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42 results about "7-Dehydrocholesterol" patented technology
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7-Dehydrocholesterol (7-DHC) is a zoosterol that functions in the serum as a cholesterol precursor, and is photochemically converted to vitamin D₃ in the skin, therefore functioning as provitamin-D₃. The presence of this compound in human skin enables humans to manufacture vitamin D₃ (cholecalciferol) from ultraviolet rays in the sun light, via an intermediate isomer pre-vitamin D₃. It is also found in the milk of several mammalian species. In insects it is a precursor for the hormone ecdysone, required for reaching adulthood. It was discovered by Nobel-laureate organic chemist Adolf Windaus.
Preparation method for 7-dehydrochol esterol
The invention discloses a preparation method of a 7-dehydrocholesterol, which comprises the following steps: a) protecting a hydroxyl on the 3 position of a cholesterol; b) oxidizing a carbon on the 7 position of the product made in step a) into a carbonyl; c) carrying out hydrazone reaction to the product made in step b) with hydrazine derivatives, the structure of the hydrazine derivatives being at most one H to be substituted by the following hydrazine; d) reacting the product made in step c) with a strong basic reagent having stronger alkaline than a sodium hydroxide; e) reacting the product made in step d) with water or an acid. In the preparation method, no halogen group elements such as Br element are participated, the side effects are less and the yield is high, and the prepared product is safe and innocuous.
Owner:BEIJING UNIV OF CHEM TECH
25-hydroxy-7-dehydrocholesterol synthetic method
The present invention relates to a 25-hydroxy-7-dehydrocholesterol synthetic method, a 25-hydroxy-7-keto-cholesterol derivative is used as a raw material for mechanical milling reaction in a ball mill pot under the effect of sulfonyl hydrazide and a base in the absence of an organic solvent, and then hydrolysis reaction is performed to obtain 25-hydroxy-7-dehydrocholesterol synthetic method. According to the method, in the absence of a solvent, mechanical milling reaction is performed, no dust is spilled over in the grinding process, reaction controllability is good, reaction efficiency is high, the solvent recovery operation in the post-processing process can be avoided, operation is simplified, environmental pollution is reduced, the reaction selectivity is good, yield is high, operation is simple, cost is low, post-treatment is simple, three-waste is less, product is easy to separate, purity is high, the yield is up to 89%, the melting process is short, and the product purity is high.
Owner:ZHEJIANG UNIV OF TECH +2
Method for preparing 7-dehydrocholesterol
The invention relates to a method for preparing 7-dehydrocholesterol, which comprises the following steps: 1) protecting hydroxyl on the 3-site of cholesterol; 2) oxidizing carbon on the 7-site of the product obtained in step 1) into carbonyl; 3) carrying out a hydrazone reaction on the product obtained in step 2) and a hydrazine derivative; 4) reacting the product obtained in step 3) with a strongly alkaline reagent; and 5) reacting the product obtained in step 4) with water or an acid. The method has the advantages of simple operation process, less secondary reaction, high yield and less corrosion to equipment, thereby having high industrial production values.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES
Saccharomyces cerevisiae strain for producing 7-dehydrocholesterol and construction method
InactiveCN103275997ARealize artificial synthesisMake up for deficienciesFungiMicroorganism based processesBiotechnologyEcology
The invention discloses a saccharomyces cerevisiae strain for producing 7-dehydrocholesterol and a construction method. The construction method comprises the steps as follows: 1), construction of carriers: a carrier SyBE_000923 is constructed and a carrier SyBE_000937 is constructed; 2), a saccharomyces cerevisiae strain SyBE_000954 is obtained; and 3), the carrier SyBE_000923 and the carrier SyBE_000937 are guided into the saccharomyces cerevisiae strain SyBE_000954, so that the saccharomyces cerevisiae strain for producing 7-dehydrocholesterol is obtained. According to the saccharomyces cerevisiae strain and the construction method, a synthetic biotechnology is utilized, so that the artificially synthesis of the 7-dehydrocholesterol is realized, not only can the defects of a chemical method be made up, but also the production process is environment-friendly and clean.
