Immunochromatography test kit using novel whole blood sample pad processing method

A technology for immunochromatographic detection and whole blood samples, which is applied to measurement devices, analytical materials, instruments, etc., can solve the problems of complex production process, difficult to master, large difference in the quality of goat anti-erythrocyte serum, easy failure, etc., and prolong the storage time. , The effect of small sample volume and low cost

Inactive Publication Date: 2009-12-30
BEIJING BGI GBI BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the advantages of small amount of sample and low price, but the quality of goat anti-erythrocyte serum from differe

Method used

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  • Immunochromatography test kit using novel whole blood sample pad processing method
  • Immunochromatography test kit using novel whole blood sample pad processing method
  • Immunochromatography test kit using novel whole blood sample pad processing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Using kaolin-cephalin suspension to process whole blood sample pad to prepare colloidal gold immunochromatographic detection kit for hepatitis B surface antigen

[0029] 1. Prepare kaolin-cephalin suspension (containing 24g / l kaolin, 2g / l cephalin, 0.25mmol / l CaCl 2 );

[0030] 2. Completely immerse the polyester film in the suspension, take it out and dry it to get the whole blood sample pad.

[0031] 3. Preparation of colloidal gold particles: take 0.01% HAuCl 4 Solution 50ml, heat to boil, then quickly add 0.5ml of 1% trisodium citrate solution; continue to boil for about 5 minutes to end the reaction, the solution turns cherry red, make colloidal gold solution, cool and store.

[0032] 4. Labeling antibody and solidification: the colloidal gold solution and the mouse anti-HBsAg monoclonal antibody were mixed with 0.1mol / l K 2 CO 3 Adjust the pH value to 8.2, electromagnetically stir the colloidal gold solution, add mouse anti-HBsAg monoclonal antibody...

Embodiment 2

[0042] Example 2: Preparation of Colloidal Gold Immunochromatographic Detection Kit for Hepatitis B Surface Antigen by Using Ellagic Acid-cephalin Suspension to Treat Whole Blood Sample Pad

[0043] 1. Preparation solution (2g / L containing ellagic acid, 3g / L cephalin, 0.25mmol / L CaCl 2 );

[0044] 2. Fully immerse the glass cellulose membrane in the solution, take it out and spread it flat and dry it to make a whole blood sample pad.

[0045] 3-7. Same as steps 3-7 of Example 1.

[0046] 8. Result evaluation:

[0047] Use this test strip to detect hepatitis B surface antigen positive whole blood / serum hepatitis B surface antigen negative whole blood / serum, and quality control serum in clinical inspection centers. Observe the results and compare them with the products on the market. The results are shown in Table 2.

[0048] Table 2 Treatment of whole blood sample pads with ellagic acid-cephalin

[0049] Preparation of Hepatitis B Surface Antigen Immunochromatographic Dete...

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Abstract

The invention discloses an immunochromatography test kit using a novel whole blood sample pad processing method and relates to a novel whole blood sample pad processing method, an immunochromatography kit prepared by using the same, and a test method of the immunochromatography kit. The whole blood sample pad of the invention can be realized by uniformly spreading a non-bioactive prothrombin activator and solution containing phospholipids and calcuium ions Ca<2+> on a sample pad and can be widely used in immunochromatography test kits capable of synchronously testing whole blood/ serum/plasma samples. The method can make the preparation and production process of immunochromatographic reagents using whole blood as a test sample simple, convenient and effective and has the advantages of low cost, small sample dosage, high test speed and the like at the same time.

Description

technical field [0001] The invention relates to an immunochromatography detection kit using a novel whole blood sample pad processing method, relates to a novel whole blood sample pad processing method, an immunochromatography kit prepared by this technology and a detection method thereof, which are low in cost and easy to operate , rapid diagnosis, safe and reliable, and can be widely used in immunochromatographic detection kits for simultaneous detection of whole blood / serum / plasma samples. technical background [0002] In order to meet the requirements of simultaneous detection of human or animal whole blood and serum samples, the rapid immunological detection reagent generally adopts a polyester material with a certain pore size attached to the sample pad to realize the separation and detection of blood cells and serum at the same time. Its working principle is that when the blood cell volume is greater than or equal to the pore size of the polyester material and is filt...

Claims

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Application Information

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IPC IPC(8): G01N33/543
Inventor 李欣李争王宇帆
Owner BEIJING BGI GBI BIOTECH
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