Frozen semen diluent and preparation method thereof

A technology of freezing semen and diluent, which is applied in the preservation, application, and animal husbandry of human or animal bodies. It can solve the problems of low vitality, low fertilization rate, and artificial insemination, and achieve the effect of improving vitality and quality

Active Publication Date: 2012-05-09
NORTHWEST A & F UNIV
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AI Technical Summary

Problems solved by technology

However, in production practice, there are often cases of low vitality and low fertilization rate after freezing and thawing sperm, which affects the effect of artificial insemination

Method used

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Comparison scheme
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Embodiment Construction

[0038] According to the technical scheme of the present invention, the substances required to prepare 100 ml of frozen semen diluent are: sodium citrate: 0.9-1.2 g, sucrose: 3-5 g, egg yolk: 18-22 ml, glycerin: 4-7 ml, Vitamin E: 6-9 mg, vitamin C: 500-700 mg, spectinomycin: 35 mg, gentamicin: 80,000 units (IU).

[0039] Its optimal frozen semen diluent formula is: the substance content per 100ml is: sodium citrate: 1.073mg, sucrose: 4.344mg, egg yolk: 20ml, glycerin: 6ml, vitamin E: 8mg, vitamin C: 600 mg, spectinomycin: 35 mg, gentamicin 80,000 units (IU).

[0040] Its preparation method is:

[0041] 1) Weigh the raw materials in the formula: sodium citrate, sucrose, egg yolk, glycerin, vitamin E, vitamin C, distilled water.

[0042] 2) The above sodium citrate, sucrose, egg yolk, vitamin C, vitamin E, and glycerin were prepared into a base solution with distilled water, and stirred with a magnetic stirrer for 60 minutes to obtain a mixed solution, which was stored overnig...

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Abstract

The invention discloses a novel frozen semen diluent used for improving the freeze-thaw quality and fertilization rate of frozen bovine semen and a preparation method thereof. Every 100ml of the prepared frozen semen diluent contains 0.9-1.2g of sodium citrate, 3-5g of sucrose, 18-22ml of yelk, 4-7ml of glycerin, 6-9mg of vitamin E, 500-700mg of vitamin C, 35mg of spectinomycin and 80 thousand units of gentamicin. The invention adds VE and VC into the semen diluent for the first time, so as to increase the motility rate and acrosome integrity of the frozen sperm as well as the activity of antioxidant enzyme in seminal plasma, thus improving the quality and fertilization rate of the freeze-thaw sperm.

Description

technical field [0001] The invention belongs to the improved technical category of bovine artificial insemination, in particular to a frozen semen diluent used for bovine frozen semen to improve the frozen quality of bovine semen and a preparation method thereof. Background technique [0002] The successful freezing of livestock semen has had an extremely far-reaching impact on the development of artificial insemination technology. First, it has laid a foundation for expanding the utilization rate and prolonging the utilization time of excellent breeding males, and secondly, it provides a broad field for the development of artificial insemination and cryobiology. space. Because the frozen semen is more convenient in transportation, both in terms of time and space, the breeding efficiency of males can be brought into full play, which accelerates the pace of livestock breed improvement. Although various countries have carried out research on frozen semen of cattle, pigs, hors...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 昝林森赵宪林刘永峰胡建宏田万强孙永刚桂林生
Owner NORTHWEST A & F UNIV
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