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Method for culturing coriolus versicolor induced oxalate decarboxylase by using straw carbon source

A technology for oxalate decarboxylase and Coriolus versicolor, which is applied in the field of preparation of oxalate decarboxylase, can solve the problems of air pollution, increased greenhouse effect and the like, and achieves the effects of easy operation, simple method and high conversion rate

Inactive Publication Date: 2010-06-09
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the burned straw can be spread on the fields as fertilizer, the gas produced during the burning process will increase the greenhouse effect, and the smoke generated will also cause air pollution

Method used

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  • Method for culturing coriolus versicolor induced oxalate decarboxylase by using straw carbon source
  • Method for culturing coriolus versicolor induced oxalate decarboxylase by using straw carbon source
  • Method for culturing coriolus versicolor induced oxalate decarboxylase by using straw carbon source

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Production of Coriolus versicolor from wheat straw hydrolyzate (the initial pH value of the medium is 5.0)

[0033] Weigh the fully ground wheat straw, add a sulfuric acid solution with a concentration of 1% at a solid-to-liquid ratio of 1g: 12ml, soak the wheat straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure steam sterilizer, and heat it at 121 At ℃, react for 30 minutes, filter and remove the residue of wheat straw, obtain the hydrolyzate of wheat straw for later use, measure the content of reducing sugar (using glucose as the standard sugar) in the hydrolyzate of wheat straw by DNS method, and store it at 4°C for later use;

[0034] Add wheat straw hydrolyzate containing 1g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O ...

Embodiment 2

[0037] Production of Coriolus versicolor from wheat straw hydrolyzate (the initial pH value of the medium is 5.0)

[0038] Weigh the fully ground wheat straw, add a sulfuric acid solution with a concentration of 3% at a solid-to-liquid ratio of 1g: 15ml, soak the wheat straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 100°C , reacted for 60 minutes, filtered to remove the straw residue, and obtained the wheat straw hydrolyzate for later use. The content of reducing sugar in the wheat straw hydrolyzate (using glucose as the standard sugar) was determined by the DNS method, and stored at 4°C for later use;

[0039] Add wheat straw hydrolyzate containing 1.5g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, ...

Embodiment 3

[0042] Production of Coriolus versicolor from wheat straw hydrolyzate (the initial pH value of the medium is 5.0)

[0043] Weigh the fully ground wheat straw, add a sulfuric acid solution with a concentration of 8% at a solid-to-liquid ratio of 1g:5ml, soak the wheat straw powder in a dilute sulfuric acid solution for 10 hours, place it in a high-pressure cooker, and heat it at 150°C , reacted for 20 minutes, filtered to remove the straw residue, and obtained the straw hydrolyzate for later use. The content of reducing sugar in the wheat straw hydrolyzate was determined by DNS method (using glucose as the standard sugar), and it was stored at 4°C for later use;

[0044] Add wheat straw hydrolyzate containing 0.35g reducing sugar (calculated as glucose) to every 50mL medium, then add 0.15g peptone, 0.05g potassium dihydrogen phosphate, 0.025g MgSO 4 ·7H 2 O, 0.01g Na 2 HPO 4 12H 2 O, and 50 μL of trace elements (the formula of trace elements is: FeSO 4 ·7H 2 O 10g / L, MnS...

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Abstract

The invention relates to a method for culturing coriolus versicolor induced oxalate decarboxylase by using a straw carbon source, which comprises the following steps of: (1) soaking crushed straws in acid solution, and hydrolyzing the crushed straws at the temperature of between 100 and 150 DEG C; (2) preparing a fermentation medium, and adjusting the pH value to between 4.0 and 5.0; (3) taking a mycelium, inoculating the mycelium into a liquid seed culture medium, when a white mycelium group is formed but is not emerged on the water surface, transferring the mycelium and the culture medium to a container containing sterile glass beads, vibrating the container to break the mixture, and inoculating the mycelium into the fermentation medium according to the inoculum size of 3 to 15 percent; and (4), after 4 to 6 days of culture, adding oxalic acid into the container, performing the culture for 0.5 to 6 days to obtain the mycelium, grinding and crushing the mycelium after the mycelium is chilled by liquid nitrogen, extracting mycelial fragments by using an acetate buffer, and centrifuging the mycelial fragments. The method is simple, is easy in operation and is low in cost, the preparation of the carbon source has strong controllability, and the sugar content is stable.

Description

technical field [0001] The invention belongs to the field of preparation of oxalate decarboxylase, in particular to a method for cultivating Coriolus versicolor as a carbon source of wheat straw to induce oxalate decarboxylase. Background technique [0002] Oxalate decarboxylase can catalyze the decarboxylation of oxalate to form formic acid and carbon dioxide, so it has broad application prospects in the enzymatic detection of oxalate content, the degradation of oxalate in industrial wastewater, the preparation of low-oxalate-content foods, and the reduction of oxalate concentration in the human body. Coriolus versicolor is an important strain for producing oxalate decarboxylase, how to reduce its culture cost, produce mycelium in large quantities, and induce mycelium to synthesize oxalate decarboxylase is an important issue. Since carbon is the most abundant element in organisms, the carbon source is the most used in the medium and accounts for a relatively high proportion...

Claims

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Application Information

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IPC IPC(8): C12N9/88
Inventor 洪枫陈奇知曹张军杨光杨雪霞
Owner DONGHUA UNIV
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