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Mulberry antergic endophyte Bacillus tequilensis

An endophyte Bacillus tequila, antagonistic technology, applied in the direction of bacteria, microorganisms, fungicides, etc., can solve the problems of pesticide residues, environmental pollution, and difficult to guarantee the safety of mulberry food, etc., to achieve strong thermal stability, strong antagonistic effect

Active Publication Date: 2016-01-27
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of chemical pesticides will not only bring negative impacts such as environmental pollution and pesticide residues, but also make it difficult to guarantee the food safety of mulberries.

Method used

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  • Mulberry antergic endophyte Bacillus tequilensis
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  • Mulberry antergic endophyte Bacillus tequilensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Isolation, purification and screening of endophytic antagonistic bacteria in mulberry trees

[0026] 1. Isolation and purification of mulberry endophytes

[0027] 1.1. Isolation of endophytes:

[0028] Collection and storage of samples: Mulberry stems were collected from mulberry fields, washed with tap water, dried, numbered and stored at 4°C, and endophytic bacteria were isolated in time.

[0029] Isolation of endophytes: cut the stems of mulberry trees into small pieces (about 5.0cm in length), soak them in 75.0% alcohol completely, take them out, ignite the alcohol on the surface of the stems on an alcohol lamp, and wait for the alcohol to burn out. Roll the mulberry branches sterilized on this surface on the blank medium, so that each surface of the mulberry branches is in contact with the medium, and use it as a blank control to verify whether the surface disinfection is thorough. Then it was placed in a sterile petri dish and cut open in three la...

Embodiment 2

[0037] Embodiment 2: The biological characteristic of 7PJ-16 bacterial strain

[0038] 1. The effect of temperature on the growth of antagonistic bacteria 7PJ-16

[0039] Inoculate the antagonistic bacteria 7PJ-16 strain into PDB liquid medium (1% inoculum volume, 25% bottle volume), shake at 180r / min at 5°C, 15°C, 25°C, 35°C, 45°C and 55°C respectively Cultivate, take the bacterial solution regularly to measure its OD 600 The value (PDB medium was adjusted to zero), the same treatment was repeated 3 times to get the average value. Taking culture time as abscissa, OD 600 Make a growth curve for the ordinate, and calculate the generation time of the endophytic antagonistic bacteria 7PJ-16 strain at different temperatures.

[0040] Effect of Fermentation Time on Antibacterial Active Substances Produced by Antagonistic Bacteria 7PJ-16 Strain

[0041] The antagonistic bacterium 7PJ-16 strain was inoculated in PDB medium (1% inoculum volume, 30% bottle volume), and cultur...

Embodiment 3

[0042] Embodiment 3: thermal stability and growth promoting effect of 7PJ-16 fermented liquid

[0043] 1. Detection of thermal stability of active fermentation liquid of antagonistic bacteria 7PJ-16 strain

[0044] The aseptic fermentation filtrate of the antagonistic bacterium 7PJ-16 was placed at 40°C, 60°C, 80°C, and 100°C for 30 minutes, and after cooling to room temperature, the mycelium growth rate method was used to detect its effect on the strain of G. nucleatum SXSG-5. Bacteriostatic activity. Sterilized water was used as the blank control, and aseptic fermentation filtrate without heat treatment was used as the positive control. Each group was repeated 3 times to determine its effect on the growth of pathogenic bacteria.

[0045] Growth-promoting effect of antagonistic bacteria 7PJ-16 on tissue cultured seedlings of mulberry

[0046] The tissue-cultured seedlings with the same growth were taken, and the 7PJ-16 strain bacteria and sterile fermentation liquid w...

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Abstract

A strain of mulberry antergic endophyte Bacillus tequilensis ((i) Bacillus tequilensis( / i)) 7pj-16 (CCTCC No. M2015319) is separated and purified from stems of 'guosangchuan 7637'. The bacteria have a strong and stable antagonistic action to Scleromitrula shiraiana. The bacteria grows in a temperature ranging from 25 DEG C to 45 DEG C, the optimum temperature being 25DEG C; the bacteria has optimum fermentation time of 72 hours; a aseptic active fermentation filtering liquid is heat-stable; the bacteria suppressing effect of aseptic fermentation filtering liquid does not change obviously after treating the fermentation filtering liquid in a temperature of 100DEG C for 30 minutes. The bacterial body and the fermentation liquid have no lethal effect on the mulberry tissue culture plants but has growth-promoting actions, and have potential commercial development and application values.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and in particular relates to a mulberry tree antagonistic endophyte Bacillus tequila ( Bacillus equilensis ). Background technique [0002] Sclerotinia sclerotiorum (Sclerotedisease of mulberryfruit) is commonly called as ginkgo disease, and infected mulberries are often off-white and have a foul smell, thus losing their commodity and edible value. The onset of the disease is fierce and fast, which seriously restricts the healthy development of the fruit and mulberry industry. It occurs in Jiangsu, Zhejiang, Shanghai, Chongqing, Sichuan and other places. The incidence rate is as high as 30% to 90%. The garden is extinct. According to the difference of pathogens and diseases, sclerotinia mulberry is divided into sclerotinia mulberry, hypertrophic sclerotinia mulberry and small grain sclerotinia mulberry. Among them, the pathogenic sclerotinia of mulberry shrinkage S...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01P3/00C12R1/07
Inventor 谢洁任慧爽周泽扬
Owner SOUTHWEST UNIV
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