Tagman probe pair and primer pair for detection of Alzheimer's disease-related loci

A technology of senile dementia and primer pairs, which is applied in the direction of microbiological determination/testing, biochemical equipment and methods, and can solve problems such as lost opportunity for treatment and neglect by family members of patients

Inactive Publication Date: 2012-02-01
SHANGHAI GENECORE BIOTECHNOLOGIES
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  • Description
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Problems solved by technology

However, due to the atypicality of the early symptoms of the disease, it is easy to be ignored by the family members of the patient, thus losing the best opportunity for treatment

Method used

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  • Tagman probe pair and primer pair for detection of Alzheimer's disease-related loci
  • Tagman probe pair and primer pair for detection of Alzheimer's disease-related loci
  • Tagman probe pair and primer pair for detection of Alzheimer's disease-related loci

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Embodiment Construction

[0019] In order to better understand the content of the present invention, the following will be further described in conjunction with specific examples.

[0020] 1. Genomic DNA extraction:

[0021] 1.1 Reagents and instruments:

[0022] 1.1.1 Reagents and consumables: Genomic DNA extraction kit (TIANamp BLOOD DNA kit, DP318-03; TIANamp Micro micro sample genomic DNA extraction kit, DP316; TIANamp buccal swab genomic DNA extraction kit, DP322-03) contains : Cell Lysis Solution CL; Buffer GA; Buffer GS; Buffer GB; Buffer GD; Washing Solution PW; Elution Buffer TB; Carrier RNA; RNase-free ddH2O; Proteinase K (20mg / ml); ; Collection tube (2ML); 1.5ML sterile collection tube; Absolute ethanol.

[0023] 1.1.2 Instruments: DENVILLE260 centrifuge, XW-80A vortex mixer, H.H.S pointer electric heating constant temperature water bath.

[0024] 1.2 Extraction steps

[0025] Genomic DNA Extraction from Whole Blood

[0026] 1.2.1 Take 200 μl of anticoagulated whole blood into a 1.5ml E...

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Abstract

The present invention relates to a detection method for senile dementia-related sites, comprising the steps of: extracting genomic DNA from a human sample; designing a pair of Taqman probe pairs and a pair of primer pairs respectively according to each of at least two senile dementia-related genes, The 5' end and the 3' end of the Taqman probe pair are respectively labeled with a fluorescent reporter group and a fluorescent quencher group, and the genomic DNA is respectively amplified by fluorescent quantitative PCR; according to the results of fluorescent quantitative PCR amplification, the aged Whether the dementia-related genes are mutated, preferably, the number of the senile dementia-related genes is 5, which are APOE1 gene, APOE2 gene, CYP46 gene, CTSD gene and IL1A gene. It is accurate and reliable, and provides a reference for whether to further adopt targeted health management and follow-up detection methods.

Description

technical field [0001] The present invention relates to the technical field of detection of disease-related sites, more specifically, to the technical field of detection methods for senile dementia-related sites, in particular to a detection method for senile dementia-related sites. Background technique [0002] Alzheimer's disease (Alzheimer, AD) is a chronic brain degenerative disease. The clinical manifestations are progressive far and near memory impairment, decline in analysis and judgment, emotional changes, behavioral disturbances, and even confusion, and finally died of pneumonia or urinary tract infection. According to statistics, there are 17-25 million Alzheimer's patients in the world. It is estimated that there are about 6.45 million patients in our country. Moreover, with every 5 years of age increase, the prevalence of AD will increase twice, and 20% of the patients aged 80-85 suffer from senile dementia. It is the fourth cause of death after heart disease,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈奕雄赵翊均
Owner SHANGHAI GENECORE BIOTECHNOLOGIES
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