Method for assembling zinc oxide nano-chain by using DNA as template

A zinc oxide nano and template technology, applied in the direction of zinc oxide/zinc hydroxide, nanotechnology, nanotechnology, etc., can solve the problems of harsh process conditions, expensive synthesis equipment, long reaction time, etc., and achieve simple raw materials and short reaction time , The effect of low product cost

Inactive Publication Date: 2010-06-02
TIANJIN UNIV
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AI-Extracted Technical Summary

Problems solved by technology

For example: the uniform precipitation method is used to prepare nano-zinc oxide, the process conditions are harsh, the equipment requirements are high, and the reaction time is long; the solution-gel method is high in cost and expensive; the microemulsion method is...
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Abstract

The invention discloses a method for assembling a zinc oxide nano-chain by using DNA as a template, comprising the following steps of: (1) mixing and stirring an aqueous solution of DNA, an aqueous solution of zinc nitrate and an aqueous solution of hexamethylenetetramine; (2) heating to 60 DEG C-80 DEG C and keeping the temperature for 0.5-4h; and (3) reducing the temperature to room temperature of 20 DEG C-25 DEG C and carrying out ultrasound treatment in an ultrasound wave dispersion instrument for 10-20 minutes and sampling to obtain the zinc oxide nano-chain by using the DNA as the template. The method is simple, easily obtains raw materials, consumes less energy and reduces the pollution to the environment; the equipment is less and the cost is low, so the method is suitable for batch production; the reaction time is short, the yield is high and no toxicity and harm exist, so that the method provides the possibility for preparing the zinc oxide nano-chain on a large scale; and zinc oxide nano-chains with different shapes can be constructed by operating the space structure of biological macromolecule DNA. The invention can simultaneously solve the problems of the control of the size and the shape of nanometer materials and the dispersion stability.

Application Domain

Technology Topic

Biological macromoleculeBatch production +16

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  • Method for assembling zinc oxide nano-chain by using DNA as template
  • Method for assembling zinc oxide nano-chain by using DNA as template

Examples

  • Experimental program(4)

Example Embodiment

Example 1
25ml DNA aqueous solution with a concentration of 0.336mg/ml, 25ml zinc nitrate aqueous solution with a concentration of 4.88mM and 25mL hexamethylenetetramine (HMT) aqueous solution with a concentration of 4.88mM were mixed in an Erlenmeyer flask and stirred for 10min;
Then the water bath is heated to 70°C, and the temperature is controlled to be kept at 70°C for 3 hours;
The temperature is lowered to room temperature, and the sample is prepared by ultrasonic treatment in an ultrasonic disperser for 15 minutes to obtain a zinc oxide nanostrand assembled with DNA as a template. See Figure 2.

Example Embodiment

Example 2
(1) Mix the DNA aqueous solution with a concentration of 0.1mg/ml, 6mM zinc nitrate aqueous solution and 5mM hexamethylenetetramine aqueous solution in a volume ratio of 1:0.5:1, and stir for 30min;
(2) Heat to 60℃, keep for 4 hours;
(3) Cool down to room temperature 20°C, sonicate the sample in an ultrasonic disperser for 10 minutes, and obtain a DNA as a template to assemble zinc oxide nano-strands.

Example Embodiment

Example 3
(1) Mix the DNA aqueous solution with a concentration of 0.50mg/ml, 4mM zinc nitrate aqueous solution and 1mM hexamethylenetetramine aqueous solution in a volume ratio of 1:1:2, and stir for 5min;
(2) Heat to 70℃, keep for 2h;
(3) Cool down to room temperature 22°C, sonicate the sample in an ultrasonic disperser for 15 minutes to prepare a sample using DNA as a template to assemble zinc oxide nano-strands.
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