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Method for extracting and purifying medical polysaccharide of large leaf moss

A technology of huixincao and polysaccharides, which is applied in the direction of drug combination, cardiovascular system diseases, etc., can solve the problems of less huixincao and no extraction of huixincao polysaccharides, and achieve the effect of safe, non-toxic and high yield

Inactive Publication Date: 2012-05-30
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is a pity that at present there are few studies on Herba huixincao at home and abroad, and they are mainly studied from the perspectives of pharmacognosy and overall clinical pharmacology, and there are few research reports on other aspects. to report

Method used

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  • Method for extracting and purifying medical polysaccharide of large leaf moss
  • Method for extracting and purifying medical polysaccharide of large leaf moss
  • Method for extracting and purifying medical polysaccharide of large leaf moss

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Investigate the effect of extraction time on the extraction rate: crush the Herba Sinensis, pass through 60 meshes, put in an ultrasonic extractor, add 20 times of water, adjust the pH to 8-9, raise the temperature to 90°C, and extract separately Three extracts were combined at different times. After centrifuging for 15 minutes, take the clear liquid; concentrate the extract to a certain volume under reduced pressure, remove the protein by savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, dried in vacuum, and crushed to obtain the polysaccharide Boutique. The extraction rate is shown in Table 1: it can be seen that the extraction time of 2 hours can meet the requirements.

[0020] Table 1 The influence of extraction time on polysaccharide yield

[0021]

Embodiment 2

[0023] Investigate the effect of solid-liquid ratio on the extraction rate: crush the Herba Sinensis, pass through 60 meshes, place it in an ultrasonic extractor, add different multiples of water, adjust the pH to 8-9, raise the temperature to 90°C, and extract for 2 hours , combined three extracts. After centrifuging for 15 minutes, take the clear liquid; concentrate the extract to a certain volume under reduced pressure, remove the protein by savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, dried in vacuum, and crushed to obtain the polysaccharide Boutique. The extraction rate is shown in Table 2: It can be seen that the solid-liquid ratio is 1:20-30. Considering that the subsequent process steps will be concentrated and the cost will be increased, it is more economical to choose 1:20.

[0024] The influence of table 2 solid-liquid ratio on polysaccharide yield

[0025]

Embodiment 3

[0027] Investigate the effect of extraction temperature on the extraction rate: crush the Herba huixin, pass through 60 meshes, put it in an ultrasonic extractor, add 20 times of water, adjust the pH to 8-9, extract at different temperatures for 2 hours, and combine three extractions liquid. After centrifuging for 15 minutes, take the clear liquid; concentrate the extract to a certain volume under reduced pressure, remove the protein by savage method, then add edible ethanol to make the concentration reach 80%, and the resulting precipitate is refined, dried in vacuum, and crushed to obtain the polysaccharide Boutique. The extraction rate is shown in Table 3: it can be seen that the yield is the highest when the extraction temperature is 90°C.

[0028] Table 3 The influence of extraction temperature on polysaccharide yield

[0029]

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PUM

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Abstract

The invention discloses a method for extracting and purifying polysaccharide of large leaf moss from the large leaf moss. The method comprises the following steps of: crushing the large leaf moss, extracting crude polysaccharide from medicinal materials with water in an ultrasonic extractor, carrying out decentralization, filtering, concentration, protein removing to the extract, using alcohol to precipitate out of water extract, obtaining water-soluble crude polysaccharide of the large leaf moss, further carrying out separation and purification to the crude polysaccharide, vacuum-drying and obtaining the refined product of polysaccharide of the large leaf moss. The method can extract the polysaccharide of the large leaf moss from the large leaf moss to a maximum extent, is safe, non-toxic and reliable, is easy to operate, has high yield and provides possibility for exerting the drug effect of the polysaccharide of the large leaf moss and preparing drugs or health foods for various indications.

Description

technical field [0001] The invention belongs to a method for extracting active components of traditional Chinese medicine, and more specifically relates to a method for extracting and purifying polysaccharides from Herba huixincao. Background technique [0002] At present, nearly a hundred kinds of polysaccharide compounds have been extracted. Through pharmacological research and clinical application, it has been proved that polysaccharides have the functions of anti-tumor, lowering blood fat, anti-thrombosis, enhancing immunity, and delaying aging. Therefore, more and more research and development of polysaccharides being valued by people. Polysaccharides are natural macromolecular compounds that exist in animals, plants and microorganisms. Polysaccharides are generally extracted from natural products. Natural polysaccharides are a class of important biologically active substances with various biological activities and health functions. They can be used as effective drugs a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P9/10
Inventor 张惠芬李宝才戴伟峰何静
Owner KUNMING UNIV OF SCI & TECH
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