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Micro-satellite marker positioning of dwarf gene of germplasm line Shannong 495

A technology of microsatellite markers and dwarf genes, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., and can solve problems such as accuracy impact, rapid development, and loose linkage

Inactive Publication Date: 2010-06-16
朱玉丽
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the dwarf gene molecular markers have a large genetic distance with the genes, and the linkage is not tight, which affects the accuracy of selection.
Although microsatellite research on wheat started relatively late, it has developed rapidly

Method used

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  • Micro-satellite marker positioning of dwarf gene of germplasm line Shannong 495
  • Micro-satellite marker positioning of dwarf gene of germplasm line Shannong 495
  • Micro-satellite marker positioning of dwarf gene of germplasm line Shannong 495

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Experimental program
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Effect test

Embodiment Construction

[0046] 1.1 Materials

[0047] 1.1.1 Wheat material

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Abstract

The invention aims to select and breed a dwarf germplasm line Shannong 495 from filial generations of an amphidiploid Am6 (AABBDD), which is synthesized from a diploid aegilops tauschii (DD) and a tetraploid durum wheat (AABB), and common wheat Ji'nan 17, and perform SSR molecular marker positioning on a dwarf gene of the dwarf germplasm line Shannong 495 in the hope of laying a foundation for further researches and marker-assisted selections of the dwarf gene.

Description

technical field [0001] The microsatellite marker mapping of the dwarf gene of the germplasm line Shannong 495 belongs to the important field of molecular biology and wheat germplasm resources research. Background technique [0002] In the 1960s, crops were lodging due to heavy fertilization, which greatly reduced crop yields around the world. Until the emergence of some dwarf and semi-dwarf varieties resistant to lodging, the crop yield has been greatly improved, which has caused a "green revolution" that has attracted worldwide attention (Journal of Shandong Agricultural University (Natural Science Edition), 2006, 37( 3): 359~362). In wheat, Rht-B1b and Rht-D1b from Norin 10 and Rht8 and Rht9 dwarf genes from Japanese red wheat have been widely used in wheat varieties all over the world. Dwarf breeding has attracted great attention from breeders and has become a major approach in high-yield breeding [2] . [0003] Among the molecular markers of wheat dwarf genes, RFLP m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 朱玉丽
Owner 朱玉丽
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