Owner:TIANJIN UNIV
Method for synthesizing resinous vitamin D3 by using micro-flow photoreaction technology and micro-flow photochemical reactor
ActiveCN103553993AEasy to makeEasy to operateOrganic chemistryAnimal feeding stuffPeristaltic pumpIsomerization
The invention belongs to the field of organic photochemistry synthesis, and in particular relates to a method for synthesizing a resinous vitamin D3 by using a micro-flow photoreaction technology, and a micro-flow photochemical reactor for synthesizing the resinous vitamin D3. The method for synthesizing the resinous vitamin D3 by using the micro-flow photoreaction technology comprises the steps of performing illumination reaction in the micro-flow photochemical reactor under the protection of nitrogen (preferably high-purity nitrogen); controlling the temperature of a photochemical reaction solution during reaction to be lower than 28 DEG C, and adjusting and controlling the flow rate of the photochemical reaction solution in a micro-flow pipe by using a peristaltic pump, wherein the conversion rate of 7-dehydrocholesterol after the illumination reaction reaches 90.5-95%, and the HPLC (high-performance liquid chromatography) yield of a pre-vitamin D3 is improved to 70-73.1%. The resinous vitamin D3 is prepared through thermal isomerization reaction, and the HPLC yield of the resinous vitamin D3 is 68-71.1%. The whole preparation process is simple and easy to operate, so that large-scale production can be realized easily.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Implant modified with non-hydroxylated vitamin d precursors
InactiveUS20130017234A1Promote osseointegrationLow toxicityBiocideImpression capsAntioxidantHydroxylation
An implant to be used as medical or dental implant, comprising a metallic or polymeric base which is covered by the vitamin D precursor cholecalciferol. The implant can be obtained by direct covering of the polymeric or metallic base with a solution comprising cholecalciferol or also covering the base with the 7-dehydrocholesterol (7-DHC), and subsequently irradiated with UV light to induce the formation of cholecalciferol. Optionally, the coating of the implant may include an antioxidant such as vitamin E. This implant enhances osseointegration in compromised patients by means of the endogenous synthesis and activity of vitamin D in hard and mineralized tissue regeneration. Furthermore, a method to obtain these implants which comprises coating the surface of the implant directly with cholecalciferol or with a specific concentration of 7-DHC and irradiated with UV light to induce the formation of cholecalciferol.
Owner:NUMAT BIOMEDICAL
Preparation method of 25-hydroxyl-7-dehydrocholesterol
The invention discloses a preparation method of 25-hydroxyl-7-dehydrocholesterol. The preparation method takes 5,7,24-trienecholesterol as a raw material; 3-site hydroxyl of the raw material is protected to prepare an intermediate compound II; the intermediate compound is subjected to D-A protection reaction of diene to obtain an intermediate compound III; the intermediate compound III is subjected to double-bond halogenation and hydroxylation reaction to obtain an intermediate compound IV; the intermediate compound IV is subjected to de-protection and debromination reaction to prepare the 25-hydroxyl-7-dehydrocholesterol. By adopting the method provided by the invention, the 25-hydroxyl-7-dehydrocholesterol is obtained; compared with a preparation method reported in existing literature reports, the product yield is high and the purity is good; the reaction selectivity is relatively good and reaction operation is simple; pollution caused by a mercury-containing toxic and harmful reagent is effectively avoided; the cost of three wastes is reduced and the preparation method is suitable for industrialized popularization and application.
