Cellulose hydrolytic enzyme beta-1,4 glucose incision enzyme gene

An endoglucanase and cellulose technology, applied in genetic engineering, plant genetic improvement, enzymes, etc., can solve problems such as environmental pollution, loss of organic matter components, and damage to soil physical and chemical properties

Inactive Publication Date: 2010-06-16
NANJING AGRICULTURAL UNIVERSITY
View PDF0 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to reports, there are as many as 500 million tons of cellulose-rich materials such as crop straws and rice stalks in my country every year, and the annual plant matter produced by photosynthesis in the world is as high as 1.55×10 9 Tons, 89% of which have not been utilized by peop

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cellulose hydrolytic enzyme beta-1,4 glucose incision enzyme gene
  • Cellulose hydrolytic enzyme beta-1,4 glucose incision enzyme gene
  • Cellulose hydrolytic enzyme beta-1,4 glucose incision enzyme gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Cloning of β-1,4 endoglucanase gene

[0022] 1.1 Construction of functional genome BAC library of red soil microorganisms

[0023] Use 0.2% sodium pyrophosphate aqueous solution as a dispersant to suspend soil particles with a water-to-soil ratio of 3:1, and homogenate as a dispersion method (Joyoung JYL-350, 22,000rpm, 3s, 12 times) to release the bacterial cells from the soil particles, After preliminary separation of bacteria and soil particles by sucrose density gradient centrifugation, the microorganisms (most bacteria and some fungi and actinomycetes spores) were purified by density gradient centrifugation using Nycodenz (Axis-Shield, Norway) as the medium to obtain The bacterial cells were embedded and lysed with low-melting point agarose (BBI) to obtain large fragments of DNA over 400kb in size, partially digested with restriction endonuclease Sau3AI, and then separated by alternating pulse field electrophoresis (BIO-RAD) and electroelution to collect DNA fr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a gene of cellulose hydrolytic enzyme beta-1,4 glucose incision enzyme, which belongs to the field of application industrial microorganisms. The invention provides the gene of one of key enzymes in a cellulose hydrolytic enzyme system, namely the gene of the beta-1,4 glucose incision enzyme and a derived hydrolytic enzyme protein thereof. The gene has the full length of 1,332 bp, the G+C content of 43.77 percent and 443 encoded amino acids, and is a new gene resource for the cellulose hydrolytic enzyme. An engineering strain constructed by using the gene can efficiently express the beta-1,4 glucose incision enzyme, and the Km value and the Vmax of the enzyme are 19.92 mg/ml and 1,941 mumolmin-1mg-1 respectively when sodium carboxymethylcellulose is used as a substrate. A produced enzyme preparation can be used in the industries of foods, medicaments, feedstuffs, textiles, washing and the like, and not only can solve the recycling problem of cellulose but also can obtain considerable economic benefits.

Description

1. Technical field [0001] The invention relates to a cellulolytic enzyme β-1,4 endoglucanase gene, which belongs to the field of applied industrial microorganisms. The enzyme preparation produced is used in industries such as food, medicine, feed, textiles, and washing, and can not only solve the problem of fiber It can also achieve considerable economic benefits. 2. Background technology [0002] Cellulose is the main component of the cell wall of higher plants, accounting for 30% to 50% of the total dry weight of plants. It is the most widely distributed and abundant renewable carbon source compound on the earth, accounting for 40% of the total biomass of the earth. According to reports, there are as many as 500 million tons of cellulose-rich materials such as crop straws and rice stalks in my country every year, and the annual plant matter produced by photosynthesis in the world is as high as 1.55×10 9 Tons, 89% of which have not been utilized by people, and the utilizat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N15/56C12N9/42C12N15/70C12N1/21C12R1/19
Inventor 崔中利刘娟曹慧刘卫东
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap