saccharomyces cerevisiae strain and the construction method, a synthetic biotechnology is utilized, so that the artificially synthesis of the 7-dehydrocholesterol is realized, not only can the defects of a chemical method be made up, but also the production process is environment-friendly and clean.

The invention discloses a preparation method of 25-hydroxyl-7-dehydrocholesterol. The preparation method takes 5,7,24-trienecholesterol as a raw material; 3-site hydroxyl of the raw material is protected to prepare an intermediate compound II; the intermediate compound is subjected to D-A protection reaction of diene to obtain an intermediate compound III; the intermediate compound III is subjected to double-bond halogenation and hydroxylation reaction to obtain an intermediate compound IV; the intermediate compound IV is subjected to de-protection and debromination reaction to prepare the 25-hydroxyl-7-dehydrocholesterol. By adopting the method provided by the invention, the 25-hydroxyl-7-dehydrocholesterol is obtained; compared with a preparation method reported in existing literature reports, the product yield is high and the purity is good; the reaction selectivity is relatively good and reaction operation is simple; pollution caused by a mercury-containing toxic and harmful reagent is effectively avoided; the cost of three wastes is reduced and the preparation method is suitable for industrialized popularization and application.

The invention relates to a method for photochemically synthesizing vitamin D2 and D3 in a tubular reactor. The method is characterized in that the vitamin D2 and D3 are synthesized by ultraviolet light of different bands, the residence time of reaction liquid fed at different times in the tubular reactor is controlled to be the same by not dissolving with reaction liquid, not participating in reaction liquid separation and carrying ergosterol and 7-dehydrocholesterol reaction liquid and enhancing internal disturbance of the reaction liquid; the method has the advantages of continuous, stable and high efficiency of solar energy utilization, the operation is simple, and the process is controllable; the method has great prospect in the industrial production of the photochemically synthesizedvitamin D2 and D3.

The invention discloses a preparation method of 7-dehydrocholesterol. The preparation method comprises four steps of esterifying, brominating, de-brominating, saponifying and hydrolyzing. In the brominating reaction process, the brominating agent, namely N-brominated succimide (NBS) with advantages of high location, high active bromine content, good storage stability and economic usage is selected. Due to the brominating agent, the side reaction is less during the preparation; the by-product is few and a plurality of steps of removing brominating by-products are saved; due to the mol concentration proportion of the method, the yield of the target product is notably improved.

A novel synthesis technology of 7-dehydrocholesterol employs an esterification reaction, a high positioning brominated reaction, a de-bromination reaction and saponification hydrolysis. The high positioning brominated reaction of the technology employs a high positioning brominated agent N-brominated succimide (NBS), which has advantages of high positioning, high active bromine content, good storage stability and thrift for usage. The brominated agent can reduce side reaction and by-product and omit many steps for removing brominated by-products. Besides, the technology employs a benzoyl chloride esterification method, has simple production technology and small equipment investment and is easy for operation. According to the innovative technology, a yield reaches 75%, which is 50% higher than a yield of 25% of other manufacturer; and a product content reaches 98%, which is 3% higher than a product content of 95% of an prior art.

The invention belongs to the technical field of biochemical engineering and enzyme engineering, and in particular relates to a method utilizing the microsomal enzyme of an animal liver to biosynthesize 7-dehydrocholesterol. The method includes the following steps: (1) preparation of microsomal enzyme solution; (2) enzymatic conversion reaction; (3) saponification reaction; and (4) extraction. The method can prepare the 7-dehydrocholesterol which is completely identical with that prepared by chemosynthesis; and meanwhile, the technology is simple and easy, and not only the production cost of vitamin D3 can be reduced, but also the environment pollution can be reduced.

The invention discloses a liquid 25-hydroxyvitamin D calibrator diluent, which comprises the following components: a buffer solution, a protein stabilizer, a preservative and a cyclopentane polyhydrophenanthrene stabilizer. The main innovation of the invention lies in that a) 7-dehydrocholesterol is applied to the 25-hydroxyvitamin D calibrator for the first time for improving the stability of 25-hydroxyvitamin D under a liquid aerobic condition; b) all the components in the calibrator diluent are optimal formulas, so that the stability of the 25-hydroxyvitamin D calibrator is powerfully guaranteed, and the 25-hydroxyvitamin D calibrator can be stably stored at 8 DEG C for 12 months; and c) the liquid 25-hydroxyvitamin D calibrator is applied to detection of 25-hydroxy vitamin D in the serum sample, the calibrator does not need to be redissolved during detection, and the repeatability of a calibration curve which is calibrated for multiple times is superior to that of the calibration curve of the freeze-dried calibrator.

The invention belongs to the technical field of genetic engineering and discloses application of an acetyl coenzyme A enhancing module to enhancement of 7-dehydrocholesterol synthesis in microorganisms. According to the application, the acetyl coenzyme A enhancing module comprises genes expressing ethanol dehydrogenase, genes expressing acetaldehyde dehydrogenase, genes expressing acetyl coenzyme A synthase and genes expressing ATP-citrate lyase. After the acetyl coenzyme A enhancing module is led in the microoranisms, the synthesis amount of the 7-dehydrocholesterol is remarkably increased. A new method is provided for synthesizing 7-dehydrocholesterol, and mass production of 7-dehydrocholesterol is facilitated better.

The invention provides clone and expression aiming at 7,8-dehydrogenase gene. The gene encoding 7,8-dehydrogenase is acquired from a constructed drosophila cDNA library, wherein the 7,8-dehydrogenase can transform cholesterol into 7-dehydrocholesterol, thereby providing a new method for synthesizing the 7-dehydrocholesterol by a biological process, acquiring a process route capable of shortening vitamin D3, reducing production cost, and reducing environmental pollution.

The invention discloses a production method of 7-dehydrocholesterol. The method includes the steps: S1 dissolving cholesterol and benzoyl chloride in a solvent, and performing acylation reaction to obtain cholesteryl benzoate; S2 dissolving the cholesteryl benzoate and 1, 3-dibromo-5, 5-dimethyl hydantoin in the solvent, adding initiating agents, performing hydrazone reaction to obtain 7-bromo cholesterol benzoate; S3 dissolving the 7-bromo cholesterol benzoate and pyridine in ethyl alcohol, and performing hydrazone removal reaction to obtain 7-dehydrocholesterol benzoate; S4 adding the 7-dehydrocholesterol benzoate into sodium hydroxide solution, and performing saponification hydrolysis reaction to obtain the 7-dehydrocholesterol. The production method for the 7-dehydrocholesterol has the advantages of reasonable process, mild reaction condition, high selectivity, simplicity in operation, friendly environment and low production cost.

The invention discloses a method for purifying the intermediate 7-dehydrocholesterol leftovers of vitamin D3, which aims to solve the problem that the 7-dehydrocholesterol leftovers cannot be used and are discarded in industrial production due to the existence of a large amount of 3-hydroxycholestane-4,6-diene and the leftovers thereof to cause the high production cost of the vitamin D3 and environmental pollutions. The method comprises the following steps of: dissolving the 7-dehydrocholesterol leftovers containing the byproduct 3-hydroxycholestane-4,6-diene into an alcohol solvent in a volume which is twice to 10 times the weight of the leftovers, and stirring the mixture until the leftovers are completely dissolved; and adding an iron salt or aluminum salt into the system, continuing to perform stirring until the impurity 3-hydroxycholestane-4,6-diene is completely reacted, performing extraction on the obtained solution by using varsol, and then separating and removing the 3-hydroxycholestane-4,6-diene by using a chromatographic column. In the method, ether impurities produced in the reaction process can be easily separated from 7-dehydrocholesterol, so the treatment process is greatly reduced.

