Six pairs of primers for the detection of breast cancer related loci

Abstract

The invention relates to a method for detecting breast cancer-related sites, comprising the steps of: extracting genomic DNA from a human sample; designing at least two primer pairs according to the gene sequences of Brca1 and Brac2, performing PCR amplification on the genomic DNA, and recovering the amplified product; and then Sequencing, and analyzing the sequencing results to determine whether a mutation occurs, preferably, the primer pair detects one or more of the 321st, 337th, 346th, 891st and 1458th codons of the Brca1 gene, and one or more of the 814th, 936th to 937th, 949th, 1037th and 2156th to 2157th codons of the Brca2 gene. Whether to further adopt targeted health management and follow-up detection methods to provide a reference.

Description

technical field [0001] The present invention relates to the technical field of detection of disease-related sites, more specifically, to the technical field of detection methods for breast cancer-related sites, in particular to a detection method for breast cancer-related sites. Background technique [0002] Breast cancer is a malignant tumor that occurs in the glandular epithelial tissue of the breast. It is the most common malignant tumor in women. About 1.2 million women in the world suffer from breast cancer every year, and 500,000 people die from breast cancer. The incidence of breast cancer in China is increasing year by year. At present, it has reached 5 out of 10,000. Studies have shown that breast cancer is one of the cancers with the most significant genetic predisposition, and 5% to 10% of breast cancers come from family inheritance. Breast cancer susceptibility genes BRCA1, BRCA2 mutations are highly related to breast cancer and have dominant genetic characteris...

AI Technical Summary

Problems solved by technology

However, traditional breast cancer detection methods mainly include: X-ray diagnosis, ultrasonography, cytology and histological diagnosis, etc. These methods play an important role in the routine detection of breast cancer, but they are generally not suitable for real breast cancer detection. Accurate early diagnosis of breast cancer. With the progress of molecular biology, cell biology and immunology research, breast cancer research has entered the field of molecular pathology from cytopathology, and biodiagnostic technology has become a promising treatment. In particular, the fruitful results obtained in human genome research in recent years have further promoted the development of biodiagnostic technology, and molecular pathological diagnosis has gradually become an important part of breast cancer diagnosis.

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