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Process for the preparation of hollow cellulose vessels by culturing cellulose- producing microorganisms on the surface of a hollow carrier and providing a gas having an oxygen level of at least 35%

A technology of oxygen level and microbial cellulose, which is applied in the field of hollow cellulose tubes, can solve the problems of low blasting pressure, poor mechanical properties, and unsuitability, and achieve good mechanical properties and shorten the cultivation period.

Inactive Publication Date: 2009-12-02
ARTERION AB
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This approach yields microbial cellulose with a horizontally laminated structure that is less suitable as a substitute for larger blood vessels due to poor mechanical properties (e.g. low burst pressure)

Method used

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  • Process for the preparation of hollow cellulose vessels by culturing cellulose- producing microorganisms on the surface of a hollow carrier and providing a gas having an oxygen level of at least 35%
  • Process for the preparation of hollow cellulose vessels by culturing cellulose- producing microorganisms on the surface of a hollow carrier and providing a gas having an oxygen level of at least 35%
  • Process for the preparation of hollow cellulose vessels by culturing cellulose- producing microorganisms on the surface of a hollow carrier and providing a gas having an oxygen level of at least 35%

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Embodiment Construction

[0019] One embodiment of the present invention is an improved method of producing hollow microbial cellulose by culturing cellulose-producing microorganisms on the outer surface of a hollow carrier. An oxygen-containing gas is supplied to the inside of the hollow carrier.

[0020] The method is further characterized in that the oxygen-containing gas has an oxygen level higher than atmospheric oxygen. Preferably, at oxygen levels between 21%-100%, 35%-100%, 50%-100%, 60%-100%, 70%-100%, 80%-100% or 90%-100% cultivated in between. The remainder of the gas used may be any inert gas such as nitrogen, argon, helium. Oxygen percentages are expressed as v / v percentages.

[0021] Preferably, cultivation is performed at an oxygen level of 100%.

[0022] To further increase the partial pressure of oxygen, the gas may be provided at a pressure higher than atmospheric pressure, for example at a pressure of 0.2, 0.5, 1.0, 2.0 or 5.0 bar above atmospheric pressure.

[0023] Preferably,...

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Abstract

The present invention relates to an improved method for the preparation of hollow cellulose vessels produced by a microorganism, and hollow cellulose vessels prepared by this method. The method is characterized by the culturing of the cellulose-producing microorganisms being performed on the outer surface of a hollow carrier, and providing an oxygen containing gas on the inner side of the hollow carrier, the oxygen containing gas having an oxygen level higher than atmospheric oxygen. The hollow microbial cellulose vessels of the present invention are characterized by improved mechanical properties and can be used in surgical procedures to replace or repair an internal hollow organ such as the urethra, ureter, the trachea, a digestive tract, a lymphatic vessel or a blood vessel.

Description

technical field [0001] The invention relates to an improved preparation method of a hollow cellulose tube produced by microorganisms, and the hollow cellulose tube prepared by the method. The hollow microbial cellulose tube of the present invention can be used as a substitute for hollow organs in the body such as ureter, trachea, digestive tract, lymphatic or blood vessels. Background technique [0002] It is already known, for example, from JP 3 165 774 A1 that cellulose produced by microorganisms (hereinafter referred to as "microbial cellulose") can be used as biomaterial in surgical applications, for example as abdominal wall, skin, subcutaneous tissue, organs, digestive Tissue implants for trachea, esophagus, trachea and urethra as well as cartilage tissue and lipoplastic surgery. In addition, it is known (eg from JP 8 126 697 A2, EP 186 495 A2, JP 63 205 109 A1, JP 3 165 774 A1) that during the production of microbial cellulose it can be molded into specific shapes fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08L1/02A61L27/20
CPCA61L27/507A61L27/3808A61L27/20C12P19/04C08L1/02Y10T428/13Y02P20/582
Inventor A·博丁H·巴克达尔P·盖腾霍尔姆L·古斯塔夫桑B·里斯伯格
Owner ARTERION AB
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