The invention discloses a chicken feed capable of improving egg yield. The chicken feed is prepared by the following steps: mixing 43-50 parts of corn, 22-26 parts of bean cake, 10-11 parts of wheat bran, 1 part of fish meal, 0.2 part of salt, 10 parts of oyster shell, 3 parts of sodium alga acid, 0.7 part of 7-dehydrocholesterol, 0.1 part of cholestanol, 0.4 part of sodium hexametaphosphate, 2.5 parts of an organic iron-zinc enzymic preparation, 0.1 part of pentaerythritol ester of rosin, 0.1 part of sodium potassium tartrate tetrahydrate and 0.1-0.3 part of sodium glutamate evenly; crushing, and drying to obtain the chicken feed. According to the obtained chicken feed, absorption of chicken and improvement of the egg yield of the chicken are facilitated; chicken can be prevented from being ill; and the chicken feed has the characteristics of wide raw material source and comprehensive nutrients.

The invention discloses a method for preparing vitamin D3 and used equipment. In the prior method, a solvent must be replaced during the crystallization process of the post-treatment, and the heating time is long, which affect the content of a final product. A solvent used by the method in a photochemical reaction is a mixed solvent consisting of a high melting point solvent and a low melting point solvent; an obtained photochemical reaction liquid is subjected to freezing crystallization and filtering post-treatment after the photochemical reaction is finished; and crude vitamin D3 oil is obtained after filtrate is concentrated; after a filter cake is heated and dissolved, 7-dehydrocholesterol and the low melting point solvent which is concentrated from the filtrate are added to serve as raw materials for the photochemical reaction; the high melting point solvent is alcoholic solvents or hydrocarbon solvents with melting points of between 10 DEG C below zero and 10 DEG C, and the low melting point solvent is alcohol solvents with melting points less than or equal to 50 DEG C below zero. The vitamin D3 obtained by the method has high content and less polymerization side reactions, does not need to replace the solvent during the post-treatment process, and has good quality.

In one embodiment, the present application discloses an aqueous ophthalmic composition for the treatment of eye diseases, lesions and injuries, comprising: a) one steroid, or a combination of at least two steroids selected from the group consisting of lanosterol, dihydrolanosterol, 4,4-dimethylcholesta-8(9),14,24-trien-3β-ol, 4,4-dimethylcholesta-8,24-dien-3β-ol, 4,4-dimethylcholesta-8-en-3β-ol, 4,4-dimethylcholesta-8(9),14-dien-3β-ol, 14-desmethyl lanosterol, lathosterol, Δ^7,24-cholestadienol, cholesterol, cholesta-7-enol, cholesteryl ester, 7-dehydrocholesterol, desmosterol, 7-dehydrodesmosterol, zymosterol, 27-hydroxycholesterol, cholesta-7,24-dien-3-β-ol, cholesta-8(9)-en-3-β-ol, 5α-cholestan-3β-ol-6-one, 5-cholesten-3β,25-diol, 5-cholesten-3β,25-OSO₃H (5-cholesten-3β,25-sulfate), 5-cholesten-3β-OSO₃H,25-ol (5-cholesten-3β-sulfate,25-ol), 5-cholesten-3β,25-diol, disulfate, and their esters thereof, or a pharmaceutically acceptable salt thereof, in a concentration effective for the treatment and/or prophylaxis of the eye diseases, lesions and injuries; and b) a pharmaceutical excipient; and methods of treatment using such compositions.

The invention belongs to the field of gene engineering, and particularly relates to a prawn Y-organ 7,8-dehydrogenase gene and application thereof. The invention provides a gene for coding 7,8-dehydrogenase, which is obtained from a constructed Metapenaeus ensis Y-organ cDNA (complementary deoxyribonucleic acid) library; and the nucleotide sequence of the gene is disclosed as SEQ ID NO.1. The application method comprises the following steps: cloning the 7,8-dehydrogenase coding gene, and connecting with an expression plasmid to construct a recombinant expression plasmid; after linearizing and purifying the recombinant expression plasmid, converting a receptor bacterium, and screening to obtain a gene engineering bacterium; and carrying out induction expression, purification and identification to obtain the 7,8-dehydrogenase of which the amino acid sequence is disclosed as SEQ ID NO.2. The 7,8-dehydrogenase can be used for converting cholesterol into 7-dehydrocholesterol by a one-step enzyme process, thereby enhancing the conversion efficiency, shortening the process route for producing vitamin D3, lowering the production cost and reducing the environmental pollution.