Vertical current western blotting method for quickly detecting human immunodeficiency virus-I antibody in urine

A technology of human immunodeficiency and immunoblotting, which can be used in measurement devices, instruments, scientific instruments, etc., can solve the problems of inability to be used as a routine detection method, low concentration of HIV-1 antibody, and long binding time, and achieves great popularization and application value. , shorten the detection time, improve the effect of detection specificity

Inactive Publication Date: 2010-06-09
ZHEJIANG ACAD OF MEDICAL SCI
View PDF0 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, studies have shown that the concentration of HIV-1 antibodies in urine is low, especially the high concentration of p24 antibodies in serum is low, so urine detection technology needs to be improved
[0004] Detecting HIV-1 antibodies in urine samples b...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vertical current western blotting method for quickly detecting human immunodeficiency virus-I antibody in urine
  • Vertical current western blotting method for quickly detecting human immunodeficiency virus-I antibody in urine
  • Vertical current western blotting method for quickly detecting human immunodeficiency virus-I antibody in urine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0039] 1. Material preparation

[0040] 1. Reagents:

[0041] (1) Lysis solution: 1M tris-hydrochloric acid (pH8.8), 7% (W / V) sodium lauryl sulfate;

[0042](2) Electrophoresis solution: 10g of glycine, 6g of trishydroxymethylaminomethane, 2g of sodium lauryl sulfate dissolved in 1 liter of distilled water (PH8.7);

[0043] (3) Electrotransfer solution: 13g of sodium chloride, 2g of disodium edetate, and 9.5g of trishydroxymethylaminomethane were dissolved in 1 liter of distilled water (PH7.5);

[0044] (4) blocking solution: 5% bovine serum albumin;

[0045] (5) Adhesive: α-ethyl cyanoacrylate instant adhesive;

[0046] (6) 10 times rinse solution: Sodium chloride 80g, disodium hydrogen phosphate 28.8g, potassium dihydrogen phosphate 2.4g dissolved in 1 liter of distilled water (PH7.0);

[0047] (7) Conjugate: alkaline phosphatase-labeled rabbit anti-human IgG, diluted 1:5000;

[0048] (8) Substrate: 1.2 g of 5-bromo-4-chloro-3-indolyl phosphate and 2.3 g of nitroblue te...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an analysis method of immunochemistry and biochemistry, and discloses a vertical current western blotting method for quickly detecting a human immunodeficiency virus-I (HIV-1) antibody in urine, which is particularly suitable for infants and people who are difficult in collecting blood and are unwilling to collect blood. The vertical current western blotting method comprises the following steps: coating HIV-1 antigen to an NC membrane through electrophoresis and electric conversion of lauryl sodium sulfate and polyacrylamide gel, then sticking the film to the bottom of a self-made reaction tank, and combining the reaction tank with a vacuum filtration system. The method enables a urine sample to vertically pass through the NC membrane in a controllable time so as to accelerate the antigen antibody reaction, increase the combination degree of the antigen and the antibody and further improve the detection sensitivity. The method can quickly detect the HIV-1 antibody in the urine, and ensure the HIV-1 infection condition. The method can be used for laboratory diagnosis and confirmation, medicament treatment effect evaluation and the like, is suitable for lower popularization and use, and has large popularization and application values.

Description

technical field [0001] The present invention provides an immunochemical and biochemical analysis method—vertical flow immunoblotting method, which is especially suitable for rapid detection of human immunodeficiency virus-type 1 (HIV-1) antibody in urine by infants, those who have difficulty in taking blood and those who are unwilling to take blood vertical flow immunoblotting. Background technique [0002] Since it was discovered in the 1980s, no effective means of prevention and cure have been found for human immunodeficiency virus (HIV) infection. Early diagnosis and early isolation are still the main ways to prevent its further spread. The methods used to detect HIV infection include nucleic acid detection, antibody detection, and viral load detection, among which antibody detection is prescribed by the World Health Organization and the Ministry of Health as the main method of clinical detection because of its simplicity and speed. Western blotting (WB) is an important ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/574G01N33/561
Inventor 倪崖谭卓黄云坚李坤房国坚李忠辉
Owner ZHEJIANG ACAD OF MEDICAL SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap