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Aspergillus fumigatus and application thereof

A technology of Aspergillus fumigatus and podophyllotoxin, which is applied in the directions of microorganisms, fungi, biochemical equipment and methods, etc., can solve the problems of difficult large-scale artificial cultivation, complex chemical structure, long growth cycle, etc., and achieves protection of plant ecology. The effect of diversity, simple fermentation conditions and short production cycle

Inactive Publication Date: 2009-11-11
BEIJING NORMAL UNIVERSITY
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] Due to the complex growth environment of plants, it is difficult to simulate, and its growth cycle is long, it is difficult to carry out large-scale artificial cultivation, and tissue culture technology is not suitable for podophyllum plants; in addition, the chemical structure of podophyllotoxins is complex, chemical synthesis Although it can be carried out, the cost is too high, and the construction of engineered bacteria by means of genetic engineering cannot be carried out due to the large number of enzymes involved in the formation of such substances.

Method used

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  • Aspergillus fumigatus and application thereof
  • Aspergillus fumigatus and application thereof
  • Aspergillus fumigatus and application thereof

Examples

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Embodiment 1

[0032] Example 1. Isolation and identification of Aspergillus fumigatus (Aspergillus fumigatus) TEQA CGMCC №3115

[0033] The roots and rhizomes of the plant Sinopodophyllumhexandrum (Royle) Ying collected from Dacaotan (2700m above sea level) in Zhangxian County, Gansu Province were washed with tap water respectively, and cleaned with 75% volume percentage content of Treat with ethanol for 5 minutes, then rinse with sterile water for 3-5 times; then treat with 2.5% by mass sodium hypochlorite for 10 minutes, and then rinse with sterile water for 3-5 times. Use sterilized tweezers and razor blades to peel off the outer skin of the root of P. chinensis, then cut into small pieces with a size of 0.5cm×0.5cm and plant them on PDA solid medium, and cultivate them at 28°C for 3-7 days. At the same time, the roots of the same sterilized P. chinensis were not peeled and cut, and the roots of P. chinensis were rolled on the PDA medium for one week, and cultured under the same conditio...

Embodiment 2

[0038] Example 2. Preparation of Podophyllotoxin, Epipodophyllotoxin Diglucoside and Astragaloside by Aspergillus fumigatus (Aspergillus fumigatus) TEQA CGMCC №3115

[0039] The composition of potato dextrose liquid culture medium is: 200g of peeled potatoes, 20g of glucose, add water and dilute to 1000mL.

[0040] The preparation method is as follows: Peel the potatoes and cut them into about 2cm 2 put into a beaker and boil for 30 minutes, then filter with double-layer gauze, take the filtrate and add glucose, then make up water, the pH is natural.

[0041] 100ml of the prepared potato dextrose liquid medium was packed in a 250ml Erlenmeyer flask, and sterilized for later use. Pick a single colony of Aspergillus fumigatus (Aspergillus fumigatus) TEQA CGMCC №3115 screened in Example 1 above and inoculate it in the above-mentioned sterilized potato dextrose liquid medium, and cultivate it on a shaker at 28° C. and 120 r / min for 7 days.

[0042] The above-mentioned fermentati...

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Abstract

The invention discloses an aspergillus fumigatus which can produce podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside, and application thereof. The aspergillus fumigatus TEQA CGMCC No. 3115 has the function of producing podophyllotoxin, epipodophyllotoxin biglucoside and flavonoids astragaloside which have the physiological property the same as that of Chinese podophyllum root. The aspergillus fumigatus can be used for preparing podophyllotoxin and astragaloside. The method for preparing podophyllotoxin, epipodophyllotoxin biglucoside and astragaloside has the advantages of simple fermentation conditions, easy bacterium culture and short production period, with potential application for industrialized mass production. Besides, the aspergillus fumigatus provides a new way for resource development of podophyllotoxin and has important meaning to protect ecological diversity of plants.

Description

technical field [0001] The invention relates to a strain of Aspergillus fumigatus and its application. Background technique [0002] Podophyllotoxin (podophyllotoxin) mainly exists in the Berberidaceae Podophyllum and its related plants. It is a class of natural compounds with a wide range of biological activities, including estrogen-like, anti-estrogen-like, anti-cancer, anti-viral , antifungal, sedative, inhibiting plant growth and antioxidant effects. In particular, it has strong anticancer activity, and its mechanism of action is to inhibit the depolymerization of tubulin to prevent cell division and inhibit the activity of DNA topoisomerase. The glycoside derivatives of podophyllotoxin, etoposide and teniposide, have low toxicity and have good curative effects on various tumor diseases such as small cell lung cancer and lymphoma, so they have become broad-spectrum anti-tumor drugs for clinical application. [0003] At present, podophyllotoxin is mainly extracted from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C12P19/60C12R1/68
Inventor 赵长琦贾栗朱妍妍艾嫦
Owner BEIJING NORMAL UNIVERSITY
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