Method for producing high protein bean pulp by microbial fermentation

A technology of microbial fermentation and high protein, applied in animal feed, animal feed, application, etc., can solve the problems of affecting the absorption and utilization of soybean protein, low digestibility and biological potency, and ignoring protein, so as to increase animal feed intake , Improve palatability, improve protein quality

Inactive Publication Date: 2012-11-21
DALIAN LONGFENGXIANG ECOLOGICAL AGRI SCI & TECH
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AI Technical Summary

Problems solved by technology

However, previous reports often only focused on the protein content and ignored the protein quality. 80% of the protein in soybeans has a molecular weight of more than 100kd, and there are problems of low digestibility and biological potency, which largely affects the absorption of soybean protein. use

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] 1. Raw materials and processing

[0024] Fresh non-mildew soybean meal, corn and bran are crushed to 40-100 mesh, mixed evenly according to the ratio of soybean meal:bran:corn=7:2:1, and steam sterilized at 121°C for 20-30 minutes.

[0025] 2. Activation of fermentation strains

[0026] Before the strains are inserted into the fermentation, they should be inserted into the activation medium from the preservation slope. The Bacillus subtilis activation medium is beef extract peptone medium, and the yeast activation medium is wort juice. Activate at 37°C and 28°C for 15-30 hours, respectively. In a preferred manner, the activation time of Bacillus subtilis and Saccharomyces cerevisiae are 15-24 hours and 20-30 hours respectively.

[0027] Three, one-step fermentation method

[0028] Inject Bacillus subtilis and Saccharomyces cerevisiae into the mixture of soybean meal, corn and bran at a ratio of 1:1-5, control the total inoculation amount at 1-10%, add 0.4-1.2 times ...

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Abstract

The invention discloses a method for producing high protein bean pulp by microbial fermentation, which takes bean pulp as main raw material as well as corn and wheat bran as auxiliary materials; the materials are ground, evenly mixed together and then inoculated with saccharomyces cerevisiae and bacillus subtilis for solid state fermentation; and after being dried at the temperature of 40-80 DEG C, the product can be taken as animal feed. By adopting multi-strain solid state fermentation and utilizing mixed bacteria synergy, the method not only increases the protein content of the bean pulp, but also degrades macromolecule protein which is difficult to digest in the bean pulp to a certain degree. Simultaneously, the palatability of the fermented the bean pulp can be improved by mellow smell generated in the process of fermentation of yeast, so that the feed intake can be increased.

Description

technical field [0001] The invention relates to a processing method of animal feed, in particular to a method for producing soybean meal feed based on microbial fermentation technology. Background technique [0002] Soybean is an excellent plant protein resource. The processed soybean meal has the characteristics of high digestion and absorption rate, good amino acid composition, reasonable price and abundant resources. It is one of the available plant protein sources in the feed industry. Soybean meal is a by-product of soybean oil extracted by leaching. It is cheap and has a wide range of sources. Its protein content is as high as 43%-48%. Except for the lack of sulfur-containing amino acids, the rest of the amino acid composition is reasonable, especially for other plant feeds Lysine, which is easily lacking, has a content as high as 2%-2.5%. Soybean meal also contains biologically active substances such as isoflavones and phospholipids, as well as other nutrients such as...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/14A23K10/12A23K10/30
Inventor 王际辉刘稳结
Owner DALIAN LONGFENGXIANG ECOLOGICAL AGRI SCI & TECH
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