Identification method for light-induction root color reddening rice
An identification method and light-induced technology, applied in the field of molecular biology, to achieve the effects of convenient operation, high breeding efficiency and strong pertinence
Inactive Publication Date: 2012-05-30
SHANGHAI NORMAL UNIVERSITY
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Problems solved by technology
In order to explore the genetic mechanism of anthocyanin expression in rice roots and make up for the defect that the roots of all rice varieties currently used in production do not contain anthocyanins, it is urgent to solve the problem of breeding a new rice variety with high anthocyanin roots Methods
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[0017] The Thai indica rice variety Khao Nok was crossed with the mutant HG1, and the F 2 20 seeds were randomly selected from each plant and put into a petri dish, and placed in a light incubator [12h per day 180μmol·m -2 ·s -2 Light intensity treatment, light culture temperature is 28 ℃, and dark culture temperature is 25 ℃], cultivate 20 days with double distilled water, investigate the separation situation of root color, press F then 3 After the root color of the seedlings is classified, the recessive white root seedlings are moved into seedling trays for planting. Randomly select 10 plants to form a gene pool to extract the DNA of the white root seedlings, and use the molecular marker provided by the present invention to detect the light-induced red root of rice. The nucleotide sequence of the molecularly marked specific PCR primer from the 5' end to the 3' end of the upstream end is CGTCCATTCACAGGGTA, and the nucleotide sequence of the downstream end from the 5' end to...
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The invention discloses a breeding method of rice, the root of which becomes to be red when being processed by photoinduction. The method comprises special PCR primer design, extraction of DNA, PCR, endonuclease reaction and testing process; wherein, the nucleotide sequence from upstream end 5'-end 3' of the special PCR primer is CGTCCATTCACAGGGTA, and the nucleotide sequence from downstream end 5'-end 3' is CAGCAGATGAGGCAAACT. The special PCR primer designed by the invention can be used for amplifying DNA extracted in rice seedling stage, and amplified production can be identified by CAPS1 molecular marker after zymolysis by BamH I. Therefore, the seed selection method can rapidly and accurately select the novel rice having the DNA leading the root to become to be red when being processed by photoinduction, thus overcoming the defect that the existing breeding method has blindness and low efficiency, and having the advantages of high breading efficiency, strong direction and convenient operation.
Description
technical field [0001] The invention relates to a method for breeding rice with light-induced reddened root color, and belongs to the technical field of molecular biology. Background technique [0002] Anthocyanins belong to flavonoid compounds, which not only exhibit excellent color, but also have physiological functions such as anti-oxidation or anti-lipid oxidation activity, anti-inflammatory activity, lowering serum cholesterol and serum lipids, inhibiting cancer cells and anti-cancer. As an important biologically active substance in rice, anthocyanins have become one of the hotspots in the development and research of functional foods. Actively cultivating high-quality rice varieties with high anthocyanin content can not only provide a theoretical basis for rice breeding and related gene cloning with high anthocyanin content, but also meet people's consumption demand for functional rice, which is conducive to the development of domestic and foreign rice markets. The foo...
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IPC IPC(8): C12Q1/68
Inventor 张俊芝张永娟林冬枝董彦君
Owner SHANGHAI NORMAL UNIVERSITY