Owner:ZHEJIANG UNIV OF TECH
Method for photochemically synthesizing vitamin D2 and D3 in tubular reactor
The invention relates to a method for photochemically synthesizing vitamin D2 and D3 in a tubular reactor. The method is characterized in that the vitamin D2 and D3 are synthesized by ultraviolet light of different bands, the residence time of reaction liquid fed at different times in the tubular reactor is controlled to be the same by not dissolving with reaction liquid, not participating in reaction liquid separation and carrying ergosterol and 7-dehydrocholesterol reaction liquid and enhancing internal disturbance of the reaction liquid; the method has the advantages of continuous, stable and high efficiency of solar energy utilization, the operation is simple, and the process is controllable; the method has great prospect in the industrial production of the photochemically synthesizedvitamin D2 and D3.
Owner:SHANDONG TSINGCHUANG CHEMICALSCO LTD +1
Photochemical process for the production of previtamin D3
A photochemical process for the production of previtamin D3 or a derivative thereof from 7-dehydrocholesterol is disclosed. The process includes irradiating the 7-dehydrocholesterol in a reactor with a UV radiation source, wherein the UV source includes an excimer or exciplex emitter which emits quasi-monochromatically according to the corona discharge mechanism in the UV range, and recovering previtamin D3 or a derivative thereof. Previtamin D3 compositions and derivatives made from this process are also provided.
Owner:DSM NUTRITIONAL PROD
Preparation method of 7-dehydrocholesterol
InactiveCN102702295ARapid positioningHigh active bromine contentSteroidsChemical recyclingBromineSide reaction
The invention discloses a preparation method of 7-dehydrocholesterol. The preparation method comprises four steps of esterifying, brominating, de-brominating, saponifying and hydrolyzing. In the brominating reaction process, the brominating agent, namely N-brominated succimide (NBS) with advantages of high location, high active bromine content, good storage stability and economic usage is selected. Due to the brominating agent, the side reaction is less during the preparation; the by-product is few and a plurality of steps of removing brominating by-products are saved; due to the mol concentration proportion of the method, the yield of the target product is notably improved.
Owner:HENAN LIWEI BIOLOGICAL PHARMA
Novel synthesis technology of 7-dehydrocholesterol
A novel synthesis technology of 7-dehydrocholesterol employs an esterification reaction, a high positioning brominated reaction, a de-bromination reaction and saponification hydrolysis. The high positioning brominated reaction of the technology employs a high positioning brominated agent N-brominated succimide (NBS), which has advantages of high positioning, high active bromine content, good storage stability and thrift for usage. The brominated agent can reduce side reaction and by-product and omit many steps for removing brominated by-products. Besides, the technology employs a benzoyl chloride esterification method, has simple production technology and small equipment investment and is easy for operation. According to the innovative technology, a yield reaches 75%, which is 50% higher than a yield of 25% of other manufacturer; and a product content reaches 98%, which is 3% higher than a product content of 95% of an prior art.
Owner:薛家禄
Method utilizing microsomal enzyme of animal liver to biosynthesize 7-dehydrocholesterol
The invention belongs to the technical field of biochemical engineering and enzyme engineering, and in particular relates to a method utilizing the microsomal enzyme of an animal liver to biosynthesize 7-dehydrocholesterol. The method includes the following steps: (1) preparation of microsomal enzyme solution; (2) enzymatic conversion reaction; (3) saponification reaction; and (4) extraction. The method can prepare the 7-dehydrocholesterol which is completely identical with that prepared by chemosynthesis; and meanwhile, the technology is simple and easy, and not only the production cost of vitamin D3 can be reduced, but also the environment pollution can be reduced.
