Method for extracting ursolic acid from vegetable Dian clove

A technology of Yunnan clove and ursolic acid, which is applied in the field of extraction and separation of ursolic acid, can solve the problems of high cost, difficult treatment, and large amount of "three wastes" treatment

Inactive Publication Date: 2011-06-08
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, ursolic acid is synthesized by chemical methods, and there are "three wastes" in the chemical synthesis, which has a large amount of treatment and is difficult to deal with, and it is easy to pollute the environment and the cost is high
So far, there are many reports about extracting and isolating ursolic acid from other plants, but most of them are extracted from Oleaceae plants and rhododendron plants, and there is no report on obtaining ursolic acid from the Rubiaceae plant Dian lilac.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Take 20kg of dried Lilac yunnanensis, pulverize, cold-soak with 10L of 95% industrial ethanol for 3 times, each time for 7 days, filter, recover the ethanol under reduced pressure to obtain a concentrated solution, disperse the concentrated extract in 2L of water, and wash with ethyl acetate successively, N-butanol was extracted three times each, and the ethyl acetate extract was subjected to 200-300 mesh silica gel medium-pressure column chromatography, eluted with petroleum ether-acetone solvent (volume ratio: 30:1), and detected by thin-layer chromatography as a reference standard , The parts of the compound were combined and subjected to Sephadex LH-20 gel column chromatography and eluted with methanol to obtain the pure product of the compound.

Embodiment 2

[0013] Pretreatment of macroporous resin: Pack D101 resin column, soak in 5% NaOH for 2 hours, elute 2 column volumes at a flow rate of 4 column volumes per hour, then wash with distilled water until the pH value is neutral; add ethanol and soak for 1 hour , eluted for 3.5 column volumes at a flow rate of 2 column volumes per hour, and finally washed with distilled water until no alcohol smell. Preparation of the sample solution: take 20 kg of dried Dian cloves, after pulverization, cold soak 3 times with 95% industrial ethanol, each time for 7 days, filter, reclaim the ethanol under reduced pressure to obtain a concentrated solution, add water to a suitable concentration according to 1:30 (extract weight ratio with macroporous resin); sample solution is added to resin column, water-ethanol solvent gradient elution, 60% ethanol water part is concentrated into extractum, carries out Sephadex LH-20 gel column chromatography, is eluent with methanol , standard control, isolated p...

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Abstract

The invention relates to a method for extracting ursolic acid from vegetable Dian clove. The method comprises the following steps that: (1) the dried Dian clove is picked up, crushed and undergoes repeated cold soaking by 10L of 95 percent ethanol for three times, seven days for each time, and the ethanol is filtered, decompressed and reclaimed to obtain a concentrated extractive; (2) the obtained ethanol extractive is tested; and (3) the extract obtained from the step(2) is subjected to Sephadex LH-20 gel column chromatography and is separated to obtain the finished product by taking methanol as an eluant and compare with the standard product. The separation materials used by the method include macroporous adsorptive resin and gel, so that the separation materials have stable physical-chemical properties, are not dissolved in acids, alkalis and organic dissolvants, have good selectivity on organic matters, and are not affected by existence of organic salts and strong-ion low-moleculecompounds; and the separation materials are easily eluted and regenerated, thereby facilitating the cyclic utilization.

Description

technical field [0001] The invention relates to a method for extracting ursolic acid from plants, in particular to a method for extracting and separating ursolic acid from Rubiaceae plant Lilac yunnanensis. Background technique [0002] Ursolic acid (ursolic acid, UA), also known as ursolic acid, is widely distributed in nature. It exists in the leaves and fruits of the rhododendron bearberry plant, the leaves of the plant Paulownia tomentosa, and the fruit of the Rubiaceae plant Gardenia , Gentianaceae wet buds, Oleaceae privet leaves and other plants. Ursolic acid has a wide range of biological effects, and was listed as one of the most promising tumor prevention drugs by Japan in 1990. It has obvious stabilizing and cooling effects on the central nervous system; it has antibacterial activity against G+ and G- bacteria and yeast in vitro; it has anti-diabetic, anti-ulcer and blood-lipid-lowering effects; it is resistant to various carcinogens and carcinogens Function; it...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00A61K36/74
Inventor 康文艺李昌勤王天晓魏金凤王金梅宋艳丽姬志强
Owner HENAN UNIVERSITY
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