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Senile dementia related locus detection method

A technology for senile dementia and detection methods, applied in the direction of biochemical equipment and methods, microbe measurement/inspection, etc., can solve problems such as lost opportunity for treatment and neglect by family members of patients

Inactive Publication Date: 2010-06-23
SHANGHAI GENECORE BIOTECHNOLOGIES
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Problems solved by technology

However, due to the atypicality of the early symptoms of the disease, it is easy to be ignored by the family members of the patient, thus losing the best opportunity for treatment

Method used

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  • Senile dementia related locus detection method
  • Senile dementia related locus detection method
  • Senile dementia related locus detection method

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Embodiment Construction

[0019] In order to better understand the content of the present invention, the following will be further described in conjunction with specific examples.

[0020] 1. Genomic DNA extraction:

[0021] 1.1 Reagents and instruments:

[0022] 1.1.1 Reagents and consumables: Genomic DNA extraction kit (TIANamp BLOOD DNA kit, DP318-03; TIANamp Micro micro sample genomic DNA extraction kit, DP316; TIANamp buccal swab genomic DNA extraction kit, DP322-03) contains : Cell Lysis Solution CL; Buffer GA; Buffer GS; Buffer GB; Buffer GD; Washing Solution PW; Elution Buffer TB; Carrier RNA; RNase-free ddH2O; Proteinase K (20mg / ml); ; Collection tube (2ML); 1.5ML sterile collection tube; Absolute ethanol.

[0023] 1.1.2 Instruments: DENVILLE260 centrifuge, XW-80A vortex mixer, H.H.S pointer electric heating constant temperature water bath.

[0024] 1.2 Extraction steps

[0025] Genomic DNA Extraction from Whole Blood

[0026] 1.2.1 Take 200 μl of anticoagulated whole blood into a 1.5ml E...

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Abstract

The invention relates to a senile dementia related locus detection method, including the steps: sample gene group DNA from human is extracted; a pair of Taqman probes and a pair of primer pairs are respectively designed according to each one of at least two senile dementia related genes, the 5' end and 3' end of the Taqman probe pair are respectively marked with fluorescent reporting group and fluorescent quenching group, and fluorescent quantitative PCR amplification is respectively carried out on the gene group DNA; whether the senile dementia related gene mutates is judged according to the result of the fluorescent quantitative PCR amplification, preferably, the senile dementia related genes are five genes of APOE1 gene, APOE2 gene, CYP46 gene, CTSD gene and IL1A gene. The invention has smart design, simple operation and accurate and reliable detection result and provides reference frame for determining whether further specific health management and subsequent detection means are adopted or not.

Description

technical field [0001] The present invention relates to the technical field of detection of disease-related sites, more specifically, to the technical field of detection methods for senile dementia-related sites, in particular to a detection method for senile dementia-related sites. Background technique [0002] Alzheimer's disease (Alzheimer, AD) is a chronic brain degenerative disease. The clinical manifestations are progressive far and near memory impairment, decline in analysis and judgment, emotional changes, behavioral disturbances, and even confusion, and finally died of pneumonia or urinary tract infection. According to statistics, there are 17-25 million Alzheimer's patients in the world. It is estimated that there are about 6.45 million patients in our country. Moreover, with every 5 years of age increase, the prevalence of AD will increase twice, and 20% of the patients aged 80-85 suffer from senile dementia. It is the fourth cause of death after heart disease,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 陈奕雄赵翊均
Owner SHANGHAI GENECORE BIOTECHNOLOGIES
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