Owner:GUANGXI UNIV
Method for preparing 7-dehydrogenation cholesterol
The present invention discloses a preparation method of 7-dehydrocholesterol. The saponified waste from the prior production of vitamin D3 is always treated as waste liquid, which wastes the resources greatly and causes the environmental pollution. The 3-carbonyl holest-4, 6-diene is prepared by the oxidation reaction of the 3-hydroxyl cholest-4, 6-diene vitamin D3 and weak oxidants under the nonpolar or weak polar solvent; then the mixture of 3-carbonyl holest-4, 6-diene and 3-hydroxyl cholest-5, 7-diene is prepared by the reduction reaction of the 3-carbonyl holest-4, 6-diene under weak polar solvent; the crystal of 3-hydroxyl cholest-5, 7-diene and the filtrate rich in 3-hydroxyl cholest-4, 6-diene is obtained after recrystallization purification of the mixture, and the filtrate can be used as materials of oxidation reaction after eliminating the solvent. The present invention prepares the 7-dehydrocholesterol with saponified waste, thereby the saponified waste is used efficiently and the environmental pollution is reduced.
Owner:ZHEJIANG NHU CO LTD
Enzymatic method to produce 7-dehydropregnenolone, vitamin D3-like compounds and derivatives thereof
Owner:UNIV OF TENNESSEE RES FOUND
Method for preparing acetyl cholesterol-7-one p-toluenesulfonylhydrazone
ActiveCN110143993AIncrease contentHigh product yieldSteroidsChemical recyclingOrganic synthesisContact reactions
The invention relates to the field of organic synthesis, and discloses a method for preparing acetyl cholesterol-7-one p-toluenesulfonylhydrazone. The method comprises the following steps: carrying out a contact reaction on acetyl cholesterol-7-one and p-methylbenzenesulfonhydrazide under a condensation reaction condition in a solvent containing cation exchange resin. According to the method for preparing the acetyl cholesterol-7-one p-toluenesulfonylhydrazone, acetyl cholesterol-7-one p-toluenesulfonylhydrazone with a relatively high content can be obtained under mild reaction conditions, sothat the yield of subsequent preparation of 7-dehydrocholesterol is improved. Meanwhile, a catalyst in the method is easy to recycle after the reaction, so that overall cost is reduced.
Owner:江西天新药业股份有限公司
Liquid 25-hydroxyvitamin D calibrator diluent
InactiveCN109632444AEasy to produceEasy to operatePreparing sample for investigationBiological testingFreeze-dryingDehydrocholesterols
The invention discloses a liquid 25-hydroxyvitamin D calibrator diluent, which comprises the following components: a buffer solution, a protein stabilizer, a preservative and a cyclopentane polyhydrophenanthrene stabilizer. The main innovation of the invention lies in that a) 7-dehydrocholesterol is applied to the 25-hydroxyvitamin D calibrator for the first time for improving the stability of 25-hydroxyvitamin D under a liquid aerobic condition; b) all the components in the calibrator diluent are optimal formulas, so that the stability of the 25-hydroxyvitamin D calibrator is powerfully guaranteed, and the 25-hydroxyvitamin D calibrator can be stably stored at 8 DEG C for 12 months; and c) the liquid 25-hydroxyvitamin D calibrator is applied to detection of 25-hydroxy vitamin D in the serum sample, the calibrator does not need to be redissolved during detection, and the repeatability of a calibration curve which is calibrated for multiple times is superior to that of the calibration curve of the freeze-dried calibrator.
Owner:AUTOBIO DIAGNOSTICS CO LTD
Application of acetyl coenzyme A enhancing module to enhancement of 7-dehydrocholesterol synthesis in microorganisms
InactiveCN104988168AEase of mass productionIncrease supplyFungiFermentationEthanol dehydrogenaseGenetic engineering
The invention belongs to the technical field of genetic engineering and discloses application of an acetyl coenzyme A enhancing module to enhancement of 7-dehydrocholesterol synthesis in microorganisms. According to the application, the acetyl coenzyme A enhancing module comprises genes expressing ethanol dehydrogenase, genes expressing acetaldehyde dehydrogenase, genes expressing acetyl coenzyme A synthase and genes expressing ATP-citrate lyase. After the acetyl coenzyme A enhancing module is led in the microoranisms, the synthesis amount of the 7-dehydrocholesterol is remarkably increased. A new method is provided for synthesizing 7-dehydrocholesterol, and mass production of 7-dehydrocholesterol is facilitated better.
