Method for detecting 1494 C-T and 1555 A-G mutation of maternally inherited deafness mitochondrial genes, and kit thereof

A 1494C-T, mitochondrial gene technology, applied in the field of genetic engineering, can solve the problems of inability to achieve fast, accurate, and low cost, and achieve the effects of avoiding drug-induced deafness, simplifying the experimental process, and being easy to operate
CN101684498AInactive Publication Date: 2010-03-31董大光
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
董大光
Publication Date
2010-03-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for detecting 1494 C-T and 1555 A-G mutation of maternally inherited deafness mitochondrial genes. The method comprises the steps of designing improved primers and detecting 1494 C-T and 1555A-G mutation through Touch-down PCR amplification and agarose gel electrophoresis. The method has the advantages of simplicity, low cost, direct reliable detection result andapplicability to the large-scale screening or preventive examination for the 1494 C-T and 1555 A-G mutation of maternally inherited deafness mitochondrial genes.
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Description

Technical field:

[0001] The invention relates to the technical field of genetic engineering, in particular to a method for detecting the 1494-position C-T mutation and the 1555-position A-G mutation of the mitochondrial gene of maternally inherited deafness. The present invention also relates to the preparation of a kit for in vitro detection of the 1494 C-T mutation and the 1555 A-G mutation of the mitochondrial gene of maternally inherited deafness according to the method, and the application of the kit in detecting the 1494 C-T mutation and the 1555 A-G mutation . Background technique:

[0002] Aminoglycoside antibiotics (streptomycin, gentamycin, kanamycin, tobramycin and micronomycin, etc.) are widely used clinically because of their broad-spectrum and high-efficiency antibacterial effects and low prices. Controls Gram-negative and positive bacterial infections, but these antibiotics have severe ototoxic effects and can cause irreversible hearing loss in patients. Stu...

Claims

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