Gene chip of prediction of recurrence after hepatocellular carcinoma operation

A technology of hepatocellular carcinoma and gene chip, which is applied in the field of gene chip for predicting recurrence of hepatocellular carcinoma after operation, can solve problems such as unreported, and achieve the effects of easy popularization and application, simple method, and accurate diagnosis and prediction.

Inactive Publication Date: 2009-10-21
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

So far, similar studies on screening prognosis-related genes from differential gene expression profiles of tumor stem-like cells in liver cancer have not been reported at home and abroad

Method used

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  • Gene chip of prediction of recurrence after hepatocellular carcinoma operation
  • Gene chip of prediction of recurrence after hepatocellular carcinoma operation
  • Gene chip of prediction of recurrence after hepatocellular carcinoma operation

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Embodiment

[0016] A gene chip for predicting postoperative recurrence of hepatocellular carcinoma, with EPAS1, JMJD1C, NEDD9, EPC2 and USP36 genes as detection sites, β-actin as internal reference, and the primers shown in the following table as detection probes. A copy of the reading form is attached:

[0017] gene name

Upstream and downstream primer sequences

size

EPAS1

F: 5'TGCTACGCCACCCAGTACCA3'

R: 5'CAGTTCGGGCAGCAGGTAGG3'

111bp

JMJD1C

F: 5'CAGCGGGAGCACTTCATCATCAG3'

R: 5'TTCCTTCAAAAGTCTCAGTTCCTGTG3'

125bp

NEDD9

F: 5'CCCAAACCGAAGTCATAGCC3'

R: 5'CAGGAACAGCCACCAATAAGC3'

154bp

USP36

F: 5'CGAAGAGTTTGACCGAGGGAAG3'

R: 5'GCAGCCTTTGCTGGGTGAGT3'

132bp

EPC2

F: 5'CATTTGGGAGACTATGGTGGT3'

R: 5'CTCTGCTTTAGGCTTCTTTGG3'

213bp

β-actin

F: 5'AAGAAGGTGGTGAAGCAGGC 3'

...

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Abstract

The invention provides a gene chip of prediction of recurrence after hepatocellular carcinoma operation. The invention is characterized in that the chip takes EPAS1, JMJD1C, NEDD9, EPC2 and USP36 genes as detecting sites, takes beta-actin as the actin antibody and takes upstream primers and downstream primers on the EPAS1, JMJD1C, NEDD9, EPC2 and USP36 genes as detecting probes. The gene chip has the characteristics of accurate and fast diagnosis and prediction, simple method and easy promotion and application and the like. The gene can be used for clinically guiding adjuvant therapy after the operation of liver cancer, and reduce recurrence rate of high-risk patients with liver cancer after operation, thus improving the prognosis of patients with liver cancer. The group of gene labels has important clinical significance to the judgment of prognosis of patients with feminine alpha-fetoprotein and carcinoma in the early stage.

Description

technical field [0001] The invention relates to a gene chip for predicting postoperative recurrence of hepatocellular carcinoma, belonging to the field of gene technology. Background technique [0002] my country is a high-incidence area of ​​liver cancer in the world. About 200,000 people die of liver cancer every year, accounting for about half of the global liver cancer deaths. Modern medicine has conducted scientific research on liver cancer for more than 100 years. Although many achievements have been made, the overall 5-year survival rate of liver cancer patients is still only about 3%. The biggest obstacle to improving the prognosis of liver cancer is its extremely high recurrence rate. Even with radical surgical resection, 50% of patients will develop metastasis or recurrence within 5 years. Therefore, predicting postoperative recurrence of liver cancer patients for early necessary intervention is the key to further improve the prognosis of liver cancer. Although t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B40/06
Inventor 樊嘉徐泱杨欣荣周俭邱双健
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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