Owner:TIANJIN UNIV
A kind of synthetic method of 25-hydroxy-7-dehydrocholesterol
The present invention relates to a 25-hydroxy-7-dehydrocholesterol synthetic method, a 25-hydroxy-7-keto-cholesterol derivative is used as a raw material for mechanical milling reaction in a ball mill pot under the effect of sulfonyl hydrazide and a base in the absence of an organic solvent, and then hydrolysis reaction is performed to obtain 25-hydroxy-7-dehydrocholesterol synthetic method. According to the method, in the absence of a solvent, mechanical milling reaction is performed, no dust is spilled over in the grinding process, reaction controllability is good, reaction efficiency is high, the solvent recovery operation in the post-processing process can be avoided, operation is simplified, environmental pollution is reduced, the reaction selectivity is good, yield is high, operation is simple, cost is low, post-treatment is simple, three-waste is less, product is easy to separate, purity is high, the yield is up to 89%, the melting process is short, and the product purity is high.
Owner:ZHEJIANG UNIV OF TECH +2
Method for preparing 7-dehydrocholesterol
The invention relates to a method for preparing 7-dehydrocholesterol, which comprises the following steps: 1) protecting hydroxyl on the 3-site of cholesterol; 2) oxidizing carbon on the 7-site of the product obtained in step 1) into carbonyl; 3) carrying out a hydrazone reaction on the product obtained in step 2) and a hydrazine derivative; 4) reacting the product obtained in step 3) with a strongly alkaline reagent; and 5) reacting the product obtained in step 4) with water or an acid. The method has the advantages of simple operation process, less secondary reaction, high yield and less corrosion to equipment, thereby having high industrial production values.
Owner:ANHUI BBCA FERMENTATION TECH ENG RES
Clone and expression of 7,8-desaturase gene
InactiveCN101509004AShort process routeReduce pollutionMicroorganismsMicroorganism based processesCDNA libraryVitamin
The invention provides clone and expression aiming at 7,8-dehydrogenase gene. The gene encoding 7,8-dehydrogenase is acquired from a constructed drosophila cDNA library, wherein the 7,8-dehydrogenase can transform cholesterol into 7-dehydrocholesterol, thereby providing a new method for synthesizing the 7-dehydrocholesterol by a biological process, acquiring a process route capable of shortening vitamin D3, reducing production cost, and reducing environmental pollution.
Owner:GUANGXI UNIV
Production method of 7-dehydrocholesterol
The invention discloses a production method of 7-dehydrocholesterol. The method includes the steps: S1 dissolving cholesterol and benzoyl chloride in a solvent, and performing acylation reaction to obtain cholesteryl benzoate; S2 dissolving the cholesteryl benzoate and 1, 3-dibromo-5, 5-dimethyl hydantoin in the solvent, adding initiating agents, performing hydrazone reaction to obtain 7-bromo cholesterol benzoate; S3 dissolving the 7-bromo cholesterol benzoate and pyridine in ethyl alcohol, and performing hydrazone removal reaction to obtain 7-dehydrocholesterol benzoate; S4 adding the 7-dehydrocholesterol benzoate into sodium hydroxide solution, and performing saponification hydrolysis reaction to obtain the 7-dehydrocholesterol. The production method for the 7-dehydrocholesterol has the advantages of reasonable process, mild reaction condition, high selectivity, simplicity in operation, friendly environment and low production cost.
Owner:SICHUAN YUXIN PHARM CO LTD
Vitamin D3 preparation method and device
Owner:ZHEJIANG NHU CO LTD
Pegylated 7-dehydrocholesterol derivative
InactiveCN106170490AImprove stabilityLow toxicityCosmetic preparationsToilet preparationsWrinkle skinSolubility
The present invention relates to a PEGylated 7-dehydrocholesterol derivative, and a composition for wrinkle alleviation and antiaging, containing the same. The PEGylated 7-dehydrocholesterol derivative, according to the present invention, has improved water solubility and stability, and thus can be effectively used, as an excellent vitamin D source, in a cosmetic composition, a pharmaceutical composition, a dietary supplement and the like for wrinkle alleviation and antiaging.
