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Method for preventing cell-carrying sodium alginate microcapsule from shrinking

A technology of sodium alginate and calcium alginate, applied in microcapsules, capsule delivery, pharmaceutical formulations, etc., can solve the problems of affecting the molecular interception performance of microcapsule composite membranes, affecting biocompatibility, and shrinking microcapsules.

Inactive Publication Date: 2010-06-09
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] High-voltage electrostatic droplet method and vibrating nozzle method have been widely used in the preparation of APA microcapsules, and the preparation process of microcapsules is becoming more and more mature. Chitosan complexation reaction, after the composite film is formed, the shrinkage and deformation of the microcapsules are easy to occur, making the obtained microcapsules irregular in shape and rough in surface, thus affecting the molecular retention performance of the microcapsule composite film, and also Can affect biocompatibility in vivo

Method used

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  • Method for preventing cell-carrying sodium alginate microcapsule from shrinking
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  • Method for preventing cell-carrying sodium alginate microcapsule from shrinking

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preparation example Construction

[0026] The preparation process of sodium alginate microcapsules of the above-mentioned cells is often reported in the literature (Yang Xiaoming, Bi Haosheng, Yang Maoyuan, etc., research on the in vitro culture of microencapsulated human chromaffin cells [J]. Chinese Journal of Anesthesiology, 2001; 21 (7) : 410-413. Xue Yilong (CN1345234, microencapsulated bovine adrenal medulla chromaffin cell drug for treating pain), does not belong to the content of the present invention.

[0027] The present invention provides a new method for preventing the shrinkage of sodium alginate microcapsules, that is, after cells are encapsulated in sodium alginate solution to form calcium alginate microspheres (after the second step), that is, after coating polylysine or before chitosan (before step 3), place the calcium alginate microspheres in the polyol solution for processing, let it stand, remove the lower layer of calcium alginate microspheres to precipitate, and further react with polylysi...

Embodiment 1

[0028] Example 1: Preparation of APA microcapsules coated with bovine adrenal medulla chromaffin cells BCC

[0029] (1) The total number of cells is 1×10 61 bovine adrenal medulla chromaffin cells (BCC) were mixed with 1 ml of 1.5% sodium alginate solution, and made into a suspension by stirring.

[0030] (2) Use a high-voltage electrostatic droplet generator to spray the suspension into 50ml of calcium chloride solution with a concentration of 100mmol / L, and after standing for 10min, obtain calcium alginate with a diameter in the range of 180-500μm The microspheres were precipitated, and the supernatant was removed after the precipitation was complete.

[0031] (3) Then add the calcium alginate microspheres into 50ml of mannitol solution with a concentration of 0.3mol / L, place it at room temperature for 5-10min, and remove the supernatant after the precipitation is complete.

[0032] (4) Then put the precipitate in 0.05% polylysine solution, mix well, place at room temperat...

Embodiment 2

[0036] Example 2: Preparation of APA microcapsules coated with rat pancreatic islet tissue. The steps are the same as in Example 1, but the difference from Example 1 is that the polyol solution used in step (3) is in a physiological isotonic sorbitol solution, placed at room temperature for 20 min, and the supernatant is removed after the precipitation is complete.

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Abstract

The invention discloses a method for preventing a sscell-carrying sodium alginate microcapsule from shrinking, which is a method for preparing the sodium alginate microcapsule wrapping cells by using sodium alginate-polylysine or chitosan-sodium alginate as compound wrapping materials. The method is characterized in that a polyol solution is adopted to treat calcium alginate microballoons in the preparing process of the sodium alginate microcapsule, thereby preventing the prepared sodium alginate microcapsule from shrinking.

Description

technical field [0001] The invention relates to the field of biomedicine of microencapsulated cell engineering, and more specifically relates to a new method for preventing the shrinkage of cell-laden sodium alginate microcapsules. Background technique [0002] Microcapsules are used to encapsulate tissue cells with specific functions to form immune-isolated artificial cells, which can be implanted into diseased animals or patients to supplement the lack of physiologically active substances in the diseased body, so as to achieve the purpose of treating diseases. [0003] The three-layer structure membrane microcapsule made of sodium alginate-polylysine-sodium alginate (APA) or sodium alginate-chitosan-sodium alginate (ACA) has a small size (particle size 200-1000μm) ), good biocompatibility and large surface area, which is conducive to the long-term survival of microencapsulated cells in animals. A large number of experiments have shown that the capsule of APA microcapsules...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K47/34A61K35/00A61K47/36
Inventor 刘善奎景颖张爱兵龚非
Owner UNIV OF JINAN
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