Human embryonic stem cell line with nonrandom inactivated X chromosome and specific short tandem repeats

Abstract

The invention relates to a human embryonic stem cell line with nonrandom inactivated X chromosome and specific short tandem repeats, which is characterized in that the average population doubling time of the embryonic stem cell line is about 32 hours; the undifferentiated clone AKP of the embryonic stem cell line is detected to be strongly positive and has higher alkali phosphatase activity; the embryonic stem cell line can express specific surface antigens SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, but does not express a surface antigen SSEA-1; the embryonic stem cell line can express an Oct-4 transcription factor and keep undifferentiated characteristics; the embryonic stem cell line carries out once karyotype analysis every 10 generations, and has normal karyotype or translocation 13 trisomes; the embryonic stem cell line has two X chromosomes, and the inactivation analysis of the X chromosomes displays the nonrandom inactivation characteristics thereof; and the embryonic stem cell line has the specific short tandem repeats.

Description

technical field [0001] The invention relates to human embryonic stem cells derived from fertilized eggs, in particular to a human embryonic stem cell line with non-randomly inactivated X chromosomes and specific short tandem repeat sequences. Background technique [0002] Human embryonic stem cells (hES) are isolated and cultured from the inner cell mass (ICM) of blastocysts. They are cells with totipotent differentiation characteristics, self-renewal and multi-lineage differentiation potential. It is of great significance in the fields of therapy, embryonic development, drug screening, gene therapy, and research on genetic and epigenetic mechanisms. Human embryonic stem cell research began in 1998, and it has become the most active research field in life science after the Human Genome Project. In recent years, breakthroughs have been made in the isolation and purification, performance research, culture expansion, and induced differentiation of embryonic stem cells. In 199...

AI Technical Summary

Problems solved by technology

[0018] The selection and initiation of X chromosome inactivation occurs at the blastocyst stage. This process is controlled by the X inactivation center (XIC). XIC is a cis-acting site that contains information to identify the number of X chromosomes and the XIST gene. One chromosome is active, but the mechanism is unknown, the loss of XIST gene will lead to the failure of X chromosome inactivation

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