Method utilizing microsomal enzyme of animal liver to biosynthesize 7-dehydrocholesterol

A technology for dehydrocholesterol and biosynthesis, which is applied in the fields of biochemical engineering and enzyme engineering, can solve the problems of low efficiency, difficult control of conditions, affecting the production cost of vitamin D, and marketing promotion of sales price, so as to reduce environmental pollution and reduce production costs. Effect
CN101575633AInactive Publication Date: 2009-11-11GUANGXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGXI UNIV
Publication Date
2009-11-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of biochemical engineering and enzyme engineering, and in particular relates to a method utilizing the microsomal enzyme of an animal liver to biosynthesize 7-dehydrocholesterol. The method includes the following steps: (1) preparation of microsomal enzyme solution; (2) enzymatic conversion reaction; (3) saponification reaction; and (4) extraction. The method can prepare the 7-dehydrocholesterol which is completely identical with that prepared by chemosynthesis; and meanwhile, the technology is simple and easy, and not only the production cost of vitamin D3 can be reduced, but also the environment pollution can be reduced.
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Description

technical field

[0001] The invention belongs to the technical fields of biochemical engineering and enzyme engineering. It specifically relates to a method for synthesizing 7-dehydrocholesterol by using animal liver microsomal enzymes Background technique

[0002] Vitamin D 3 It is an essential fat-soluble vitamin for the growth, development, reproduction, maintenance of life and health of humans and animals. Its main function is to regulate calcium and phosphorus metabolism, promote intestinal calcium and phosphorus absorption and bone calcification, maintain the balance of blood calcium and blood phosphorus, and can be used as pharmaceutical preparations, food and feed additives, etc., and can also be used clinically to treat rickets , senile osteoporosis, hypothyroidism, etc. current vitamin D 3 It is produced by photochemical method, that is, 7-dehydrocholesterol (7-DHC) is used as raw material, which is converted into vitamin D after ultraviolet irradiation 3 Precu...

Claims

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