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Extract of effective part of sibiraea augustata(rehd.) hand-mazz for preventing adiposity and lowering hyperlipoidemia, preparation method and application thereof

A technology of effective parts and extracts, applied in the field of extracts of effective parts of willow tea and preparation thereof

Active Publication Date: 2010-06-16
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are "Effect of Willow Tea Water Extract on the Growth of K562 Tumor Cells", "Experimental Research on the Immunomodulation Effect of Willow Tea Extract", "Experimental Observation of the Effect of Willow Tea on the Antioxidative Ability and Immune Organs of Mice", "Willow Tea Extract The protective effect and mechanism of green tea on CCl4 liver damage" and "Research on lipid metabolism regulation of willow tea" have been reported, but "prevention of obesity and treatment of hypercholesterolemia (lowering blood triglyceride ( TG), low-density lipoprotein-cholesterol (LDL-C) and lipoprotein (a) concentration, improve blood viscosity) the effective part extract of willow tea "report

Method used

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  • Extract of effective part of sibiraea augustata(rehd.) hand-mazz for preventing adiposity and lowering hyperlipoidemia, preparation method and application thereof
  • Extract of effective part of sibiraea augustata(rehd.) hand-mazz for preventing adiposity and lowering hyperlipoidemia, preparation method and application thereof
  • Extract of effective part of sibiraea augustata(rehd.) hand-mazz for preventing adiposity and lowering hyperlipoidemia, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, preparation effective part extract of willow tea

[0038]Dried or fresh willow tea (Sibiraea angustata (Rehd.) Hand.-Mazz.) or Xianbei flower (Sibiraea laevigata (L.) Maxim.) is used as raw material, washed and crushed, and cooled with water respectively Soaking and thermal extraction are two methods of extraction, and the ratio of water to raw materials is 8:1-15:1 respectively.

[0039] 1. Cold leaching: extract the pulverized raw materials with water at room temperature for 12-24 hours, filter, and filter the residue again at room temperature for 6-12 hours, filter off the residue, combine the two filtrates, and concentrate the filtrate Then get the extract.

[0040] 2. Thermal extraction: heat the raw material with water to reflux for half an hour to 1 hour, then filter, and filter the residue again with water to reflux for half an hour, combine the two filtrates, and concentrate the filtrate to obtain an extract. The extraction rate of the total ext...

Embodiment 2

[0042] Example 2, willow tea ethyl acetate extract inhibits the proliferation and differentiation of preadipocytes

[0043] 1) The proliferation of preadipocytes was detected by MTT method: the 3T3-L1 preadipocytes were treated with 100 mg / mL and 200 mg / mL ethyl acetate extracts of willow tea prepared in Example 1 respectively. The MTT results showed that the ethyl acetate extracts of willow tea Drugs have obvious inhibitory effect on the proliferation of preadipocytes, and have obvious time- and dose-dependent effects, as shown in Figure 1.

[0044] 2) Cell cycle detection by flow cytometry: 3T3-L1 preadipocytes were treated with 100 mg / mL and 200 mg / mL ethyl acetate extracts of willow tea prepared in Example 1, respectively, and cell cycle detection was carried out respectively. The results showed that: willow tea Ethyl acetate extract can prevent cells from entering the S phase, thereby inhibiting the increase in the number of preadipocytes, such as figure 2 shown.

[004...

Embodiment 3

[0047] Embodiment 3, the influence of ethyl acetate extract of willow tea on mice in different growth stages

[0048] A. Effects on growing mice:

[0049] Experimental method: 50 4-week-old Kunming mice, weighing 12-15 g, were randomly divided into 5 groups (blank control, positive drug control group and 3 experimental drug dosage groups) with 10 mice in each group. Except basal feed, 3 experimental groups give the diluent of the willow tea ethyl acetate extract of different doses by intragastric administration every day, and dosage is converted into 80%, 100% and 120% of dosage (g / w) of human (50Kg) every day Administration; the positive drug control group was given Kangershou capsule powder solution by intragastric administration every day, and the dosage calculation was the same as above; the blank control group was given the same volume of distilled water as the experimental group every day. Raise for 8 days and weigh once a day.

[0050] The experimental results show th...

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Abstract

The invention relates to the field of traditional Chinese medicines, in particular to an extract of the effective part of sibiraea augustata(rehd.) hand-mazz for preventing adiposity and lowering hypercholesteremia, a preparation method and an application thereof. According to the prevention of the adiposity in the invention, the extract of the effective part of the sibiraea augustata(rehd.) hand-mazz is obtained by the method comprising the following steps: (1) washing and pulverizing; (2) leaching by water to obtain a total water leaching extract; (3) dissolving the total extract by methanol or ethanol; (4) extracting by chloroform or methylene chloride; and (5) extracting a water solution after being degreased by the chloroform or the methylene chloride by ethyl acetate, decompressing and concentrating to obtain the effective part. The invention provides the effective part of an ethyl acetate extract in the extract of the sibiraea augustata(rehd.) hand-mazz, and the ethyl acetate extract can prevent the adiposity and treat the hypercholesteremia, can suppress the multiplication and the differentiation of precursor fat cells and acts as the function of preventing obesity.

Description

technical field [0001] The present invention relates to the field of traditional Chinese medicine, specifically, the present invention relates to a method for preventing obesity and treating hypercholesterolemia (lowering blood triglyceride (TG), low-density lipoprotein-cholesterol (LDL-C) and lipoprotein ( a) Concentration, improving blood viscosity) the effective part extract of willow tea and its preparation method and application. Background technique [0002] With the development of social economy and the improvement of people's living standards, the nutritional status of human beings has been greatly improved. Obesity and hypercholesterolemia have become key issues affecting human health. Finding effective prevention and / or treatment of obesity and hypercholesterolemia The active ingredients of traditional Chinese medicines have become the focus of attention in this field. [0003] Obesity refers to long-term energy intake exceeding consumption, resulting in excess en...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P3/04A61P3/06A61K127/00A61K135/00
Inventor 何兰翟永功潘宣唐志刚
Owner BEIJING NORMAL UNIVERSITY
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