Owner:HUMEDIX
Method for purifying 7-dehydrocholesterol leftovers
The invention discloses a method for purifying the intermediate 7-dehydrocholesterol leftovers of vitamin D3, which aims to solve the problem that the 7-dehydrocholesterol leftovers cannot be used and are discarded in industrial production due to the existence of a large amount of 3-hydroxycholestane-4,6-diene and the leftovers thereof to cause the high production cost of the vitamin D3 and environmental pollutions. The method comprises the following steps of: dissolving the 7-dehydrocholesterol leftovers containing the byproduct 3-hydroxycholestane-4,6-diene into an alcohol solvent in a volume which is twice to 10 times the weight of the leftovers, and stirring the mixture until the leftovers are completely dissolved; and adding an iron salt or aluminum salt into the system, continuing to perform stirring until the impurity 3-hydroxycholestane-4,6-diene is completely reacted, performing extraction on the obtained solution by using varsol, and then separating and removing the 3-hydroxycholestane-4,6-diene by using a chromatographic column. In the method, ether impurities produced in the reaction process can be easily separated from 7-dehydrocholesterol, so the treatment process is greatly reduced.
Owner:ZHEJIANG NHU CO LTD
7-dehydrocholesterol derivatives and methods using same
The present invention provides, in certain aspects, novel 7-dehydrocholesterol (7DHC) derivatives that are useful in treating or preventing cancer, as well as in treating or preventing uncontrolled angiogenesis, in a subject. In certain embodiments of the present invention, the subject is a human. In other aspects, the present invention provides a method of preparing compounds of the invention, or a salt or solvate thereof.
Owner:WOMEN & INFANTS HOSPITAL OF RHODE ISLAND
Chicken feed capable of improving egg yield
The invention discloses a chicken feed capable of improving egg yield. The chicken feed is prepared by the following steps: mixing 43-50 parts of corn, 22-26 parts of bean cake, 10-11 parts of wheat bran, 1 part of fish meal, 0.2 part of salt, 10 parts of oyster shell, 3 parts of sodium alga acid, 0.7 part of 7-dehydrocholesterol, 0.1 part of cholestanol, 0.4 part of sodium hexametaphosphate, 2.5 parts of an organic iron-zinc enzymic preparation, 0.1 part of pentaerythritol ester of rosin, 0.1 part of sodium potassium tartrate tetrahydrate and 0.1-0.3 part of sodium glutamate evenly; crushing, and drying to obtain the chicken feed. According to the obtained chicken feed, absorption of chicken and improvement of the egg yield of the chicken are facilitated; chicken can be prevented from being ill; and the chicken feed has the characteristics of wide raw material source and comprehensive nutrients.
Owner:PUTIAN SHANHAITIAN AGRI DEV
Vitamin D3 preparation method and device
The invention discloses a method for preparing vitamin D3 and used equipment. In the prior method, a solvent must be replaced during the crystallization process of the post-treatment, and the heating time is long, which affect the content of a final product. A solvent used by the method in a photochemical reaction is a mixed solvent consisting of a high melting point solvent and a low melting point solvent; an obtained photochemical reaction liquid is subjected to freezing crystallization and filtering post-treatment after the photochemical reaction is finished; and crude vitamin D3 oil is obtained after filtrate is concentrated; after a filter cake is heated and dissolved, 7-dehydrocholesterol and the low melting point solvent which is concentrated from the filtrate are added to serve as raw materials for the photochemical reaction; the high melting point solvent is alcoholic solvents or hydrocarbon solvents with melting points of between 10 DEG C below zero and 10 DEG C, and the low melting point solvent is alcohol solvents with melting points less than or equal to 50 DEG C below zero. The vitamin D3 obtained by the method has high content and less polymerization side reactions, does not need to replace the solvent during the post-treatment process, and has good quality.
