Method for creating and evaluating disease and syndrome combined animal models

Abstract

The invention provides a preparation method and an evaluation system of animal models of syndrome of blood stasis caused by myocardial ischemia and syndrome of blood stasis caused by myocardial ischemia and deficiency of vital energy, belonging to the technical field of research on syndrome of traditional Chinese medicine for preparing and evaluating disease and syndrome combined animal models. The animal model of syndrome of blood stasis caused by myocardial ischemia is created by putting an Ameroid constriction ring in the coronary artery. 1 to 8 weeks later after operating on an animal pattern, the processes of dynamic observation, physicochemical index detection, electrocardiogram, ultrasonic cardiogram and coronary radiography are carried out, and 4 weeks after the operation, the animal pattern accords with the diagnosis of syndrome of blood stasis caused by myocardial ischemia. The animal model of syndrome of blood stasis caused by myocardial ischemia and deficiency of vital energy is prepared by ligating left coronary artery. 30 days later after operating on another animal pattern, the processes of dynamic observation, electrocardiogram, ultrasonic cardiogram and pathology detection are carried out, and about 7 days after the operation, the animal pattern is found to accord with the diagnosis of syndrome of blood stasis caused by myocardial ischemia and deficiency of vital energy and still support the diagnosis of syndrome of blood stasis caused by myocardial ischemia until 30 days before material drawing.

Description

technical field [0001] The invention belongs to the technical field of TCM syndrome research, and specifically relates to a preparation method and evaluation system construction of disease-syndrome-combined animal models, and is a preparation method and evaluation system for animal models of myocardial ischemia and blood stasis syndrome and myocardial ischemia-qi-deficiency and blood-stasis syndrome. Background technique [0002] At present, there are five main ideas for the development of TCM syndrome models: (1) etiology modeling; (2) drug modeling; (3) pathology modeling; (4) etiology and pathology modeling; (5) combination of disease and syndrome modeling . All existing ideas have their limitations. For example, the etiology modeling only considers one or some factors of syndrome occurrence, but ignores the dynamics and time and space of syndromes, and cannot objectively and successfully reproduce the syndrome model at one time; another example is drug modeling. Syndro...

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