Owner:ZHEJIANG NHU CO LTD
Compositions for the treatment of cataracts
ActiveUS20170027961A1Reduce formationIncreased lens clarityOrganic active ingredientsSenses disorderCholestadienolsPharmacology
In one embodiment, the present application discloses an aqueous ophthalmic composition for the treatment of eye diseases, lesions and injuries, comprising: a) one steroid, or a combination of at least two steroids selected from the group consisting of lanosterol, dihydrolanosterol, 4,4-dimethylcholesta-8(9),14,24-trien-3β-ol, 4,4-dimethylcholesta-8,24-dien-3β-ol, 4,4-dimethylcholesta-8-en-3β-ol, 4,4-dimethylcholesta-8(9),14-dien-3β-ol, 14-desmethyl lanosterol, lathosterol, Δ7,24-cholestadienol, cholesterol, cholesta-7-enol, cholesteryl ester, 7-dehydrocholesterol, desmosterol, 7-dehydrodesmosterol, zymosterol, 27-hydroxycholesterol, cholesta-7,24-dien-3-β-ol, cholesta-8(9)-en-3-β-ol, 5α-cholestan-3β-ol-6-one, 5-cholesten-3β,25-diol, 5-cholesten-3β,25-OSO3H (5-cholesten-3β,25-sulfate), 5-cholesten-3β-OSO3H,25-ol (5-cholesten-3β-sulfate,25-ol), 5-cholesten-3β,25-diol, disulfate, and their esters thereof, or a pharmaceutically acceptable salt thereof, in a concentration effective for the treatment and / or prophylaxis of the eye diseases, lesions and injuries; and b) a pharmaceutical excipient; and methods of treatment using such compositions.
Owner:CATACORE INC
Prawn Y-organ 7,8-dehydrogenase gene and application thereof
InactiveCN104561051AEnzymatic hydrolysis is simple and efficientShort process routeMicroorganism based processesOxidoreductasesBiotechnologyCDNA library
The invention belongs to the field of gene engineering, and particularly relates to a prawn Y-organ 7,8-dehydrogenase gene and application thereof. The invention provides a gene for coding 7,8-dehydrogenase, which is obtained from a constructed Metapenaeus ensis Y-organ cDNA (complementary deoxyribonucleic acid) library; and the nucleotide sequence of the gene is disclosed as SEQ ID NO.1. The application method comprises the following steps: cloning the 7,8-dehydrogenase coding gene, and connecting with an expression plasmid to construct a recombinant expression plasmid; after linearizing and purifying the recombinant expression plasmid, converting a receptor bacterium, and screening to obtain a gene engineering bacterium; and carrying out induction expression, purification and identification to obtain the 7,8-dehydrogenase of which the amino acid sequence is disclosed as SEQ ID NO.2. The 7,8-dehydrogenase can be used for converting cholesterol into 7-dehydrocholesterol by a one-step enzyme process, thereby enhancing the conversion efficiency, shortening the process route for producing vitamin D3, lowering the production cost and reducing the environmental pollution.
Owner:GUANGXI UNIV
Enzymatic method to produce 7-dehydropregnenolone, vitamin D3-like compounds and derivatives thereof
Provided herein are enzymatic methods of producing steroid compounds such as 7-dehydropregnenolone, hydroxy derivatives thereof and vitamin D2- and D3-like compounds and derivatives thereof. Also provided are the derivatives of the vitamin D3-like compounds so generated via the action of cytochrome P450scc enzyme on substrates 7-dehydrocholesterol, vitamin D2 or vitamin D3.
Owner:UNIV OF TENNESSEE RES FOUND
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